an optical instrument, consisting of lenses or mirrors, by means of which small objects appear larger than they do to the naked eye. Single microscopes consist of a single lens or mirror; or if more lenses or mirrors be made use of, they only serve to throw light upon the object, but do not contribute to enlarge the image of it. Double or compound microscopes are those in which the image of an object is composed by means of more lenses or mirrors than one.
For the principles on which the construction of microscopes depends, see Optics. In the present article, it is intended to describe the finished instrument, with all its varied apparatus, according to the latest improvements; and to illustrate by proper details its uses and importance.
I. Of SINGLE Microscopes.
The famous microscopes made use of by Mr Leeuwenhoek, were all, as Mr Baker affirms us, of the single kind, and the construction of them was the most simple possible; each consisting only of a single lens set between two plates of silver, perforated with a small hole, with a moveable pin before it to place the object on and adjust it to the eye of the beholder. He informs us also, that lenses only, and not globules, were used in every one of these microscopes.
1. The single microscope now most generally known and used is that called Wilson's Pocket Microscope. The body is made of brass, ivory, or silver, and is represented by A.A., B.B. CC is a long fine threaded male screw that turns into the body of the microscope; D a convex glass at the end of the screw. Two concave round pieces of thin brass, with holes of different diameters in the middle of them, are placed to cover the above-mentioned glass, and thereby diminish the aperture when the greatest magnifiers are employed. EE, three thin plates of brass within the body of the microscope; one of which is bent semicircularly in the middle, so as to form an arched cavity for the reception of a tube of glass, the use of the other two being to receive and hold the sliders between them. F, a piece of wood or ivory, arched in the manner of the semicircular plate, and cemented to it. G, the other end of the body of the microscope, where a hollow female screw is adapted to receive the different magnifiers. H is a spiral spring of steel, between the end G and the plates of brass, intended to keep the plates in a right position and counteract the long screw CC. I is a small turned handle, for the better holding of the instrument, to screw on or off at pleasure.
To this microscope belong six or seven magnifying glasses: six of them are set in silver, brass, or ivory, as in the figure K; and marked 1, 2, 3, 4, 5, 6, the lowest numbers being the greatest magnifiers. L is the seventh magnifier, set in the manner of a little barrel, to be held in the hand for the viewing of any larger object. M is a flat slip of ivory, called a slider, with four round holes through it, wherein to place objects between two pieces of glass or Malacca talc, as they appear at dddd. Six such sliders, and one of brass, are usually sold with this microscope, some with objects placed in them, and others empty for viewing any thing that may offer: but whoever pleases to make a collection, may have as many as he desires. The brass slider is to confine any small object, that it may be viewed without crushing or destroying it. N is a tube of glass contrived to confine living objects, such as frogs, fishes, &c. in order to discover the circulation of the blood. All these are contained in a little neat box of fish-skin or mahogany, very convenient for carrying in the pocket.
When an object is to be viewed, thrust the ivory slider, in which the said object is placed, between the two flat brass plates EE: observing always to put that side of the slider where the brass rings are farthest from the eye. Then screw on the magnifying glass you intend to use, at the end of the instrument G; and looking through it against the light, turn the long screw CC, till your object be brought to suit your eye; which will be known by its appearing perfectly distinct and clear. It is most proper to look at it first through a magnifier that can show the whole at once, and afterwards to inspect the several parts more particularly with one of the greatest magnifiers; for thus you will gain a true idea of the whole, and of all its parts. And though the greatest magnifiers can show but a minute portion of any object at once, such as the claw of a flea, the horn of a louse, or the like; yet by gently moving the slider which contains the object, the eye may gradually examine it all over.
As objects must be brought very near the glasses when the greatest magnifiers are made use of, be careful not to scratch them by rubbing the slider against them. Microscope them as you move it in or out. A few turns of the screw CC will easily prevent this mischief, by giving them room enough. You may change the objects in your sliders for any others you think proper, by taking out the brass rings with the point of a penknife; the tales will then fall out, if you but turn the sliders; and after putting what you please between them, by replacing the brass rings you will fasten them as they were before. It is proper to have some sliders furnished with tales, but without any object between them, to be always in readiness for the examination of fluids, salts, sands, powders, the farina of flowers, or any other casual objects of such sort as need only be applied to the outside of the tale.
The circulation of the blood may be easiest seen in the tails or fins of fishes, in the fine membranes between a frog's toes, or both of all in the tail of a water-newt. If your object be a small fish, place it within the tube N, and spread its tail or fin along the side thereof: if a frog, choose such a one as can but just be got into your tube; and, with a pen, or small stick, expand the transparent membrane between the toes of the frog's hind foot as much as you can. When your object is so adjusted that no part of it can intercept the light from the place you intend to view, unscrew the long screw CC, and thrust your tube into the arched cavity, quite through the body of the microscope; then screw it to the true focal distance, and you will see the blood passing along its vessels with a rapid motion, and in a most surprising manner.
The third or fourth magnifiers may be used for frogs or fishes: but for the tails of water-newts, the fifth or sixth will do; because the globules of their blood are twice as large as those of frogs or fish. The first or second magnifier cannot well be employed for this purpose; because the thickness of the tube in which the object lies, will scarce admit its being brought so near as the focal distance of the magnifier.
An apparatus for the purpose of viewing opaque objects generally accompanies this microscope; and which consists of the following parts. A brass arm QR, which is screwed at Q, upon the body of the microscope at G. Into the round hole R, any of the magnifiers suitable to the object to be viewed are to be screwed; and under it, in the same ring, the concave polished silver speculum S. Through a small aperture in the body of the microscope under the brass plates EE, is to slide the long wire with the forceps T: This wire is pointed at one of its ends; and so, that either the points or forceps may be used for the objects as may be necessary. It is easy to conceive, therefore, that the arm at R, which turns by a twofold joint at a and b, may be brought with its magnifier over the object, the light reflected upon it by the application of the speculum, and the true focus obtained by turning of the male screw CC as before directed.—As objects are sometimes not well fixed for view, either by the forceps or point, the small piece shown at V is added, and in such cases answers better: it screws over the point of T; it contains a small round piece of ivory, blackened on one side, and left white upon the other as a contrast to coloured objects, and by a small piece of watch-spring fastens down the objects upon the ivory.
2. Single Microscope by reflection. In fig. 2. A is a microscope, formed of brass fixed upright upon a round wooden base B, or mahogany drawer or case, so as to stand perfectly firm and steady. C is a brass screw, that passes through a hole in the upper limb of the scroll into the side of the microscope D, and screws it fast to the said scroll. E is a concave speculum set in a box of brass, which hangs in the arch G by two small forearms FF, that screw into the opposite sides thereof. At the bottom of this arch is a pin of the same metal, exactly fitted to a hole h in the wooden pedestal, made for the reception of the pin. As the arch turns on this pin, and the speculum turns on the end of the arch, it may, by this twofold motion, be easily adjusted in such a manner as to reflect the light of the sun, of the sky, or of a candle, directly upwards through the microscope that is fixed perpendicularly over it; and by so doing may be made to answer many purposes of the large double reflecting microscope. The body of the microscope may also be fixed horizontally, and objects viewed in that position by any light you choose; which is an advantage the common double reflecting microscope has not. It may also be rendered further useful by means of a slip of glass; one end of which being thrust through between the plates where the sliders go, and the other extending to some distance, such objects may be placed thereon as cannot be applied in the sliders: and then, having a limb of brass that may fasten to the body of the microscope, and extend over the projecting glass a hollow ring wherein to screw the magnifiers, all sorts of subjects may be examined with great convenience, if a hole be made in the pedestal, to place the speculum exactly underneath, and thereby throw up the rays of light. The pocket-microscope, thus mounted, says Mr Baker, "is as easy and pleasant in its use; as fit for the most curious examination of the animalcules and salts in fluids, of the farinae in vegetables, and of the circulation in small animals; in short, is as likely to make considerable discoveries in objects, that have some degree of transparency, as any microscope I have ever seen or heard of."
The brass scroll A is now generally made to unscrew into three parts, and pack with the microscope and apparatus into the drawer of a mahogany pocket-case, upon the lid of which the scroll is made to fix when in use.
The opacous apparatus also, as above described, is applicable this way by reflection. It only consists in turning the arm R (fig. 1.), with the magnifier over the concave speculum below (fig. 2.), or to receive the light as reflected obliquely from it: the silver speculum screwed into R will then reflect the light, which it receives from the glass speculum, strongly upon the object that is applied upon the wire T underneath.
This microscope, however, is not upon the most convenient construction, in comparison with others now made: it has been esteemed for many years past from its popular name, and recommendation by its makers. Its portability is certainly a great advantage in its favour; but in most respects it is superseded by the microscopes hereafter described.
3. Microscope for Opaque Objects, called the Single Fig. 3. Opaque Microscope. This microscope remedies the inconvenience of having the dark side of an object next Microscope. the eye, which formerly was an insurmountable objection to the making observations on opaque objects with any considerable degree of exactness or satisfaction; for, in all other contrivances commonly known, the nearness of the instrument to the object (when glases that magnify much are used) unavoidably overshadows it so much, that its appearance is rendered obscure and indistinct. And notwithstanding ways have been tried to point light upon an object, from the sun or a candle, by a convex glass placed on the side thereof, the rays from either can be thrown upon it in such an acute angle only, that they serve to give a confused glare, but are insufficient to afford a clear and perfect view of the object. But this microscope, by means of a concave speculum of silver highly polished, in whose centre a magnifying lens is placed, such a strong and direct light is reflected upon the object, that it may be examined with all imaginable ease and pleasure. The several parts of this instrument, made either of brass or silver, are as follow.
Through the first side A, passes a fine screw B, the other end of which is fastened to the moveable side C. D is a nut applied to this screw, by the turning of which the two sides A and C are gradually brought together. E is a spring of steel that separates the two sides when the nut is unfastened. F is a piece of brass, turning round in a socket, whence proceeds a small spring tube moving upon a rivet; through which tube there runs a steel wire, one end whereof terminates in a sharp point G, and the other with a pair of pliers H fastened to it. The point and pliers are to thrust into, or take up and hold, any insect or object; and either of them may be turned upwards, as best suits the purpose. I is a ring of brass, with a female screw within it, mounted on an upright piece of the same metal; which turns round on a rivet, that it may be set at a due distance when the least magnifiers are employed. This ring receives the screws of all the magnifiers. K is a concave speculum of silver, polished as bright as possible; in the centre of which is placed a double convex lens, with a proper aperture to look through it. On the back of this speculum a male screw L is made to fit the brass ring I; to screw into it at pleasure. There are four of these concave specula of different depths, adapted to four glasses of different magnifying powers, to be used as the objects to be examined may require. The greatest magnifiers have the least apertures. M is a round object-plate, one side of which is white and the other black: The intention of this is to render objects the more visible, by placing them, if black, on the white side, or, if white, on the black side. A steel spring N turns down on each side to make any object fast; and if rising from the object plate is a hollow pipe to screw it on the needle's point G. O is a small box of brass, with a glass on each side, contrived to confine any living object, in order to examine it: this also has a pipe to screw upon the end of the needle G. P is a turned handle of wood, to screw into the instrument when it is made use of. Q, a pair of brass pliers to take up any object, or manage it with convenience. R is a soft hair-brush for cleaning the glasses, &c. S is a small ivory box for tacks, to be placed, when wanted, in the small brass box O.
When you would view any object with this microscope, screw the speculum, with the magnifier you think proper to use, into the brass ring I. Place your object, either on the needle G in the pliers H, on the object-plate M, or in the hollow brass box O, as may be most convenient: then holding up your instrument by the handle P, look against the light through the magnifying lens; and by means of the nut D, together with the motion of the needle, by managing its lower end, the object may be turned about, raised, or depressed, brought nearer the glass, or removed farther from it, till you find the true focal distance, and the light be seen strongly reflected from the speculum upon the object, by which means it will be shown in a manner surprisingly distinct and clear; and for this purpose the light of the sky or of a candle will answer very well. Transparent objects may also be viewed by this microscope; only observing, that when such come under examination, it will not always be proper to throw on them the light reflected from the speculum; for the light transmitted through them, meeting the reflected light, may together produce too great a glare. A little practice, however, will show how to regulate both lights in a proper manner.
4. Ellis's Single and Aquatic Microscope. Fig. 4. re-Fig. 4 presents a very convenient and useful microscope, contrived by Mr John Ellis, author of An Essay upon Corallines, &c. To practical botanists, observers of animalcula, &c., it possesses many advantages above those just described. It is portable, simple in its construction, expeditious, and commodious in use. K represents the box containing the whole apparatus: it is generally made of fish-skin; and on the top there is a female screw, for receiving the screw that is at the bottom of the pillar A: this is a pillar of brass, and is screwed on the top of the box. D is a brass pin which fits into the pillar; on the top of this pin is a hollow socket to receive the arm which carries the magnifiers; the pin is to be moved up and down, in order to adjust the lenses to their focal or proper distance from the object. [N.B. In the representations of this microscope, the pin D is delineated as passing through a socket at one side of the pillar A; whereas it is usual at present to make it pass down a hole bored through the middle of the pillar.] E, the bar which carries the magnifying lens; it fits into the socket X, which is at the top of the pin or pillar D. This arm may be moved backwards and forwards in the socket X, and sideways by the pin D; so that the magnifier, which is screwed into the ring at the end E of this bar, may be easily made to traverse over any part of the object that lies on the stage or plate B. FF is a polished silver speculum, with a magnifying lens placed at the centre thereof, which is perforated for this purpose. The silver speculum screws into the arm E, as at F. G, another speculum, with its lens, which is of a different magnifying power from the former. H, the semicircle which supports the mirror I; the pin R, affixed to the semicircle H, passes through the hole which is towards the bottom of the pillar A. B, the stage, or the plane, on which the objects are to be placed; it fits into the small dove-tailed arm which is at the upper end of the pillar DA. C, a plane glass, with a small piece of black silk stuck on it; this glass is to lay in a groove made in the stage C. M, a hollow glass to be laid occasionally on the stage instead. stead of the plane glass C. L., a pair of nippers.
These are fixed to the stage by the pin at bottom; the steel wire of these nippers slides backwards and forwards in the socket, and this socket is moveable upwards and downwards by means of the joint, so that the position of the object may be varied at pleasure. The object may be fixed in the nippers, stuck on the point, or affixed, by a little gum-water, &c. to the ivory cylinder N, which occasionally screws to the point of the nippers.
To use this microscope: Take all the parts of the apparatus out of the box; then begin by screwing the pillar A to the cover thereof; pass the pin R of the semicircle which carries the mirror through the hole that is near the bottom of the pillar A; push the stage into the dovetail at B, slide the pin into the pillar (see the N. B. above); then pass the bar E through the socket which is at the top of the pin D, and screw one of the magnifying lenses into the ring at F. The microscope is now ready for use: and though the enumeration of the articles may lead the reader to imagine the instrument to be of a complex nature, we can safely affirm that he will find it otherwise. The instrument has this peculiar advantage, that it is difficult to put any of the pieces in a place which is appropriated to another. Let the object be now placed either on the stage or in the nippers L, and in such manner that it may be as nearly as possible over the centre of the stage: bring the speculum F over the part you mean to observe; then throw as much light on the speculum as you can, by means of the mirror I, and the double motion of which it is capable; the light received on the speculum is reflected by it on the object. The distance of the lens F from the object is regulated by moving the pin D up and down, until a distinct view of it is obtained. The best rule is, to place the lens beyond its focal distance from the object, and then gradually to slide it down till the object appears sharp and well defined. The adjustment of the lenses to their focus, and the distribution of the light on the object, are what require the most attention: on the first the distinctness of the vision depends; the pleasure arising from a clear view of the parts under observation is due to the modification of the light. No precise rule can be given for attaining accurately these points; it is from practice alone that ready habits of obtaining these necessary properties can be acquired, and with the assistance of this no difficulty will be found.
5. A very simple and convenient microscope for botanical and other purposes, though inferior in many respects to that of Mr Ellis, was contrived by the ingenious Mr Benjamin Martin, and is represented at fig. 5. where AB represents a small arm supporting two or more magnifiers, one fixed to the upper part as at B, the other to the lower part of the arm at C; these may be used separately or combined together. The arm AB is supported by the square pillar IK, the lower end of which fits into the socket E of the foot FG; the stage DL is made to slide up and down the square pillar; H, a concave mirror for reflecting light on the object.—To use this microscope, place the object on the stage, reflect the light on it from the concave mirror, and regulate it to the focus, by moving the stage nearer to or farther from the lens at B. The ivory sliders pass through the stage; other objects may be fixed in the nippers MN, and then brought under Microscope, the eye-glasses; or they may be laid on one of the glasses which fit the stage. The apparatus to this instrument consists of three ivory slides; a pair of nippers; a pair of forceps; a flat glass and a concave ditto, both fitted to the stage.
The two last microscopes are frequently fitted up with a toothed rack and pinion, for the more ready adjustment of the glasses to their proper focus.
6. Withering's portable Botanic Microscope. Fig. 6. Fig. 6 represents a small botanical microscope contrived by Dr Withering, and described by him in his Botanical Arrangements. It consists of three brass plates, ABC, which are parallel to each other; the wires D and E are riveted into the upper and lower plates, which are by this means united to each other; the middle plate or stage is moveable on the aforeaid wires by two little sockets which are fixed to it. The two upper plates each contain a magnifying lens, but of different powers; one of these confines and keeps in their places the fine point F, the forceps G, and the small knife H.—To use this instrument, unscrew the upper lens, and take out the point, the knife, and the forceps; then screw the lens on again, place the object on the stage, and then move it up or down till you have gained a distinct view of the object, as one lens is made of a shorter focus than the other; and spare lenses of a still deeper focus may be had if required. This little microscope is the most portable of any. Its principal merit is its simplicity.
7. Botanical Lenses or Magnifiers. The haste with which botanists, &c. have frequently occasion to view objects, renders an extempore pocket-glass indispensible necessary. The most convenient of any yet constructed, appears to be that contrived, in regard to the form of the mounting, by Mr Benjamin Martin; and is what he called a Hand Microscope, because it is well adapted for viewing all the larger sort of small objects universally, and by only three lenses it has seven different magnifying powers.
Fig. 7. represents the case with the three frames and Fig. 7. lenses, which are usually of 1, 1½, and two inches focus: they all turn over each other, and shut into the case, and are turned out at pleasure.
The three lenses singly, afford three magnifying powers; and by combining two and two, we make three more: for d with e makes one, d with f another, and e with f a third; which, with the three singly, make five; and lastly, all three combined together make another; so that upon the whole, there are seven powers of magnifying with these glasses only.
When the three leaves are combined, it is better to turn them in, and look through them by the small apertures in the sides of the case. The eye in this case is excluded from extra light; the aberration of the superfluous rays through the glasses is cut off; and the eye coincides more exactly with the common axes of the lenses.
A very useful and easy kind of microscope (described by Joblot, and which has been long in use), adapted chiefly for viewing, and confining at the same time, any living insects, small animals, &c. is shown at fig. 8. where A represents a glass tube, about 1½ inch diameter, and 2 inches high. B, a case of brass or wood, containing a sliding tube, with two or three magnifying Microscope ing glasses that may be used either separately or combined. In the inside, at the bottom, is a piece of ivory, black and white on opposite sides, that is occasionally removed, and admits a point to be screwed into the centre. The cap uncrews at D, to admit the placing of the object; the proper distance of the glasses from the object is regulated by pulling up or down the brass tube E at top containing the eye-glasses.
This microscope is particularly useful for exhibiting the well-known curious curculio imperialis, vulgarly called the diamond beetle, to the greatest advantage; for which, as well as for other objects, a glass bottom, and a polished reflector at the top, are often applied, to condense the light upon the object. In this case, the stand and brass-bottom F, as shown in the figure, are taken away by unfreewing.
9. Mr Lyonet's Single Anatomical Dissecting Microscope.—Fig. 9 represents a curious and extremely useful microscope, invented by that gentleman for the purpose of minute dissections, and microscopic preparations. This instrument must be truly useful to amateurs of the minute of insects, &c., being the best adapted of any for the purposes of dissection. With this instrument Mr Lyonet made his very curious microscopical dissection of the chenille de faule, as related in his Traité Anatomique de la chenille qui rouge le bois de faule, 4to.
AB is the anatomical table, which is supported by a pillar NO; this is screwed on the foot CD. The table AB is prevented from turning round by means of two steady pins. In this table or board there is a hole G, which is exactly over the centre of the mirror EF, that is to reflect the light on the object; the hole G is designed to receive a flat or concave glass, on which the objects for examination are to be placed.
RXZ is an arm formed of several balls and sockets, by which means it may be moved in every possible situation; it is fixed to the board by means of the screw H. The last arm IZ has a female screw, into which a magnifier may be screwed as at Z. By means of the screw H, a small motion may be occasionally given to the arm IZ, for adjusting the lens with accuracy to its focal distance from the object.
Another chain of balls is sometimes used, carrying a lens to throw light upon the object; the mirror is likewise so mounted, as to be taken from its place at K, and fitted on a clamp, by which it may be fixed to any part of the table AB.
To use the Dissecting Table.—Let the operator fit with his left side near a light window; the instrument being placed on a firm table, the side DH towards the stomach, the observations should be made with the left eye. In dissecting, the two elbows are to be supported by the table on which the instrument rests, the hands resting against the board AB; and in order to give it greater stability (as a small shake, though imperceptible to the naked eye, is very visible in the microscope), the dissecting instruments are to be held one in each hand, between the thumb and two fore-fingers.
II. Of Double Microscopes, commonly called Compound Microscopes.
Double microscopes are so called, from being a combination of two or more lenses.
The particular and chief advantages which the compound microscopes have over the single, are, that the objects are represented under a larger field of view, and with a greater amplification of reflected light.
1. Culpeper's Microcope.—The compound microscope, originally contrived by Mr Culpeper, is represented at fig. 10. It consists of a large external brass body A, Fig. 10: B, C, D, supported upon three scrolls, which are fixed to the stage EF; the stage is supported by three larger scrolls, that are screwed to the mahogany pedestal GH. There is a drawer in the pedestal, which holds the apparatus. The concave mirror I is fitted to a socket in the centre of the pedestal. The lower part LMCD of the body forms an exterior tube, into which the upper part of the body ABLM slides, and may be moved up or down, so as to bring the magnifiers, which are screwed on at N, nearer to or farther from the object.
To use this microscope: Screw one of the buttons, which contains a magnifying lens, to the end N of the body; place the slider, with the objects, between the plates of the slider-holder. Then, to attain distinct vision, and a pleasing view of the object, adjust the body to the focus of the lens you are using, by moving the upper part gently up and down, and regulate the light by the concave mirror.
For opaque objects, two additional pieces must be used. The first is a cylindrical tube of brass (represented at L, fig. 11.), which fits on the cylindrical part at N of the body. The second piece is the concave speculum h; this is to be screwed to the lower end of the aforementioned tube: the upper edge of this tube should be made to coincide with the line which has the same number affixed to it as to the magnifier you are using; e.g., if you are making use of the magnifier marked 5, slide the tube to the circular line on the tube N that is marked allo with No 5. The slider-holder should be removed when you are going to view opaque objects, and a plane glass should be placed on the stage in its stead to receive the object; or it may be placed in the nippers, the pin of which fits into the hole in the stage.
The apparatus belonging to this microscope consists of the following particulars: viz. Five magnifiers, each fitted in a brass button; one of these is seen at N, fig. 10. Six ivory sliders, five of them with objects. A brass tube, to hold the concave speculum. The concave speculum in a brass box. A fish pan. A set of glass tubes. A flat glass fitted to the stage. A concave glass fitted to the stage. A pair of forceps. A steel wire, with a pair of nippers at one end and a point at the other. A small ivory cylinder, to fit on the pointed end of the aforementioned nippers. A convex lens, moveable in a brass semicircle; this is affixed to a long brass pin, which fits into a hole on the stage.
The construction of the foregoing microscope is very simple, and it is easy to use; but the advantages of the stage and mirror are too much confined for an extensive application and management of all kinds of objects. Its greatest recommendation is its cheapness; and to those who are desirous of having a compound microscope at a low price, it may be acceptable.
2. Cuff's Microscope.—The improved microscope next in order is that of Mr Cuff. Besides remedying the disadvantages above mentioned, it contains the addition... Microscope addition of an adjusting screw, which is a considerable improvement, and highly necessary to the examination of objects under the best defined appearance from the glasses. It is represented at fig. 11, with the apparatus that usually accompanies it. A, B, C, shows the body of this microscope; which contains an eye-glass at A, a broad lens at B, and a magnifier which is screwed on at C. The body is supported by the arm DE, from which it may be removed at pleasure. The arm DE is fixed on the sliding bar F, and may be raised or depressed to any height within its limits. The main pillar ab is fixed in the box b e; and by means of the brass foot d is screwed to the mahogany pedestal XY, in which is a drawer containing all the apparatus. O is a milled-headed screw, to tighten the bar F when the adjusting screw c g is used. p q is the stage, or plate, which carries the objects; it has a hole at the centre n. G, a concave mirror, that may be turned in any direction, to reflect the light of a candle, or the sky, upon the object.
To use this microscope: Screw the magnifier you intend to use to the end C of the body; place the slider-holder P in the hole n, and the slider with the object between the plates of the slider-holder; set the upper edge of the bar DE to coincide with the divisions which correspond to the magnifier you have in use, and pinch it by the milled nut; now reflect a proper quantity of light upon the object, by means of the concave mirror G, and regulate the body exactly to the eye and the focus of the glasses by the adjusting screw c g.
To view opaque objects, take away the slider-holder P, and place the object on a flat glass under the centre of the body, or on one end of the jointed nippers o p. Then screw the silver concave speculum h to the end of the cylinder L, and slide this cylinder on the lower part of the body, so that the upper edge thereof may coincide with the line which has the same mark with the magnifier that is then used: reflect the light from the concave mirror G to the silver speculum, from which it will again be reflected on the object. The glasses are to be adjusted to their focal distance as before directed.
The apparatus consists of a convex lens H, to collect the rays of light from the sun or a candle, and condense them on the object. L a cylindrical tube, open at each side, with a concave speculum screwed to the lower end h. P the slider-holder: this consists of a cylindrical tube, in which an inner tube is forced upwards by a spiral spring; it is used to receive an ivory slider K, which is to be slid between the plates h and i. The cylinder P fits the hole n in the stage; and the hollow part at k is designed to receive a glass tube. R is a brass cone, to be put under the bottom of the cylinder P, to intercept occasionally some of the rays of light. S, a box containing a concave and a flat glass, between which a small living insect may be confined: it is to be placed over the hole n. T a flat glass, to lay any occasional object upon; there is also a concave one for fluids. O is a long steel wire, with a small pair of pliers at one end, and a point at the other, designed to stick or hold objects: it flips backwards and forwards in the short tube o; the pin p fits into the hole of the stage. W, a little round ivory box, to hold a supply of talc and rings for the microscope sliders. V, a small ivory cylinder, that fits on the pointed end of the steel wire: it is designed for opaque objects. Light-colored ones are to be stuck upon the dark side, and vice versa. M, a fish-pan, wherein to fasten a small fish, to view the circulation of the blood: the tail is to be spread across the oblong hole k at the small end, and tied fast, by means of a ribbon fixed thereto; the knob l is to be thrown through the slit made in the stage, that the tail may be brought under the magnifier.
3. This microscope has received several material improvements from Mr Martin, Mr Adams, &c. By an alteration, or rather an enlargement, of the body of the tube which contains the eye-glasses, and also of the eye-glasses themselves, the field of view is made much larger, the mirror below for reflecting light is made to move upon the same bar with the stage; by which means the distance of it from the stage may be very easily and suitably varied. A condensing glass is applied under the stage in the slider-holder, in order to modify and increase the light that is reflected by the mirrors below from the light of a candle or lamp. It is furnished also with two mirrors in one frame, one concave and the other plane, of glass silvered; and by simply unscrewing the body, the instrument, when desired, may be converted into a single microscope. Fig. 12 is a representation of the instrument thus improved; and the following is the description of it, as given by Mr Adams in his Essays.
AB represents the body of the microscope, containing a double eye-glass and a body-glass: it is here shown as screwed to the arm CD, from whence it may be occasionally removed, either for the convenience of packing, or when the instrument is to be used as a single microscope.
The eye-glasses and the body-glasses are contained in a tube which fits into the exterior tube AB; by pulling out a little this tube when the microscope is in use, the magnifying power of each lens is increased.
The body AB of the microscope is supported by the arm CD; this arm is fixed to the main pillar CF, which is screwed firmly to the mahogany pedestal GH; there is a drawer to this pedestal, which holds the apparatus.
NIS, the plate or stage which carries the slider-holder KL; this stage is moved up or down the pillar CF, by turning the milled nut M; this nut is fixed to a pinion, that works in a toothed rack cut on one side of the pillar. By means of this pinion, the stage may be gradually raised or depressed; and the object adjusted to the focus of the different lenses.
KL is a slider-holder, which fits into a hole that is in the middle of the stage NIS; it is used to confine and guide either the motion of the sliders which contain the objects, or the glasses tubes that are designed to confine small fishes for viewing the circulation of the blood. The sliders are to be passed between the two upper plates, the tubes through the bent plates.
L is a brass tube, to the upper part of which is fixed the condensing lens before spoken of; it fits into the under part of the slider-holder KL, and may be set at different distances from the object, according to its distance from the mirror or the candle.
O is the frame which holds the two reflecting mir- Microscopes, one of which is plane, the other concave. These mirrors may be moved in various directions, in order to reflect the light properly, by means of the pivots on which they move, in the semicircle QSR, and the motion of the semicircle itself on the pin S; the concave mirror generally answers best in the day-time; the plane mirror combines better with the condensing lens, and a lamp or candle. At D there is a socket for receiving the pin of the arm Q (fig. 31.), to which the concave speculum, for reflecting light on opaque objects, is fixed. At S is a hole and slit for receiving either the nippers L (fig. 31.) or the fish-pan I; when these are used, the slider-holder must be removed. T, a hole to receive the pin of the convex lens M.
To use this microscope: Take it out of the box. Screw the body into the round end of the upper part of the arm CD. Place the brass sliders, which contain the magnifiers, into the dove-tailed slit which is on the under side of the aforeaid arm, as seen at E, and slide it forwards until the magnifier you mean to use is under the centre of the body: opposite to each magnifier in this slit there is a notch, and in the dove-tailed part of the arm CD there is a spring, which falls into the above-mentioned notch, and thus makes each magnifier coincide with the centre of the body. Pass the ivory slider you intend to use between the upper plates of the slider-holder KL, and then reflect as strong a light as you can on the subject by means of one of the mirrors; after this, adjust the object to the focus of the magnifier and your eye, by turning the milled screw M, the motion of which raises and depresses the stage NIS. The degree of light necessary for each object, and the accuracy required in the adjustment of the lenses to their proper focal distance from the object, will be easily attained by a little practice.
When opaque objects are to be examined, remove the slider-holder, and place the object on a flat glass, or fix it to the nippers L, the pin of these fit into the hole on the stage; screw the concave speculum R into the arm Q (fig. 31.), and then pass the pin of this arm through the socket D (fig. 12.); the light is now to be reflected from the concave mirror to the silver speculum, and from this down on the object. No exact rule can be given for reflecting the light on the object; we must therefore refer the reader to the mother of all arts, practice. The speculum must be moved lower or higher, to suit the focus of the different magnifiers and the nature of the object.
The foregoing directions apply equally to the using of this instrument as a single microscope; with this difference only, that the body AB is then removed, and the eye is applied to the upper surface of the arm CD, exactly over the magnifiers.
This microscope is sometimes made with the following alterations, which are supposed to make it still more convenient and useful. The arm CD that carries the body and magnifiers is made both to turn on a pin, and to slide backwards and forwards in a socket at C; so that, instead of moving the objects below on the stage, and disturbing them, the magnifiers are more conveniently brought over any part of the objects as desired. The condensing glass is made larger, and slides upon the square bar CF quite distinct from the stage, like the mirrors below; and it is thereby made useful for any other objects that may be applied on Microscopes glasses fitted to the stage, as well as those put into the slider-holder K. It is thereby not confined to this stage alone as in the preceding. When the body AB is taken away, the arm CD may be slid away from its bar, with the magnifiers, and the forceps, wire, and joint, applied to it; and it thereby serves the purpose of a small single or opaque hand microscope, for any object occasionally applied to this wire. The magnifiers in the slider E are mounted in a wheel case, which perhaps prevents its being in the way so much as the long slider E before described.—This contrivance is represented at X, fig. 12.
4. Martin's New Universal Compound Microscope.—This instrument was originally constructed by Mr B. Martin, and intended to comprise all the uses and advantages of the single, compound, opaque, and aquatic microscopes. The following is a description of it.
Fig. 13. is a representation of the instrument placed up for use. ABCD is the body of the microscope: which consists of four parts, viz. AB the eyepiece, or that containing the eye-glasses, and is screwed into C, which is a moveable or sliding tube on the top; this inner tube contains the body-glass screwed into its lower part. D is the external tube or case, in which the other slides up and down in an easy and steady manner. This motion of the tube C is useful to increase and decrease the magnifying power of the body-glass when thought necessary, as before mentioned. E is a pipe or snout screwed on to the body of the microscope D, and at its lower part, over the several magnifying lenses hereafter described. FGHI is the square stem of the microscope, upon which the stage R moves in an horizontal position, upwards or downward, by means of the fine rack work of teeth and pinion. KL is a strong solid joint and pillar, by which the position of the instrument is readily altered from a vertical one to an oblique or to a perfectly horizontal one as may be required: it is thus well adapted to the case of the observer either sitting or standing; and as it is very often convenient to view objects by direct unreflected light, when the square stem FI is placed in a horizontal position for this purpose, the mirror T is then to be taken off in order to prevent the obstruction of the rays. M is a circular piece of brass, serving as a base to the pillar. NOP, the tripod or foot by which the whole body of the microscope is steadily supported; it folds up when packed into the case. W is a brass frame, that contains the condensing lens, and acts in conjunction with the large concave and plane mirrors below at T; the reflected rays from which, either of the common light or of that of a candle or lamp, it agreeably modifies, and makes steady in the field of view.
The particulars of the apparatus to this microscope are as follow: Q is a circular brass box, containing six magnifiers or object lenses, numbered 1, 2, 3, 4, 5, 6; the digits of which appear severally through a small round hole in the upper plate of it. To the upper side is fixed a small circle of brass, by which it is connected with, and screwed into, the round end of the arm abc; which is a long piece of brass, and moves through either by teeth or pinion, or not, as may be desired, in ef; which is a socket on the upper part of the pillar, and admits, with a motion both easy and steady, the R is a fixed stage, upon which the objects to be viewed are to be placed; it is firmly fastened to the square pillar, which is moved by the rack-work. In the middle is a large circular hole, for receiving concave glasses, with fluids, &c., it has also a sliding spring frame to fasten down slips of glass or other things: at abc are three small sockets or holes, intended to receive several parts of the apparatus. S is the refractor, or illuminating lens, for converging the sun's rays upon opaque objects laid upon the stage R. To this purpose it moves on a semicircle upon a long shaft g, in a spring socket h, in the arm i; this arm moving every way by a stout pin k in the socket a of the stage. In this manner it is easily adjusted to any position of the sun, candle, &c.—T, the reflecting-glass frame, containing a concave and plane speculum, which is moved upon the square pillar by the hand. The use of it is to illuminate all transparent objects that are applied to the stage above.
Fig. 14. No. 1. is an auxiliary moveable stage; which by means of a pin k is placed in the hole a of the stage R, and can be moved in a horizontal direction over the whole field of the stage. In this stage, there are three circular holes with shouldered bottoms; a large one in the middle, and on each side a small one, for the reception of the three following necessary articles: No. 2, a watch-glass to be placed in the large hole, to hold fluids containing animalcules, &c.; a circular piece of ivory, No. 3, one side of which is black, the other white, to support opaque objects of different contrasted colours; and circular plane and concave glasses, No. 4, for extemporaneous transparent objects.—The same use is made of the other small hole as of the large one, only in a lesser degree, to receive small concave glasses, plates, &c.
No. 5. is the silvered speculum, called a liberkhun, which makes the single opaque microscope, by being screwed to the slider abcd (fig. 13.) in room of the box of lenses Q, and the body AE above it. The chief use of this is to view very small objects strongly illuminated near the compounded focus of the mirror T (fig. 13.). No. 6. is the forceps or pliers, for holding such kind of objects, and by which they can be applied very readily to the focus of the lens in the liberkhun. They have a motion all ways by means of the spring socket a, the joint b, and the shank c: they are placed in the socket c of the fixed stage R (fig. 13.). No. 7. is a small piece of ivory, to be placed upon the pointed end of the pliers: it is black upon one side, and white upon the other, to receive opaque objects.
No. 8. is a liberkhun of a larger size than that first mentioned, with a hole in its centre: this is screwed into No. 9, the hole a of a brass ring, fastened to a long wire b; which moves up and down in the spring socket b of the stage R, in which it also moves sideways; and thus, with the body AE above, forms an aquatic compound microscope for viewing all sorts of objects in water and other fluids placed under it in the watch-glass No. 2. on the stage.
No. 11. is a cone, with a proper aperture a to exclude superfluous light, that would disturb a critical observation of a curious object; it is placed on the under side of the fixed stage R.
No. 12. is what is usually called a bug-box, consisting of a concave glass with a plane one screwed over it; by means of which a bug, louse, flea, &c. may be secured and viewed alive. It is to be placed on either of the stages R (fig. 13.), or No. 1. (fig. 14.).
No. 13. is the fifth pan. In the long concave body ab, a fish may be so confined by the ribbon c, that the transparent tail may be in part over the slit or hole at a. In this state, it is placed on the stage R, with the pin d in the hole c of the stage, and moves freely and horizontally for viewing the circulation of the blood, &c.
No. 14. is the slider-holder that is placed on the stage R: it receives the sliders and tubes when filled with transparent objects, to be viewed either by the compound or single microscope.
No. 15. represents the ivory slider, to hold the objects between the tacks as usual.
No. 16. is a useful auxiliary slider framed in brass. In this slider small concave glasses are cemented; and a slip of plane glass slides over them; by which any small living object, as mites, &c., may be confined without injury, and deliberately viewed.
No. 17. represents a set of glass tubes, three in number, one within another; they are useful for small tadpoles, water newts, eels, &c., when the circulation of the blood is to be viewed. There is a small hole at one end of each tube, that serves to admit the air; for when they are filled with water, the other end is stoppered with a cork.
No. 18. is a small ivory box, containing spare tacks and wires, to supply the sliders with occasionally.
No. 19. a brass cell or button, containing a very small lens, properly set between two small plates of brass, that it may be brought very near to the object when viewed therewith as a single microscope. This magnifier is screwed into the same hole as the wheel of five magnifiers Q are (fig. 13.).
No. 20. is a lens, adapted to view and examine objects, by magnifying them sufficiently, so as to be able to apply them to the microscope for inspection: on this account it is called the explorator.
The preceding are the chief articles of the apparatus: which, on account of their being somewhat different from what is applied to other microscopes, we have been thus particular in describing. In using the microscope, and while viewing objects by either the single or compound instrument, the focal distances of the magnifiers are made perfectly exact by turning of the pinion at the nut w, in one way or the other, very gently in the teeth of the rack-work at X (fig. 13.).
It is necessary that the centres of the object-lenses or magnifiers, the stage, and the mirrors at bottom, should all be in a right line in the axis of the microscope, when opaque objects are to be viewed, that are placed upon the ivory piece No. 7. or the forceps No. 6, and all other such sort of objects which are placed in the centre of the stage R, or slider-holder No. 14.: But when aquatic or living objects, which require a great space to move in, are to be viewed, then the horizontal motion at ef (fig. 13.) is made use of, and the view may be extended laterally over the whole of the diameter of the object or field of view; and by putting the arm abcd forward or backward in its socket ef, the view is extended in the contrary direction. Microscope direction equally well; and in this manner the whole of the objects may be viewed without the least disturbance.
As the bras arm \( abcd \) may be brought to the height of three or four inches above the stage \( R \); so, by means of the rack-work motion of the stage, a lens of a greater focal distance than the greatest in the wheel \( Q \) may be occasionally applied in place of the wheel, and thereby the larger kind of objects be viewed; the instrument becoming, in this case, what is called a megalocope.
In viewing moving living objects, or even fixed ones, when nice motions are requisite, a rack-work and pinion is often applied to the arm \( abcd \); the arm is cut out with teeth; and the pinion, as shown at \( X \), is applied to work it. This acts but in one direction; and, in order to produce an equally necessary motion perpendicular to this, rack-work and pinion is applied tangent-wise to the stage, which is then jointed.
What has been related above respects the construction of those denominated parlour microscopes, in contradiction to those which are portable; their dimensions, however, have been considerably reduced by opticians, in order to render them fit for the pocket; and as they are for the most part constructed on nearly the same principles as those which have been already described, what has been said will sufficiently instruct our readers in using any pocket microscope whatever. Only it may be observed, that in those reduced instruments, both the field of view and the magnifying power are proportionally diminished.
We shall conclude the account of this sort of microscope with descriptions of a very portable pocket apparatus of microscopic instruments, and of a new microscopic pocket telescope, both invented by the late Mr B. Martin, and since made by most instrument-makers in London.
The former is represented at fig. 15. It consists of two parts, viz. the body \( ab \), and the pedestal \( ik \), which is joined by a screw at the part between \( b \) and \( i \). It consists of three cylindric tubes, viz. (1.) the exterior tube, or case, \( ab \); (2.) a middle tube \( cd \); and (3.) the interior tube \( fg \). The middle tube \( cd \) is the adjuster; and is connected with the outer tube by the rack-work of teeth and pinion, as shown at \( e \); by which means it is moved up and down at pleasure through the smallest space, and carries with it the internal tube \( fg \). The interior tube \( fg \) receives on its lower part at \( b \) the several capsules or boxes \( 2, 3, 4, 5 \), (fig. 16.) which contain the object lenses or magnifiers.
The method of using this compound microscope in the perpendicular position, is as follows: The stage \( No \) 1. is put within the exterior tube at \( b \). Under the springs are applied the four ivory sliders, which contain a variety of transparent objects; then move the interior tube \( fg \) up and down with the hand, till you discern the object in the slider, and there let it rest. After this, turn the pinion at \( e \) very tenderly one way or the other, till you obtain a perfect view of the transparent objects properly illuminated, from a mirror contained in the pedestal or stand \( ik \), suspended upon, and moveable about, the points of two screws (\( II \)). \( No \) 6. (fig. 16.) represents a moveable stage, which is placed in the spring socket \( m \). It contains a concave glass, for the reception of animal cules in fluids; and has the advantage of bringing any part into view by moving the handle at \( n \). If living and moving objects are required to be shown, they must be confined in the concave, by putting a glass cover, \( No \) 7. upon the stage; and then a small spider, a louse, a flea, bug, &c. may be seen, and the motion or circulation of the blood, &c. observed with surprising distinctness.
To view the circulation of the blood in the most eminent degree, it must be done by placing small frogs, tadpoles, water-newts, fishes, &c. in a tube as represented \( No \) 8. (fig. 17.) which tube is placed in the holes Fig. 17. \( a \) in the opposite sides of the case \( ab \), fig. 15. in the lower part.—\( No \) 9. (fig. 16.) is a pair of pincers or pliers \( d \), for holding any object; the other end of the steel wire is pointed to receive a piece of ivory \( b \), with one end black, and the other white, on which you stick objects of different hue: this also, when used, is placed in the spring socket \( m \).
To use this instrument as a compound opaque, you screw off the body part \( ab \), and screw to it the handle \( r \) (fig. 16.); by this means you may hold the microscope in a horizontal position, as shown in the figure. The silver dish or speculum (which is contained in the bottom or base \( k \), fig. 15.), is then screwed on at \( b \). \( No \) 9. is placed in the spring socket \( m \), and adjusted backward and forward in \( m \), till the reflected light from the speculum falls in a proper manner on the opaque object. Either of the 4 magnifiers, 2, 3, 4, 5, may be used, and brought to a proper focus, as before described by the tooth and pinion \( e \) (fig. 15.). If you take off the opaque apparatus, and apply the stage \( No \) 1. (fig. 16.) with an ivory slider, and at the end \( b \) terew in either of the two lenses, \( No \) 10. (which are distinguished by the name of illuminators), the microscope being held up to the light (and properly adjusted), the whole field of view will be strongly illuminated, and present a most pleasing appearance of any transparent object. These two convex lenses are of different focuses, and are to be used singly or together; \( No \) 2. being the greatest magnifier, will require the object to be strongly illuminated, and of course both the lenses must be used together. By candle-light, this method of viewing transparent objects will prove very entertaining; by screwing the handle \( r \) into the part \( s \) of \( No \) 10., it becomes a delightful hand megalocope for viewing flowers, fossils, shells, &c.; and each lens, as before mentioned, having a different focus, produces two magnifying powers used singly, and when combined a third.
The manner of using this instrument as a single microscope (like Willson's) is represented in fig. 17., where the button or magnifier at each is to be screwed off, and the circular piece \( No \) 11. is screwed in its place. This piece has a spring socket made to receive the slider-holder \( No \) 12. \( No \) 13. is a circular piece of brass, with a long shank and spring, and is introduced through the outside tube \( ab \) at \( t \). \( No \) 2, 3, 4, 5, are screwed occasionally in the centre of this piece, and used as single lenses with ivory sliders, &c. \( No \) 14. contains a lens of a great magnifying power, for viewing very minute objects: to render this instrument the most complete single opaque microscope, you have only to screw into \( No \) 13. Microscope, the silver speculum No. 15, which has a small lens set in its centre. The slider-holder No. 12 is taken out of No. 11, and the pincers or nippers d b, being detached from the other part of No. 9, are passed through the long spring socket No. 11, and ready to receive any opaque body in the pincers, or on the black and white piece of ivory. To the large screw of No. 13, are applied the two lenses No. 10, which make it the completest microscope that can be desired.
The handle r contains the four ivory sliders with objects.
The fragreen case which contains this universal microscope and its apparatus, is six inches long, three inches wide, two inches deep; and weighs together 16 ounces. "Thus (says Mr Martin) so small, so light, so portable, and yet so universally complete, is this pocket microscopic apparatus, that you find nothing material in the large three-pillared microscope, the opaque microscope, Wilson's single microscope, and the aquatic microscope, all together, which you have not in this; besides some very considerable advantages in regard to the field of view, &c. which they have not (a)."
This inventive artist having contrived a construction of the compound microscope so small as to admit of being packed in a common walking cane, thought next of introducing the same instrument into the inside of what he called his Pocket Three-brafs drawer Achromatic Telescope. The same eye glafes that serve the purpose of a telescope, answer as the compound magnifier, for viewing transparent and opaque objects in a microscope.
Fig. 18, 19, Fig. 18, 19, 20, represent the telescope separated by unscrewing it at m, in order that the whole of the necessary parts in use may be exhibited. Fig. 19 represents the exterior tube, which is of mahogany, and its rims of brafs. It is detached from the rest of the telescope, as not making any part of the microscope. The brafs cover k l, that shuts up the object-glasfs of the telescope, is also the box which contains the two-wheel object frames, and a small plain reflecting mirror.
In fig. 20, A is the cover taken off, by unscrewing the top part: The mirror B is taken out; and also, by unscrewing the bottom part, the two circular wheels, with the objects shown in C and D.
Fig. 18, is a representation of the three internal brafs sliding tubes of the telescope, which form the microscopic part. The tubes are to be drawn out as shown in this figure; then, at the lower end of the large tube in the inside, is to be pulled out a short tube b c, that serves as a kind of stage to hold the wheels with objects, and support the reflecting mirror. This tube is to be partly drawn out, and turned so that the circular hole that is pierced in it may coincide with a similar hole that is cut in the exterior tube. This tube is represented as drawn out in the figure; and the mirror B placed therein, and the wheel with Microscope. transparent objects. C (fig. 20.) represents the wheel with transparent objects, and D the wheel with opaque objects. They are both made of ivory; and turn round upon a centre brafs pin slit upon the top, which fits upon the edge of the tube; which tube is then to be pushed up into the telescope tube, so that its lower end may rest upon the upper edge of the wheel according to its view at a fig. 18.
In viewing the objects, the second brafs tube of the telescope must be pushed down, till its milled edge at top falls upon that of the exterior tube; taking care that the circular hole is duly placed to the exterior one. These circular holes are not seen in fig. 18, being supposed in the opposite side, where the wheel is fixed. The adjustment for the focus is now only necessary; which is obtained by pushing downwards or upwards the proper tube, till the object appear quite distinct. In viewing transparent objects, the instrument may be used in two positions; one vertical, when the light is to be reflected upon the object by the mirror; the other, by looking up directly against the light of a candle, common light, &c.; in which case the mirror must be taken away. In viewing opaque objects, the mirror is not used; but as much common light as possible must be admitted through the circular holes in the sides of the tubes.
There is a spare hole in the transparent wheel, and also one in the opaque, to receive any occasional object that is to be viewed. Any sort of object whatsoever may be viewed, by only pushing up the microscope tube into its exterior, and bringing the first eye-tube to its focal distance from the object.
The brafs tubes are so contrived, that they stop when drawn out to the full length: so that by applying one hand to the outside tube, and the other to the end of the smallest tube, the telescope at one pull may be drawn out; then any of the tubes (that next to the eye is best) may be pushed in gradually, till the most distinct view of the object be obtained.
The tubes all slide through short brafs spring tubes, any of which may be unscrewed from the ends of the sliding tubes by means of the milled edges which project above the tubes, taken from each other, and the springs set clear if required.
III. Of Solar Microscopes.
This instrument, in its principle, is composed of a tube, a looking-glasf or mirror, a convex lens, and Wilson's single microscope before described. The sun's rays being reflected through the tube by means of the mirror upon the object, the image or picture of the object is thrown distinctly and beautifully upon a screen of white paper or a white linen sheet, placed at a proper distance to receive the same; and may be magnified
(A) Notwithstanding the properties that have been ascribed to the above instrument, and the praises bestowed upon it by some, which induced us to admit so minute a description, we must apprise our readers, that it has been omitted in Mr Adams's enumeration; and upon inquiry we learn, that it has fallen into neglect among the most judicious opticians, being found too imperfect to serve the purposes of science, and too complicated for the use of persons who seek only entertainment. Microscope magnified to a size not to be conceived by those who have not seen it; for the farther the screen is removed, the larger will the object appear; inasmuch, that a loupe may thus be magnified to the length of five or six feet, or even a great deal more; though it is more distinct when not enlarged to above half that size.
The different forms in which the Solar Microscope is constructed, are as follow:
1. The old construction is represented in fig. 21. A is a square wooden frame, through which paws two long screws affixed by a couple of nuts i, i. By these it is fastened firmly to a window shutter, wherein a hole is made for its reception; the two nuts being let into the shutter, and made fast thereto. A circular hole is made in the middle of this frame to receive the piece of wood B, of a circular figure; whose edge, that projects a little beyond the frame, composes a shallow groove 2, wherein runs a catgut 3; which, by twirling round, and then crossing over a brass pulley 4, (the handle whereof 5, passes through the frame), affords an easy motion for turning round the circular piece of wood B, with all the parts affixed to it. C is a brass tube, which, screwing into the middle of the circular piece of wood, becomes a case for the uncovered brass tube D to be drawn backwards or forwards in. E is a smaller tube, of about one inch in length, cemented to the end of the larger tube D. F is another brass tube, made to slide over the above described tube E; and to the end of this the microscope must be screwed, when we come to use it. G is a convex lens, whose focus is about 12 inches, designed to collect the sun's rays, and throw them more strongly upon the object. H is a looking-glass of an oblong figure, set in a wooden frame, fastened by hinges in the circular piece of wood B, and turning about therewith by means of the above-mentioned catgut. I is a jointed wire, partly brass and partly iron; the brass part, whereof 6, which is flat, being fastened to the mirror, and the iron part 7, which is round, passing through the wooden frame, enable the observer, by putting it backwards or forwards, to elevate or depress the mirror according to the sun's altitude. There is a brass ring at the end of the jointed wire 8, whereby to manage it with the greater ease. The extremities of the catgut are fastened to a brass pin, by turning of which it may be braced up, if at any time it becomes too slack.
When this microscope is employed, the room must be rendered as dark as possible; for on the darkness of the room, and the brightness of the sunshine, depend the sharpness and perfection of your image. Then putting the looking-glass G through the hole in your window shutter, fasten the square frame A to the shutter by its two screws and nuts i, i. This done, adjust your looking-glass to the elevation and situation of the sun, by means of the jointed wire H, together with the catgut and pulley, 3, 4. For the first of these raising or lowering the glass, and the other inclining it to either side, there results a twofold motion, which may easily be so managed as to bring the glass to a right position, that is, to make it reflect the sun's rays directly through the lens 5, upon the paper screen, and form thereon a spot of light exactly round. But though the obtaining a perfect circular spot of light upon the screen before you apply the microscope, is a certain proof that your mirror is adjusted right, that proof must not always be expected; for the sun is so low in winter, that if it shine in a direct line against the window, it cannot then afford a spot of light exactly round; but if it be on either side, a round spot may be obtained, even in December. As soon as this appears, screw the tube D into the brass collar provided for it in the middle of your wood work, taking care not to alter your looking-glass; then screwing the magnifier you choose to employ to the end of your microscope in the usual manner, take away the lens at the other end thereof, and place a slider containing the objects to be examined, between the thin brass plates, as in the other ways of using the microscope.
Things being thus prepared, screw the body of the microscope over the small end E of the brass tube F; which slip over the small end E of the tube D, and pull out the said tube D less or more as your object is capable of enduring the sun's heat. Dead objects may be brought within about an inch of the focus of the convex lens 5; but the distance must be shortened for living creatures, or they will soon be killed.
If the light fall not exactly right, you may easily, by a gentle motion of the jointed wire and pulley, direct it through the axis of the microscopic lens. The short tube F, to which the microscope is screwed, renders it easy, by sliding it backwards or forwards on the other tube E, to bring the objects to their focal distance; which will be known by the sharpness and clearness of their appearance: they may also be turned round by the same means without being in the least disordered.
The magnifiers most useful in the solar microscope are in general, the fourth, fifth, or sixth. The screen on which the representations of the objects are thrown, is usually composed of a sheet of the largest elephant paper, strained on a frame which slides up or down, or turns about at pleasure on a round wooden pillar, after the manner of some fire screens. Larger screens may also be made of several sheets of the same paper pasted together on cloth, and let down from the ceiling with a roller like a large map.
"This microscope (says Mr Baker) is the most entertaining of any; and perhaps the most capable of making discoveries in objects that are not too opaque: as it shows them much larger than can be done any other way. There are also several conveniences attending it, which no other microscope can have: for the weakest eyes may use it without the least straining or fatigue: numbers of people together may view any object at the same time; and by pointing to the particular parts thereof, and discovering what lies before them, may be able better to understand one another, and more likely to find out the truth, than in other microscopes, where they must peep one after another, and perhaps see the object neither in the same light nor in the same position. Those also, who have no skill in drawing, may, by this contrivance, easily sketch out the exact figure of any object they have a mind to preserve a picture of; since they need only fasten a paper on the screen, and trace it out thereon either with a pen or pencil, as it appears before them. It is worth Microscope, the while of those who are desirous of taking many draughts in this way, to get a frame, wherein a sheet of paper may be put in or taken out at pleasure; for if the paper be single, the image of an object will be seen almost as plainly on the back as on the fore side; and, by standing behind the screen, the shade of the hand will not obstruct the light in drawing, as it must in some degree when one stands before it." This construction, however, has now become rather obsolete, and is superseded by the following.
II. The improved Solar Microscope, as used with the improved single Microscope, with teeth and pinion. Fig. 22. represents the whole form of the single microscope; the parts of which are as follows: ABCD the external tube; GHIK the internal moveable one; QM part of another tube within the last, at one end of which is fixed a plate of brass hollowed in the middle, for receiving the glass tubes: there is also a moveable flat plate, between which, and the fixed end of the second tube, the ivory sliders are to be placed. L, a part of the microscope, containing a wire spiral spring, keeping the tube QM with its plates firm against the fixed part IK of the second tube.
EF is the small rack-work of teeth and pinion, by which the tube IG is moved gradually to or from the end AB, for adjusting the objects exactly to the focus of different lengths. NO is a brass slider, with five magnifiers; any one of which may easily be placed before the object. It is known when either of the glasses is in the centre of the eye-hole, by a small spring falling into a notch in the side of the slider, made against each of the glasses. Those parts of the apparatus, fig. 14, marked No 15, 16, 17, 18, 19, 20, 21, and 22, are made use of here to this microscope. GH is a brass cell, which holds an illuminating glass for converging the sun's beams or the light of a candle strongly upon the objects. The aperture of the glass is made greater or less, by two circular pieces of brass, with holes of different sizes, that are screwed separately over the said lens. But at times objects appear best when the microscope is held up to the common light only, without this glass. It is also taken away when the microscope is applied to the apparatus now to be described.
Fig. 23. represents the apparatus, with the single microscope screwed to it, which constitutes the Solar Microscope. AB is the inner moveable tube, to which the single microscope is screwed. CD is the external tube, containing a condensing convex glass at the end D, and is screwed into the plate EF, which is cut with teeth at its circumference, and moved by the pinion I, that is fixed with the plate GH. This plate is screwed fast against the window-shutter, or board fitted to a convenient window of a darkened room, when the instrument is used. KL is a long frame, fixed to the circular plate EF; containing a looking-glass or mirror for reflecting the solar rays through the lens in the body of the tube D. O is a brass milled head, fastened to a worm or endless screw; which on the outside turns a small wheel, by which the reflecting mirror M is moved upwards and downwards.
In using this microscope, the square frame GH is first to be screwed to the window shutter, and the room well darkened: which is best done by cutting a round hole of the size of the moveable plate EF, that carries the reflector, in the window-shutter, or Microscope board; and, by means of two brass nuts a, let into the shutter to receive the screws PP, when placed through the holes in the square frame GH, at the two holes QQ: which will firmly fasten the microscope to the shutter, and is easily taken away by only unscrewing the screws PP.
The white paper screen, or white cloth, to receive the images, is to be placed several feet distant from the window: which will make the representations the larger in proportion to the distance. The usual distances are from 6 to 16 feet.
The frame KL, with its mirror M, is to be moved by turning the pinion I, one way or the other, till the beams of the sun's light come through the hole into the room: then, by turning of the worm at O, the mirror must be raised or depressed till the rays become perfectly horizontal, and go straight across the room to the screen. The tube CD, with its lens at D, is now to be screwed into the hole of the circular plate EF: by this glass the rays will be converged to a focus; and from thence proceed diverging to the screen, and there make a large circle of light. The single microscope, fig. 22. is to be screwed on to the end AB (fig. 23.) of the inner tube; and the slider NO, with either of the lenses marked 1, 2, 3, 4, 5, or 6, in the centre of the hole at the end AB. This will occasion a circle of light upon the screen much larger than before. The slider or glass-tube, with the objects to be viewed, is to be placed between the plates at IK against the small magnifier, and moved at pleasure. By shifting the tube AB in or out, you may place the object in such a part of the condensed rays as shall be sufficient to illuminate it, and not scorch or burn it; which will generally require the glass to be about one inch distant from the focus. It now remains only to adjust the object, or to bring it so near to the magnifier that its image formed upon the screen shall be the most distinct or perfect: and it is effected by gently turning the pinion F, fig. 22. a small matter one way or the other. If the object be rather large in size, the least magnifiers are generally used, and vice versa.
No 1. is the greatest magnifier, and No 6. the least, in the brass slider NO. But, if desired, single lenses of greater magnifying powers are made: and they are applied, by being screwed to the end AB, fig. 22. and the brass slider NO is then taken away.
The same object may be variously magnified, by the lenses severally applied to it; and the degree of magnifying power is easily known by this rule: As the distance of the object is to that of its image from the magnifier; so is the length or breadth of the object to that of the image.
Instead of the brass sliders with the lenses NO, there is sometimes screwed a lens of a large size, and longer focal distance: the instrument is then converted into a magnifying telescope; and is adapted for viewing the larger kind of objects contained in large sliders, such as is represented at R. And, in the same manner, small objects of entertainment, painted upon glass like the sliders of a magic lantern, are much magnified, and represented upon the same screen.
The solar microscopes just described are capable only of magnifying transparent objects; for which pur- Microscope. The last instrument is extremely well adapted.
But as opaque objects form the most considerable part of the curious collections in the works of art as well as nature, a solar microscope for this purpose was a long time wanted.—For several years previous to 1774, Mr Martin made several essays towards the construction of such an instrument; and at last completed one about the time just mentioned, which he named,
III. The Opaque Solar Microscope. With this instrument (to use his own words) “all opaque objects, whether of the animal, vegetable, or mineral kingdom, may be exhibited in great perfection, in all their native beauty; the lights and shades, the prominences and cavities, and all the varieties of different hues, tints, and colours; heightened by reflection of the solar rays condensed upon them.—Transparent objects are also shown with greater perfection than by the common solar microscope.”
Fig. 24. represents the solar opaque microscope, mounted for exhibiting opaque objects.
Fig. 25. is the single tooth and pinion microscope, as before, which is used for showing transparent objects; the cylindrical tube Y thereof being made to fit into the tube FE of the solar microscope.
ABCDEF, (fig. 24.) represents the body of the solar microscope; one part thereof, ABCD, is conical; the other, CDEF, is cylindrical. The cylindrical part receives the tube G of the opaque box, or the tube Y of the single microscope. At the large end AB of the conical part, there is a lens to receive the rays from the mirror, and reflect them towards the box HIKL. NOP is a brass frame; which is fixed to the moveable circular plate a b c; in this frame there is a plane mirror, to reflect the solar rays on the aforementioned lens. This mirror may be moved into the most convenient position for reflecting the light, by means of the nuts Q and R. By the nut Q it may be moved from east to west; and it may be elevated or depressed by the nut R. de, Two screws to fasten the microscope to a window shutter. The box for opaque objects is represented at HIKL; it contains a plane mirror M, for reflecting the light which it receives from the large lens to the object, and thereby illuminating it; S is a screw to adjust this mirror, or place it at a proper angle for reflecting the light. VX, two tubes of brass, one sliding within the other, the exterior one in the box HIKL; these carry the magnifying lenses; the interior tube is sometimes taken out, and the exterior one is then used by itself. Part of this tube may be seen in the plate within the box HIKL. At H there is a brass plate, the back part of which is fixed to the hollow tube h, in which there is a spiral wire, which keeps the plate always bearing against the side H of the brass box HIKL. The sliders, with the opaque objects, pass between this plate and the side of the box; to put them there, the plate is to be drawn back by means of the nut g; i k is a door to one side of the opaque box. The foregoing pieces constitute the several parts necessary for viewing opaque objects. We shall now proceed to describe the single microscope, which is used for transparent objects; but in order to examine these, the box HIKL must be first removed, and in its place we must insert the tube Y of the single microscope that we are now going to describe.
Fig. 25. represents a large tooth and pinion microscope: at m, within the body of this microscope, are two thin plates, that are to be separated in order to let the ivory sliders pass between them; they are pressed together by a spiral spring, which bears up the under plate, and forces it against the upper one.
The slider S (under fig. 24.), which contains the magnifiers, fits into the hole n; and any of the magnifiers may be placed before the object, by moving the aforesaid slider: when the magnifier is at the centre of the hole P, a small spring falls into one of the notches which is on the side of the slider.
Under the plate m are placed two lenses, for enlarging the field of view on the screen: the smaller of the two is fixed in a piece of brass, and is nearest the plate m; this is to be taken out when the magnifiers, No 4, 5, or 6, are used, or when the megaloscope lens T (fig. 24.) is used; but is to be replaced for No 1, 2, 3.
This microscope is adjusted to the focus by turning the milled nut O.
To use the solar microscope:—Make a round hole in the window shutter, a little larger than the circle a b c; pass the mirror NOP through this hole, and apply the square plate to the shutter; then mark with a pencil the places which correspond to the two holes through which the screw is to pass; take away the microscope, and bore two holes at the marked places, sufficiently large to let the milled screws d e pass through them.
The screws are to pass from the outside of the shutter, to go through it; and being then screwed into their respective holes in the square plate, they will, when screwed home, hold it fast against the inside of the shutter, and thus support the microscope.
Screw the conical tube ABCD to the circle a b c, and then slide the tube G of the opaque box into the cylindrical part CDEF of the body, if opaque objects are to be examined; but if they be transparent objects you mean to show, then place the tube Y within the tube CDEF.
The room is to be darkened as much as possible, that no light may enter but what passes through the body of the microscope; for, on this circumstance, together with the brightness of the sunlight, the perfection and distinctness of the image in a great measure depend.
When the microscope is to be used for opaque objects, 1. Adjust the mirror NOP, so as to receive the solar rays, by means of the two finger screws or nuts, QR; the first, Q, turns the mirror to the right or left; the second, R, raises or depresses it: this you are to do till you have reflected the sun’s light through the lens at AB strongly upon a screen of white paper placed at some distance from the window, and formed thereon a round spot of light. An unexperienced observer will find it more convenient to obtain the light by forming this spot before he puts on either the opaque box or the tooth and pinion microscope.
Now put in the opaque box, and place the object between the plates at H; open the door i k, and adjust the mirror M till you have illuminated the object strongly. If you cannot effect this by the screw S, you must move the screws Q, R, in order to get the light reflected strongly from the mirror NOP, or the mirror Microscope-mirror M, without which the latter cannot illuminate the object.
The object being strongly illuminated, shut the door i k, and a distinct view of the object will soon be obtained on your screen, by adjusting the tubes V X, which is effected by moving them backwards or forwards.
A round spot of light cannot always be procured in northern latitudes, the altitude of the sun being often too low; neither can it be obtained when the sun is directly perpendicular to the front of the room.
As the sun is continually changing its place, it will be necessary, in order to keep his rays full upon the object, to keep them continually directed through the axis of the instrument, by the two screws Q and R.
To view transparent objects, remove the opaque box, and insert the tube Y, fig. 25, in its place; put the slider S into its place at n, and the slider with the objects between the plates at m; then adjust the mirror NOP, as before directed by the screws Q, R, so that the light may pass through the object; regulate the focus of the magnifier by the screw O. The most pleasing magnifiers in use are the fourth and fifth.
The size of the object may be increased or diminished, by altering the distance of the screen from the microscope; five or six feet is a convenient distance.
To examine transparent objects of a larger size, or to render the instrument what is usually called a megalacope, take out the slider S from its place at n, and screw the button T (fig. 24.) into the hole at P, fig. 25, and remove the glass which is under the plate at m, and regulate the light and focus agreeable to the foregoing directions.
N.B. At the end of the tube G there is a lens for increasing the density of the rays, for the purpose of burning or melting any combustible or fusible substance; this lens must be removed in most cases, lest the objects should be burnt. The intensity of the light is also varied by moving this tube backwards or forwards.
Apparatus of the Opaque Solar Microscope.—The large square plate and mirror; the body of the microscope; the opaque box and its tube; the tooth and pinion microscope; the slider with the magnifiers; the megalacope magnifier; the two screws d and e; some ivory sliders; some sliders with opaque objects; a brass frame, with a bottom of soft deal to stick any object on; a brass cylinder K (fig. 31.), for confining opaque objects.
IV. The CAMERA OBSCURA, or LUCERNAL, Microscope.
The great facility with which objects can be represented on paper or a rough glass in the camera obscura, and copies drawn from them by any person though unskilled in drawing, evidently suggested the application of the microscope to this instrument. The greatest number of experiments that appear to have been made with this view, were by Mr Martin and Mr Adams; the former of whom frequently applied the microscope to the portable camera, and with much effect and entertainment. But these instruments being found to answer only with the assistance of the sun, Mr Adams directed his experiments to the construction of an instrument of more extended utility, which could be equally employed in the daytime and by night. He accordingly succeeded so far as to produce, by candle-light, the images of objects refracted from a single magnifier upon one or two large convex leaves (of about five inches or upwards in diameter), at the end of a pyramidal shaped box, in a very pleasing and magnified appearance, so as to give opaque objects as well as transparent ones the utmost distinctness of representation; but still the light of a candle or lamp was found generally insufficient to throw the requisite degree of illumination upon the objects. The invention of what is called Argand's lamp, within these few years offered a complete remedy for this defect, by the intensity and steadiness of its light. This did not escape Mr Adams (son of the former), who immediately applied it; and who had likewise so altered and improved his father's instrument, both in construction and form, as to render it altogether a different one, and far more perfect and useful.
The advantages and properties of this excellently conceived instrument are numerous and important. As the far greater part of the objects which surround us are opaque (says our author), and very few are sufficiently transparent to be examined by the common microscopes, an instrument that could be readily applied to the examination of opaque objects has always been a desideratum. Even in the examination of transparent objects, many of the fine and more curious portions are lost, and drowned, as it were, in the light which must be transmitted through them; while different parts of the same object appear only as dark lines or spots, because they are so opaque as not to permit any light to pass through them. These difficulties, as well as many more, are obviated in the lucernal microscope; by which opaque objects of various sizes may be seen with ease and distinctness: the beautiful colours with which most of them are adorned, are rendered more brilliant, without changing in the least the real tint of the colour; and the concave and convex parts retain also their proper form.—The facility with which all opaque objects are applied to this instrument, is another considerable advantage, and almost peculiar to itself; as the texture and configuration of the more tender parts are often hurt by previous preparation, every object may be examined by this instrument, first as opaque, and afterwards (if the texture will admit of it) as transparent.—The lucernal microscope does not in the least fatigue the eye; the object appears like nature itself, giving ease to the sight and pleasure to the mind: there is also, in the use of this instrument, no occasion to that the eye which is not directed to the object. A further advantage peculiar to this microscope is, that by it the outlines of every object may be taken, even by those who are not accustomed to draw; while those who can draw well will receive great assistance, and execute their work with more accuracy and in less time than they would otherwise have been able to have performed it. Transparent objects as well as opaque may be copied in the same manner. The instrument may be used at any time of the day, but the best effect is by night; in which respect it has a superiority over the solar microscope, as that instrument can only be used when the sun shines.
Transparent objects may be examined with the lucernal microscope in three or four different modes, Microscope from a blaze of light almost too great for the eye to bear, to that which is perfectly easy to it: And by the addition of a tin lanthorn to the apparatus, may be thrown on a screen, and exhibited at one view to a large company, as by the solar microscope.
We shall now proceed to the description of the instrument and apparatus as given by Mr Adams.
Fig. 26. represents the improved Lucernal Microscope, mounted to view opaque objects. ABCD is a large mahogany pyramidal box, which forms the body of the microscope; it is supported firmly on the brass pillar FG, by means of the socket H and the curved piece IK.
LMN is a guide for the eye, in order to direct it in the axis of the lenses; it consists of two brass tubes, one sliding within the other, and a vertical flat piece, at the top of which is the hole for the eye. The outer tube is seen at MN, the vertical piece is represented at LM. The inner tube may be pulled out, or pushed in, to adjust it to the focus of the glasses. The vertical piece may be raised or depressed, that the hole, through which the object is to be viewed, may coincide with the centre in the field of view; it is fixed by a milled screw at M, which could not be shown in this figure.
At N is a dove-tailed piece of brass, made to receive the dove-tail at the end of the tubes MN, by which it is affixed to the wooden box ABCDE. The tubes MN may be removed from this box occasionally, for the convenience of packing it up in a less compass.
OP, a small tube which carries the magnifiers.
O, one of the magnifiers; it is screwed into the end of a tube, which slides within the tube P; the tube P may be unscrewed occasionally from the wooden body.
QRSTVX, a long square bar, which passes through the sockets YZ, and carries the stage or frame that holds the objects; this bar may be moved backward or forward, in order to adjust it to the focus by means of the pinion which is at a.
b, A handle furnished with an universal joint, for more conveniently turning the pinion. When the handle is removed, the nut (fig. 27.) may be used in its stead.
d, e, A brass bar, to support the curved piece KI, and keep the body AB firm and steady.
fg hi, The stage for opaque objects: it fits upon the bar QRST by means of the socket hi, and is brought nearer to or removed farther from the magnifying lens by turning the pinion a; the objects are placed in the front side of the stage (which cannot be seen in this figure) between four small brass plates; the edges of two of these are seen at k l. The two upper pieces of brass are moveable; they are fixed to a plate, which is acted on by a spiral spring, that presses them down, and confines the slider with the objects: this plate, and the two upper pieces of brass, are lifted up by the small nut m.
At the lower part of the stage, there is a semicircular lump of glass n, which is designed to receive the light from the lamp, fig. 29. and to collect and throw it on the concave mirror o, whence it is to be reflected on the object.
The upper part fg hs (fig. 26.) of the opaque stage takes out, that the stage for transparent objects may be inserted in its place.
Fig. 23. represents the stage for transparent objects; Fig. 28. the two legs 5 and 6 fit into the top of the under part r s h i of the stage for opaque objects; j is the part which confines or holds the sliders, and through which they are to be moved; g and 10 a brass tube, which contains the lenses for condensing the light, and throwing it upon the object; there is a second tube within that, marked g and 10, which may be placed at different distances from the object by the pin 11.
When this stage is used as a single microscope, without any reference to the lucernal, the magnifiers or object lenses, are to be screwed into the hole 12, and to be adjusted to a proper focus by the nut 13.
N.B. At the end AB (fig. 26.) of the wooden body there is a slider, which is represented as partly drawn out at A: when quite taken out, three grooves will be perceived; one of which contains a board that forms the end of the box; the next contains a frame with a grayed glass; and the third, or that farthest from the end AB, two large convex lenses.
Fig. 29. represents one of Argand's lamps, which are the most suitable for microscopic purposes, on account of the clearness, the intensity, and the steadiness of the light. The following account of the method of managing them, with other observations, is copied from an account given by Mr Parker with those he sells.
The principle on which the lamp acts, consists in disposing the wick in thin parts, so that the air may come into contact with all the burning fuel; by which means, together with an increase of the current of air occasioned by rarefaction in the glass tube, the whole of the fuel is converted into flame.
The wicks are circular; and, the more readily to regulate the quantity of light, are fixed on a brass collar, with a wire handle, by means of which they are raised or depressed at pleasure.
To fix the wick on, a wooden mandril is contrived, which is tapered at one end, and has a groove turned at the other.
The wick has a selvage at one end, which is to be put foremost on the mandril, and moved up to the groove; then putting the groove into the collar of the wick-holder, the wick is easily pushed forward upon it.
The wick-holder and wick being put quite down in their place, the spare part of the wick should, while dry, be set alight, and suffered to burn to the edge of the tubes; this will leave it more even than by cutting, and, being black by burning, will be much easier lighted: for this reason, the black should never be quite cut off.
The lamp should be filled an hour or two before it is wanted, that the cotton may imbibe the oil and draw the better.
The lamps which have a reservoir and valve, need no other direction for filling than to do it with a proper trimming pot, carefully observing when they are full; then pulling up the valve by the point, the reservoir, being turned with the other hand, may be replaced without spilling a drop.
Those lamps which fill in the front like a bird-fountain, must be reclined on the back to fill; and this should Microscope should be done gently, that the oil in the burner may return into the body when so placed and filled; if, by being too full, and oil appears above the guard, only move the lamp a little, and the oil will disappear; the lamp may then be placed erect, and the oil will flow to its proper level.
The oil must be of the spermaceti kind, commonly called chamber oil, which may generally be distinguished by its paleness, transparency, and inoffensive scent: all those oils which are of a red and brown colour, and of an offensive scent, should be carefully avoided, as their glutinous parts clog the lamp, and the impurities in such oil, not being inflammable, will accumulate and remain in the form of a crust on the wick. Seal oil is nearly as pale and sweet as chamber oil; but being of a heavy fluggith quality, is not proper for lamps with fine wicks.
Whenever bad oil has been used, on changing it, the wick must also be charged; because, after having imbibed the coarse particles in its capillary tubes, it will not draw up the fine oil.
To obtain the greatest degree of light, the wick should be trimmed exactly even, the flame will then be completely equal.
There will be a great advantage in keeping the lamp clean, especially the burner and air tubes; the neglect of cleanliness in lamps is too common: a candlestick is generally cleaned every time it is used, so should a lamp; and if a candlestick is not to be objected to because it does not give light after the candle is exhausted, so a lamp should not be thought ill of, if it does not give light when it wants oil or cotton: but this last has often happened, because the deficiency is less visible.
The glass tubes are best cleaned with a piece of wash leather.
If a fountain lamp is left partly filled with oil, it may be liable to overflow: this happens by the contraction of the air when cold, and its expansion by the warmth of a room, the rays of the sun, or the heat of the lamp when re-lighted: this accident may be effectually prevented by keeping the reservoir filled, the oil not being subject to expansion like air. On this account, those with a common reservoir are best adapted for microscopic purposes.
To examine Opaque Objects, with the Lucernal Microscope. To render the use of this instrument easy, it is usually packed with as many of the parts together as possible: it occupies on this account rather more room, but is much less embarrassing to the observer, who has only three parts to put on after it is taken out of its box, namely, the guide for the eye, the stage, and the tube with its magnifier.
But to be more particular: Take out the wooden slider A (fig. 26.), then lift out the cover and the gray glass, from their respective grooves under the slider A.
Put the end N of the guide for the eye LMN into its place, so that it may stand in the position which is represented in this figure.
Place the socket which is at the bottom of the opaque stage, on the bar QXT, so that the concave mirror o may be next the end DE of the wooden body.
Screw the tubes PO into the end DE. The magnifier you intend to use is to be screwed on the end O of these tubes.
The handle G b, or the milled nut, fig. 27., must be placed on the square end of the pinion a.
Place the lamp lighted before the glass lamp n, and the object you intend to examine between the spring plates of the stage; and the instrument is ready for use.
In all microscopes there are two circumstances which must be particularly attended to: first, the modification of the light, or the proper quantity to illuminate the object; secondly, the adjustment of the instrument to the focus of the glass and eye of the observer. In the use of the lucernal microscope there is a third circumstance, which is, the regulation of the guide for the eye.
1. To throw the light upon the object. The flame of the lamp is to be placed rather below the centre of the glass lamp n, and as near it as possible; the concave mirror o must be so inclined and turned as to receive the light from the glass lamp, and reflect it thence upon the object; the best situation of the concave mirror and the flame of the lamp depends on a combination of circumstances, which a little practice will discover.
2. To regulate the guide for the eye, or to place the centre of the eye-piece L so that it may coincide with the focal point of the lenses and the axis of vision: Lengthen and shorten the tubes MN, by drawing out or pushing in the inner tube, and raising or depressing the eye-piece ML, till you find the large lens (which is placed at the end AB of the wooden body) filled by an uniform field of light, without any prismatic colours round the edge; for till this piece is properly fixed, the circle of light will be very small, and only occupy a part of the lens; the eye must be kept at the centre of the eye-piece L, during the whole of the operation; which may be rendered somewhat easier to the observer, on the first use of the instrument, if he hold a piece of white paper parallel to the large lens, removing it from or bringing it nearer to them till he find the place where a lucid circle, which he will perceive on the paper, is brightest and most distinct; then he is to fix the centre of the eye-piece to coincide with that spot; after which a very small adjustment will set it perfectly right.
3. To adjust the lenses to their focal distance. This is effected by turning the pinion a, the eye being at the same time at the eye-piece L. The gray glass is often placed before the large lenses, while regulating the guide for the eye, and adjusting for the focal distance.
If the observer, in the process of his examination of an object, advance rapidly from a shallow to a deep magnifier, he will save himself some labour by pulling out the internal tube at O.
The upper part f g r s of the stage is to be raised or lowered occasionally, in order to make the centre of the object coincide with the centre of the lens at O.
To delineate objects, the gray glass must be placed before the large lenses; the picture of the object will be formed on this glass, and the outline may be accurately taken by going over the picture with a pencil. Microscope. The opaque part may be used in the daytime without a lamp, provided the large lenses at A.B are screened from the light.
To use the Lucernal Microscope in the examination of Transparent Objects.—The instrument is to remain as before: the upper part f.g.s of the opaque stage must be removed, and the stage for transparent objects, represented at fig. 28, put in its place; the end 9 to be next the lamp.
Place the grayed glass in its groove at the end A.B, and the objects in the slider-holder at the front of the stage; then transmit as strong a light as you are able on the object, which you will easily do by raising or lowering the lamp.
The object will be beautifully depicted on the gray glass; it must be regulated to the focus of the magnifier, by turning the pinion a.
The object may be viewed either with or without the guide for the eye. A single observer will see an object to the greatest advantage by using this guide, which is to be adjusted as we have described above. If two or three wish to examine the object at the same time, the guide for the eye must be laid aside.
Take the large lens out of the groove, and receive the image on the gray glass; in this case, the guide for the eye is of no use: if the gray glass be taken away, the image of the object may be received on a paper screen.
Take out the gray glass, replace the large lenses, and use the guide for the eye; attend to the foregoing directions, and adjust the object to its proper focus. You will then see the object in a blaze of light almost too great for the eye, a circumstance that will be found very useful in the examination of particular objects. The edges of the object in this mode will be somewhat coloured: but as it is only used in this full light for occasional purposes, it has been thought better to leave this small imperfection, than, by remedying it, to sacrifice greater advantages; the more so, as this fault is easily corrected, a new and interesting view of the object is obtained, by turning the instrument out of the direct rays of light, and permitting them to pass through only in an oblique direction, by which the upper surface is in some degree illuminated, and the object is seen partly as opaque, partly as transparent. It has been already observed, that the transparent objects might be placed between the slider-holders of the stage for opaque objects, and then be examined as if opaque.
Some transparent objects appear to the greatest advantage when the lens at 9 is taken away; as, by giving too great a quantity of light, it renders the edges less sharp.
The variety of views which may be taken of every object by means of the improved lucernal microscope, will be found to be of great use to an accurate observer: it will give him an opportunity of correcting or confirming his discoveries, and investigating those parts in one mode which are invisible in another.
To throw the image of transparent objects on a screen as in the solar microscope.—It has been long a microscopical desideratum, to have an instrument by which the image of transparent objects might be thrown on a screen, as in the common solar microscope; and this not only because the sun is so uncertain in this climate, and the use of the solar microscope requires confinement in the finest part of the day, when time seldom hangs heavy on the mind; but as it also affords an incrust of pleasure, by displaying its wonders to several persons at the same instant, without the least fatigue to the eye.
This purpose is now effectually answered, by affixing the transparent stage of the lucernal to a lanthorn, with one of Argand's lamps.—The lamp is placed within the lanthorn, and the end 9 to of the transparent stage is screwed into a female screw, which is riveted in the sliding part of the front of the lanthorn; the magnifying lenses are to be screwed into the hole represented at 12, and they are adjusted by turning the milled nut. The quantity of light is to be regulated by raising and lowering the sliding-plate or the lamp.
Apparatus which usually accompanies the improved Lucernal Microscope.—The stage for opaque objects, with its semicircular lump of glass, and concave mirror. The stage for transparent objects, which fits on the upper part of the foregoing stage. The sliding tube, to which the magnifiers are to be affixed; one end of these is to be screwed on the end D of the wooden body; the magnifier in use is to be screwed to the other end of the inner tube. Eight magnifying lenses: these are so constructed that they may be combined together, and thus produce a very great variety of magnifying powers. A fish-pan, such as is represented at I. A steel wire L, with a pair of nippers at one end, and a small cylinder of ivory at the other. A slider of brass N, containing a flat glass slider, and a brass slider into which are fitted some small concave glasses. A pair of forceps. Six large and six small ivory sliders, with transparent objects. Fourteen wooden sliders, with four opaque objects in each slider; and two spare sliders. Some capillary tubes for viewing small animalcula.
Ingenious men seldom content themselves with an instrument under one form; hence such a variety of microscopes, hence many alterations in the Lucernal Microscope. Mr Adams himself, we understand, has fitted up this last in a great many different ways; and it is reasonable to think that no person was more likely to give it every improvement of which it is susceptible. Of the alterations by other hands we shall only particularize one, made by Mr Jones of Holborn (B), whose description is as follows:
A (fig. 30.) represents a portion of the top of the ma-
(b) We trust the reader will never consider any paragraph wherein the name of an instrument-maker or other artist is inserted, as a recommendation of those artists by the editors of this work. In the course of a pretty extensive correspondence, they have been favoured with very liberal communications from various artists, for which they are greatly indebted to them; the inserting their names in this work is therefore to be considered Microscope hogany box in which it packs, to preserve it steady; it slides in a dove-tail groove withinside, a similar groove to which is cut in the top of the box A; so that when the instrument is to be used, it slips out of the box withinside, and then slips into the groove at top ready for use, almost instantly, as shown in the figure. The adjustment of the objects is at the stage E; for the right focal distance is readily and conveniently made by turning the long screw-rod BB, which goes through the two pillars supporting the box, and works in the base of the brass stage E; which base is also dovetailed, so as to have a regular and steady motion in another brass basis that supports it. In this instrument, therefore, the pyramidal box does not move; but the stage part only, which, from its small weight, moves in the most agreeable and steady manner. While observing the image of the object upon the glass through the sight-hole at G, the object may be moved or changed by only turning the rack-work and pinion applied to the stage by means of the handle D, for that purpose. By this contrivance you have no occasion to change your position during the view of the objects upon one of the sliders. This motion changes the objects horizontally only; and as they are generally placed exactly in one line, it answers all the purposes for which this motion is intended very well. But it may sometimes happen that the observer would wish to alter the vertical position of the object; to perform which there is another plain rod at F, that acts simply as a lever for this purpose, and moves the sliding part of the stage E vertically either upwards or downwards.
Thus, without altering his position, the observer may investigate all parts of the objects in the most satisfactory manner. Rack-work and pinion might be applied to the stage for the vertical motion also; but as it would materially enhance the expense, it is seldom applied. The brass work at the handle of D contains a Hooke's universal joint.
The brilliancy of the images of the objects shown upon the large lenses at the end of the box, being very frequently so great as to dazzle the eyes, Mr Jones applies a slight tinge of blue, green, and other coloured glasses, to the sight-hole at G, which softens this glare, and casts an agreeable hue upon the objects.
Description of those Parts of a Microscopical Apparatus, common to most Instruments, which are delineated at fig. 31.
A and B represent the brass cells which contain the magnifiers belonging to the different kinds of compound microscopes. The magnifiers are sometimes contained in a slider like that which is delineated at S (fig. 24). The lenses of A and B are confined by a small cap; on unscrewing this, the small lens may be taken out and cleaned. The magnifiers A of the external microscope are so contrived, that any two of them may be screwed together, by which means a considerable variety of magnifying powers is obtained.
To get at the lenses in the slider S (fig. 24.) take out the two screws which hold on the cover.
C represents the general form of the slider-holder. It consists of a cylindrical tube, in which an inner tube is forced up by a spring. It is used to receive the ivory or any other slider, in which the transparent objects are placed; these are to be slid between the two upper plates: the hollow part in one of the plates is designed for the glass tubes.
D, the condensing lens and its tube, which fits into the slider-holder C, and may be moved up and down in it. When this piece is pushed up as far as it will go, it condenses the light of a candle, which is reflected on it by the plain mirror of the compound microscope, and spreads it uniformly over the object; in this case it is best adapted to the shallowest magnifiers. If the deeper lenses are used, it should be drawn down, or rather removed further from the object, that it may concentrate the light in a small compass, and thus render it more dense. The condensing lens is sometimes fitted up differently; but the principle being the same, it will be easy to apply it to use notwithstanding some variations in the mechanism.
E, a brass cone. It fixes under the slider-holder, and is used to lessen occasionally the quantity of light which comes from the mirror to any object.
F, a box with two flat glasses, which may be placed at different distances from each other in order to confine a small living insect.
G, a small brass box to hold the silver speculum H.
H, a small silver concave speculum, designed to reflect the light from the mirror on opaque objects; it should only be used with the shallowest magnifiers. It is applied in different ways to the compound microscope; sometimes to a tube similar to that represented at X, which slides on the lower part of the body; sometimes it is screwed into the ring of the piece Q; the pin of this generally fits into one of the holes in the stage. When this speculum is used, the slider-holder should be removed.
I, a fish-pan, whereon a small fish may be fastened, in order to view the circulation of the blood: its tail is to be spread across the oblong hole at the smallest end, and tied fast by means of the ribbon fixed thereto, by shoving the knob which is on the back of it through the slit made in the stage; the tail of the fish may be brought under the lens which is in use.
K, a cylindrical piece, intended for the solar opaque microscope; by pulling back the spiral spring, smaller or larger objects may be confined in it.
L, a pair of triangular nippers for taking hold of and confining a large object.
M, a long steel wire, with a small pair of pliers at one end and a steel point at the other: the wire slips backwards or forwards in a spring tube, which is affixed to a joint, at the bottom of which is a pin to fit one
as a grateful acknowledgment from the editors for favours conferred on them,—not as a testimonial of their opinion of the abilities of an individual, or as designed to infuse any preference over others in the same line, where such preference has not been already bestowed by the public. one of the holes in the stage; this piece is used to confine small objects.
A small ivory cylinder that fits on the pointed end of the steel wire L; it is designed to receive opaque objects. Light-colored ones are to be stuck on the dark side, and vice versa.
M, a convex lens, which fits to the stage by means of the long pin adhering to it. This piece is designed to collect the light from the sun or a candle, and to throw them on any object placed on the stage; but it is very little used at present.
N, a brass slider, into which is fitted a flat piece of glass, and a brass slider containing four small glasses, one or two of them concave, the others flat; it is designed to confine small living objects, and when used is to be placed between the two upper plates of the slider-holder.
O, a glass tube to receive a small fish, &c.
P, represents one of the ivory sliders, wherein objects are placed between two pieces of talc, and confined by a brass ring.
Q, a piece to hold the speculum H: this piece is generally fitted to the microscope represented at fig. 12.
R, a pair of forceps, to take up any occasional object.
S, a camel's hair pencil to brush the dust off the glasses; the upper part of the quill is scooped out, to take up a drop of any fluid, and place it on either of the glasses for examination.
T, an instrument for cutting thin transverse sections of wood. It consists of a wooden base, which supports four brass pillars; on the top of the pillars is placed a flat piece of brass, near the middle of which there is a triangular hole.
A sharp knife, which moves in a diagonal direction, is fixed on the upper side of the above-mentioned plate, and in such a manner that the edge always coincides with the surface thereof.
The knife is moved backwards and forwards by means of the handle a. The piece of wood is placed in the triangular trough which is under the brass plate, and is to be kept steady therein by a milled screw which is fitted to the trough; the wood is to be pressed forward for cutting by the micrometer screw b.
The pieces of wood should be applied to this instrument immediately on being taken out of the ground, or else they should be soaked for some time in water, to soften them so that they may not hurt the edge of the knife.
When the edge of the knife is brought in contact with the piece of wood, a small quantity of spirits of wine should be poured on the surface of the wood, to prevent its curling up; it will also make it adhere to the knife, from which it may be removed by pressing a piece of blotting paper on it.
y, An appendage to the cutting engine, which is to be used instead of the micrometer foresight, being preferred to it by some. It is placed over the triangular hole, and kept flat down upon the surface of the brass plate, while the piece of wood is pressed against a circular piece of brass which is on the under side of it. This circular piece of brass is fixed to the foresight, by which its distance from the flat plate on which the knife moves may be regulated.
An ivory box, containing at one end spare talc microscope for the ivory sliders, and at the other spare rings for preserving the tales together and confining them to the slider.
Fluid microscopes have been also proposed; the first, it would appear, was suggested by Mr Grey. This was formed of water, and an account of it will be found in No. 221, 223, Phil. Trans. An improved microscope, on a similar principle, has been invented by Mr Brewster, of which the following is a description, taken from a note by the translator of Haüy's Natural Philosophy.
"A vertical bar (says Mr Gregory), is fixed upon a horizontal pedestal; and from the top of this bar proceeds a horizontal arm, which supports a circular case containing the lenses; below this another horizontal arm slides up and down, capable of adjustment by means of a screw, and carrying the usual sliders to hold the object which it is proposed to examine; and upon the pedestal is fixed the frame of a mirror, which has both an inclined and a horizontal motion, in order to illuminate any object upon the slider. The upper circular case is hollow, and contains four or more plano-convex lenses, which are constituted each of a drop of very pure and viscous turpentine varnish, taken up by the point of a piece of wood, and dropped upon a piece of very thin and well polished glass. The lenses thus formed may be made of any focal length by taking up a greater or a less quantity of fluid. The lower surface of the glass having been first smoked with a candle, the black pigment immediately below the lenses is then to be removed, so that no light may pass but through the lenses. The piece of glass is then perforated at its centre, and surrounded by a toothed wheel, which, when the wheel is put in the upper circular case, may be turned by a common endless screw, so that the fluid lenses shall be brought severally under an eye-aperture properly disposed, and any object be successfully examined with a variety of magnifying powers." Note, p. 365. See also Ferguson's Lectures by Brewster, vol. ii.
After what has been related of microscopes, they cannot be said to be complete without the valuable addition of a micrometer; for the use and advantages of which, see the article MICROMETER.
Having presented our readers with descriptions of the various microscopes generally used, we think it our duty to point out to them those which we conceive to be best calculated to answer the purposes of science. The first which presents itself to our mind is that of Ellis: It is better adapted than any other portable microscope, to the purpose of general observation; simple in its construction, and general in its application. To those who prefer a double microscope, we should recommend that figured in Plate CCXXXVIII, fig. 12. If opaque objects, as insects, &c., be subjects of investigation, the Lucernal Microscope claims the preference; but if amusement alone guides the choice, the Solar Microscope must be fixed upon.
We shall now proceed to explain some necessary particulars respecting the method of using microscopes; after which, we shall subjoin an enumeration of the principal On this subject Mr Adams, in his Essay on the Microscope, has been very copious; with a view, as he informs us, to remove the common complaint made by Mr Baker, "that many of those who purchase microscopes are so little acquainted with their general and extensive usefulness, and so much at a loss for objects to examine by them, that after diverting their friends some few times with what they find in the slides which generally accompany the instrument, or perhaps with two or three common objects, the microscope is laid aside as of little further value: whereas no instrument has yet appeared in the world capable of affording so constant, various, and satisfactory an entertainment to the mind."
I. In using the microscope, there are three things necessary to be considered. (1.) The preparation and adjustment of the instrument itself. (2.) The proper quantity of light, and the best method of directing it to the object. (3.) The method of preparing the objects, so that their texture may be properly understood.
1. With regard to the microscope itself, the first thing necessary to be examined is, whether the glasses be clean or not: if they are not so, they must be wiped with a piece of soft leather, taking care not to soil them afterwards with the fingers; and, in replacing them, care must be taken not to place them in an oblique situation. We must likewise be careful not to let the breath fall upon the glasses, nor to hold that part of the body of the instrument where the glasses are placed with a warm hand; because thus the moisture expelled by the heat from the metal will condense upon the glass, and prevent the object from being distinctly seen. The object should be brought as near the centre of the field of view as possible; for there only it will be exhibited in the greatest perfection. The eye should be moved up and down from the eye glass, of a compound microscope, till the situation is found where the largest field and most distinct view of the object are to be had; but every person ought to adjust the microscope to his own eye, and not to depend upon the situation it was placed in by another. A small magnifying power should always be begun with; by which means the observer will best obtain an exact idea of the situation and connexion of the whole; and will of consequence be less liable to form any erroneous opinion when the parts are viewed separately by a lens of greater power. Objects should also be examined first in their most natural position: for if this be not attended to, we shall be apt to form very inadequate ideas of the structure of the whole, as well as of the connexion and use of the parts. A living animal ought to be as little hurt or discomposed as possible.
From viewing an object properly, we may acquire a knowledge of its nature: but this cannot be done without an extensive knowledge of the subject, much patience, and many experiments; as in a great number of cases the images will resemble each other, though derived from very different substances. Mr Baker therefore advises us not to form an opinion too suddenly after viewing a microscopical object; nor to draw our inferences till after repeated experiments and examinations of the object in many different lights and positions; to pass no judgment upon things extended by force, or contracted by dryness, or in any manner out of a natural state, without making suitable allowances. The true colour of objects cannot be properly determined by very great magnifiers; for as the pores and interstices of an object are enlarged according to the magnifying power of the glasses made use of, the component particles of its substance will appear separated many thousand times farther asunder than they do to the naked eye: hence the reflection of the light from these particles will be very different, and exhibit different colours. It is likewise somewhat difficult to observe opaque objects; and as the apertures of the larger magnifiers are but small, they are not proper for the purpose. If an object be so very opaque, that no light will pass through it, as much as possible must be thrown upon the upper surface of it. Some consideration is likewise necessary in forming a judgment of the motion of living creatures, or even of fluids, when seen through the microscope; for as the moving body, and the space wherein it moves, are magnified, the motion will also be increased.
2. On the management of the light depends in a great measure the distinctness of the vision: and as, in order to have this in the greatest perfection, we must adapt the quantity of light to the nature of the object and the focus of the magnifier, it is therefore necessary to view it in various degrees of light. In some objects, it is difficult to distinguish between a prominence and a depression, a shadow or a black stain: or between a reflection of light and whiteness, which is particularly observable in the eye of the belladonna and other flies: all of these appearing very different in one position from what they do in another. The brightness of an object likewise depends on the quantity of light, the distinctness of vision, and on regulating the quantity to the object; for some will be in a manner lost in a quantity of light scarcely sufficient to render another visible.
There are various ways in which a strong light may be thrown upon objects; as by means of the sun and a convex lens. For this purpose, the microscope is to be placed about three feet from a southern window; then take a deep convex lens, mounted on a semicircle and stand, so that its position may easily be varied: place this lens between the object and the window, so that it may collect a considerable number of solar rays, and refract them on the object or the mirror of the microscope. If the light thus collected from the sun be too powerful, it may be lessened by placing a piece of oiled paper, or a piece of glass lightly grayed, between the object and lens. Thus a proper degree of light may be obtained, and diffused equally all over the surface of an object: a circumstance which ought to be particularly attended to; for if the light be thrown irregularly upon it, no distinct view can be obtained. If we mean to make use of the solar light, it will be found convenient to darken the room, and to reflect the rays of the sun on the above-mentioned lens by means of the mirror of a solar microscope fixed to the window-shutter: for thus the observer will be enabled to preserve the light on his subject, notwithstanding the motion of the sun. But by reason of this motion, and the variable state of the atmosphere, solar observations are rendered both tedious and inconvenient; whence it will be proper for the observer to be furnished with a large tin lanthorn, formed something like the common magic lanthorn, capable of containing one of Argand's lamps. This, however, ought not to be of the fountain kind, lest the rarefaction of the air in the lanthorn should force the oil over. There ought to be an aperture in the front of the lanthorn, which may be moved up and down, and be capable of holding a lens; by which means a pleasant and uniform as well as strong light may easily be procured. The lamp should likewise move on a rod, so that it may be easily raised or depressed. This lanthorn may likewise be used for many other purposes; as viewing of pictures, exhibiting microscopic objects on a screen, &c. A weak light, however, is best for viewing many transparent objects; among which we may reckon the prepared eyes of flies, as well as the animalcules in fluids. The quantity of light from a lamp or candle may be lessened by removing the microscope to a greater distance from them, or by diminishing the strength of the light which falls upon the objects. This may very conveniently be done by pieces of black paper with circular apertures of different sizes, and placing a larger or smaller one upon the reflecting mirror, as occasion may require. There is an oblique situation of the mirrors, which makes likewise an oblique reflection of the light easily discovered by practice, (but for which no general rule can be given in theory); and which will exhibit an object more distinctly than any other position, showing the surface, as well as those parts through which the light is transmitted. The light of a lamp or candle is generally better for viewing microscopic objects than day light; it being more easy to modify the former than the latter, and to throw it upon the objects with different degrees of density.
3. Swammerdam has excelled in the preparation of objects almost all other investigators. Neither difficulty nor disappointment could make him abandon the pursuit of any object until he had obtained a satisfactory idea of it. But unhappily, the methods he used in preparing his objects for the microscope are now entirely unknown. Boerhaave examined with the strictest attention all the letters and manuscripts of Swammerdam which he could find; but his researches were far from being successful. The following are all the particulars, which have thus come to the knowledge of the public.
For dissecting small insects, Swammerdam had a bras table made by S. Mutchenbroeck, to which were affixed two bras arms moveable at pleasure to any part of it. The upper part of these vertical arms was constructed in such a manner as to have a slow vertical motion; by which means the operator could readily alter their height as he saw convenient. One of these arms was to hold the minute objects, and the other to apply the microscope.
The lenses of Swammerdam's microscopes were of various sizes as well as foci; but all of them the best that could be procured, both for the transparency of the glass and the fineness of the workmanship. His observations were always begun with the smallest magnifiers, from which he proceeded to the greatest; but in the use of them, he was so exceedingly dexterous, that he made every observation subservient to that which succeeded it, and all of them to the confirmation of each other, and to the completing of the description. His chief art seems to have been in constructing leis- fans of an exquisite fineness, and making them very sharp. Thus he was enabled to cut very minute objects to much more advantage than could be done by knives and lancets; for these, though ever so sharp and fine, are apt to disorder delicate substances by displacing some of the filaments, and drawing them after them as they pass through the bodies; but the scissors cut them all equally. The knives, lancets, and styles he made use of in his dissections, were so fine that he could not see to sharpen them without the assistance of a magnifying glass; but with these he could dissect the intestines of bees with the same accuracy that the best anatomists can do those of large animals. He made use also of very small glass tubes no thicker than a bristle, and drawn to a very fine point at one end, but thicker at the other. These were for the purpose of blowing up, and thus rendering visible the smallest vessels which could be discovered by the microscope; to trace their courses and communications, or sometimes to inject them with coloured liquids.
Swammerdam sometimes made use of spirit of wine, water, or oil of turpentine, for suffocating the insects wished to examine; and would preserve them for a time in these liquids. Thus he kept the parts from putrefying, and gave them besides such additional strength and firmness, as rendered the dissections much more easy than they would otherwise have been. Having then divided the body transversely with the scissors, and made what observations he could without farther dissection, he proceeded to extract the intestines carefully with very fine instruments, to wash away the fat in the like careful manner; and thus to put the parts into such a state as would best expose them to view; but these operations are best performed while the insects are in their nymph stage.
Sometimes the delicate viscera of the insects, after having been suffocated as above mentioned, were put into water; after which, having shaken them gently, he procured an opportunity of examining them, especially the air vessels, which last he could thus separate entire from all the other parts, to the admiration of all who beheld them: as these vessels cannot be distinctly seen in any other manner, or indeed in any way whatever, without injuring them. Frequently also he injected water with a syringe to cleanse the parts thoroughly, after which he blew them up with air and dried them; thus rendering them durable, and fit for examination at a proper opportunity. Sometimes he made very important discoveries, by examining insects which he had preserved for several years in balsam. Other insects he punctured with a very fine needle; and after squeezing out all their moisture through the holes made in this manner, he filled them with air, by means of very slender glass tubes; then dried them in the shade; and lastly, anointed them with oil of spike in which a little rosin had been dissolved; and by which means they for a long time retained their proper forms. He was likewise in possession of a singular secret, by which he could preserve the limbs of insects as limber and perspicuous as ever they had been. He used to make a small puncture or incision in the tails of worms; Microscope. worms; and after having with great caution squeezed out all the humours, as well as great part of the visceræ, he injected them with wax in such a manner as to give them the appearance of living creatures in perfect health. He found that the fat of all insects was entirely dissolvable in oil of turpentine; by which means he was enabled plainly to discern the viscera; though, after this dissolution, it was necessary to cleanse and wash them frequently in clean water. In this manner he would frequently have spent whole days in the preparation of a single caterpillar, and cleansing it from its fat, in order to discover the true situation of the insect's heart. He had a singular dexterity in stripping off the skins of caterpillars that were on the point of spinning their cocoons. This was done by letting them drop by their threads into scalding water, and then suddenly withdrawing them. Thus the epidermis peeled off very easily; and, when this was done, he put them into distilled vinegar and spirit of wine mixed together in equal proportions; which, by giving a due degree of firmness to the parts, gave him an opportunity of separating them with very little trouble from the exuvia, without any danger to the internal parts. Thus the nymph could be shown to be wrapped up in the caterpillar and the butterfly in the nympha; and there is little doubt that those who look into the works of Swammerdam, will be amply recompensed, whether they consider the unexampled labour or the piety of the author.
M. Lyonet, an eminent naturalist, usually drowned the insects he designed to examine; by which means he was enabled to preserve both the freshness and transparency of the parts. According to him, the insect, if very small, viz. one-tenth of an inch, or little more, in length, should be dissected on a glass somewhat concave. If it should be suspected that the insect will putrefy by keeping for a few days, spirit of wine diluted with water must be substituted instead of pure water. The insect must be suffered to dry; after which it may be fastened by a piece of soft wax, and again covered with water.—Larger objects should be placed in a trough of thin wood; and for this purpose the bottom of a common chip box will answer very well; only surrounding the edge of it with soft wax, to keep in the water or other fluid employed in preserving the insect. The body is then to be opened; and if the parts are soft like those of a caterpillar, they should be turned back, and fixed to the trough by small pins, which ought to be set by a small pair of nippers. At the same time, the skin being stretched by another pair of finer forceps, the insect must be put into water, and dissected therein, occasionally covering it with spirit of wine. Thus the subject will be preserved in perfection, so that its parts may be gradually unfolded, no other change being perceived than that the soft elastic parts become stiff and opaque, while some others lose their colour.
The following instruments were made use of by M. Lyonet in his dissection of the Chenille de Saule. A pair of scissors as small as could be made, with long and fine arms: A pair of forceps, with their ends so nicely adjusted, that they could easily lay hold of a spider's thread, or a grain of sand: Two fine steel needles fixed in wooden handles, about two inches and three quarters in length; which were the most generally useful instruments he employed.
Dr Hooke, who likewise made many microscopic observations, takes notice, that the common ant or pilimire is much more troublesome to draw than other insects, as it is extremely difficult to get the body in a quiet natural posture. If its feet be fettered with wax or glue, while the animal remains alive, it so twists its body, that there is no possibility of gaining a proper view of it; and if it be killed before any observation is made, the shape is often spoiled before it can be examined. The bodies of many minute insects, when their life is destroyed, instantly shrivel up; and this is observable even in plants as well as insects, the surface of these small bodies being affected by the least change of air; which is particularly the case with the ant. If this creature, however, be dropped into rectified spirit of wine, it will instantly be killed; and when it is taken out, the spirit of wine evaporates, leaving the animal dry, and in its natural posture, or at least in such a state, that it may easily be placed in whatever posture we please.
Parts of Insects. The wings, in many insects, are so transparent, that they require no previous preparation: but some of those that are folded up under elytra or cases, require a considerable share of dexterity to unfold them; for these wings are naturally endowed with such a spring, that they immediately fold themselves again, unless care be taken to prevent them. The wing of the earwig, when expanded, is of a tolerable size, yet is folded up under a case not one-eighth part of its bulk; and the texture of this wing renders it difficult to be unfolded. This is done with the least trouble immediately after the insect is killed. Holding then the creature by the thorax, between the finger and the thumb, with a blunt pointed pin endeavour gently to open it, by spreading it over the forefinger, and at the same time gradually sliding the thumb over it. When the wing is sufficiently expanded, separate it from the insect by a sharp knife or a pair of scissors. The wing should be pressed for some time between the thumb and finger before it be removed; it should then be placed between two pieces of paper, and again pressed for at least an hour; after which time, as there will be no danger of its folding up any more, it may be put between the tales, and applied to the microscope. Similar care is requisite in displaying the wings of the notoneeta and other water insects, as well as most kinds of grylli.
The minute scales or feathers, which cover the wings of moths or butterflies, afford very beautiful objects for the microscope. Those from one part of the wing frequently differ in shape from such as are taken from other parts; and near the thorax, shoulder, and on the fringes of the wings, we generally meet with hair instead of scales. The whole may be brushed off the wing, upon a piece of paper, by means of a camel's hair pencil; after which the hairs can be separated with the assistance of a common magnifying glass.
It is likewise a matter of considerable difficulty to dissect properly the proboscis of insects, such as the guat, tabanus, &c. and the experiment must be repeated a great number of times before the structure and situation Microscope. tuation of the parts can be thoroughly investigated, as the observer will frequently discover in one what he could not in another. The collector of the bee, which forms a very curious object, ought to be first carefully washed in spirit of turpentine; by which means it will be freed from the unctuous matter adhering to it: when dry, it is again to be washed with a camel's hair pencil to dilengage and bring forward the small hairs which form part of this microscopic beauty. The best method of managing the stings of insects, which are in danger of being broken by reason of their hardness, is to soak the case and the rest of the apparatus for some time in spirit of wine or turpentine; then lay them on a piece of paper, and with a blunt knife draw out the sting, holding the sheath with the nail of the finger or any blunt instrument; but great care is necessary to pre- serve the feelers, which when cleaned add much to the beauty of the object. The beard of the lepus anaticeps is to be soaked in clean soft water, frequently brushing it while wet with a camel's hair pencil: after it is dried, the brushing must be repeated with a dry pencil to dilengage and separate the hairs, which are apt to adhere together.
To view to advantage the fat, brains, and other simi- lar substances, Dr Hooke advises to render the sur- face smooth, by pressing it between two plates of thin glass, by which means the matter will be rendered much thinner and more transparent; without this pre- caution, it appears confused, by reason of the parts lying too thick upon one another. For muscular fibres, take a piece of the flesh, thin and dry; moisten it with warm water, and after this is evaporated the vessels will appear more plain and distinct; and by repeated macerations they appear still more so. The exuviae of insects afford a pleasing object, and require but little preparation. If bent or curled up, they will become so relaxed by being kept a few hours in a moist atmosphere, that you may easily extend them to their natural posi- tions; or the steam of warm water will answer the purpose very well.
The eyes of insects in general form very curious and beautiful objects. Those of the libellula and other flies, as well as of the lobster, &c., must first be cleaned from the blood, &c., after which they should be soaked in water for some days; one or two skins are then to be separated from the eye, which would be otherwise too opaque and confused; but some care is requisite in this operation; for if the skin be rendered too thin, it is impossible to form a proper idea of the organization of the part. In some substances, however, the organiza- tion is such, that by altering the texture of the part, we destroy the objects which we wish to observe. Of this sort are the nerves, tendons, mucular fibres, many of which are viewed to most advantage when floating in some transparent fluid. Thus very few of the mucular fibres can be discovered when we attempt to view them in the open air, though great numbers may be seen if they be placed in water or oil. By viewing the thread of a ligament in this manner, we find it com- posed of a vast number of smooth round threads lying close together. Elastic objects should be pulled or stretched out while they are under the microscope, that the texture and nature of those parts, the figure of which is altered by being thus pulled out, may be more fully discovered.
Other objects. To examine bones by the microscope, Micro- scope, they should first be viewed as opaque objects; but af- terwards, by procuring thin slices of them, they may be viewed as transparent. The sections should be cut in all directions, and be well washed and cleaned; and in some cases maceration will be useful, or the bones may be heated red hot in a clear fire, and then taken out; by which means the bony cells will appear more conspicuous. The pores of the skin may be examined by cutting off a thin slice off the upper skin with a razor, and then a second from the same place; applying the latter to the microscope. The lizard, guana, &c., have two skins, one very transparent, the other thicker and more opaque; and, separating these two, you obtain very beautiful objects.
To view the scales of fish to advantage, they ought to be soaked in water for a few days, and then care- fully rubbed to clean them from the skin and dirt which may adhere to them. The scales of the eel are a great curiosity; and the more so, as this creature was not known to have any scales till they were disco- vered by the microscope. The method of discovering them is this. Take a piece of the skin of an eel from off its side, and spread it while moist on a piece of glass, that it may dry very smooth: when thus dried, the surface will appear all over dimpled or pitted by the scales, which lie under a sort of cuticle or thin skin; which may be raised with the sharp point of a penknife, together with the scales, which will then easily slip out; and thus we may procure as many as we please.
The leaves of many trees, as well as of some plants, when dissected, form a very agreeable object. In or- der to dissect them, take a few of the most perfect leaves you can find, and place them in a pan with clean water. Let them remain there three weeks, or a month, without changing the water: then take them up; and if they feel very soft, and almost rotten, they are sufficiently soaked. They must then be laid on a flat board, and holding them by the stalk, draw the edge of a knife over the upper side of the leaf, which will take off most of the skin. Then turn the leaf, and do the same with the under side; and when the skin is taken off on both sides, wash out the pulpy matter, and the fibres will be exhibited in a very beautiful man- ner. The leaf may be slit into two parts, by splitting the stalk; and the skins peeled from the fibres will also make a good object. This operation is best performed in the autumn; the fibres of the leaves are much stronger at that season, and less liable to be broken.—The internal structure of shells may be ob- served by grinding them down on a hone; and all ores and minerals should be carefully washed and brushed with a small brush, to remove any foreign matter that may adhere to them.
To view the circulation of the blood, we must ob- serve living animals of the most transparent kind.— A small eel is sometimes used for this purpose; in which case it must be cleansed from the slime naturally adhering to it; after which it may be put into a tube filled with water, where it can be viewed in a satis- factory manner. The tail of any other small fish may be viewed in the same manner, or put upon a slip of flat glass, and thus laid before the microscope. By filling the tube with water when an eel is made use of, we prevent in a great measure the sliminess of the animal from foiling the glass.
The particles of the blood form a very curious object, and have been carefully viewed by different philosophers; who, nevertheless, differ from one another very much in their accounts of them. The best method of viewing these is to take a small drop of blood when warm, and spread it as thin as possible upon a flat piece of glass. By diluting it a little with warm water, some of the large globules will be separated from the smaller, and many of them subdivided; or a small drop of blood may be put into a capillary glass tube, and then placed before the microscope. Mr Baker advises warm milk as proper to be mixed with the blood; but Mr Hewson diluted the blood with its own serum; and by this method he could preserve the small particles entire, and view them distinctly; and thus he found that they were not globular, as had been imagined by other anatomists, but flat. Having flaked a piece of the crafamentum of the blood in serum till the latter became a little coloured, he spread it with a soft hair pencil on a piece of thin glass, which he placed under the microscope, in such a manner as not to be quite horizontal, but rather higher at one end than the other. Thus the serum flows from the higher to the lower part; and, as it flows, some of the particles will be found to swim on their flat sides, and will appear to have a dark spot in the middle; while others will turn over from one side to the other as they roll down the glass. Many cruel experiments have been tried in order to observe the circulation of the blood in living creatures, and an apparatus had been invented for viewing the circulation in the mesentery of a frog; but as this can answer no useful purpose, and will never be put in practice by persons of humanity, we forbear to mention it.
II. Besides the objects for the microscope already mentioned, there are innumerable others, some hardly visible, and others totally invisible, to the naked eye; and which therefore, in a more peculiar sense, are denominated
Microscopic Animals. They are the animalcules or moving bodies in water, in which certain substances have been infused; and of which there are a great many different kinds. These animalcules are sometimes found in water which we would call pure, did not the microscope discover its minute inhabitants; but not equally in all kinds of water, or even in all parts of the same kind of it. The surfaces of infusions are generally covered with a scum which is easily broken, but acquires thickness by standing. In this scum the greatest number of animalcules are usually found. Sometimes it is necessary to dilute the infusions; but this ought always to be done with water, not only distilled, but viewed through a microscope, lest it should also have animalcules in it, and thus prove a source of deception. It is, however, most proper to observe those minute objects after the water is a little evaporated; the attention being less diverted by a few objects than when they appear in great number. One or two of the animalcules may be separated from the rest by placing a small drop of water on the glass near that of the infusion; join them together by making a small connexion between them with a pin; and as soon as you perceive that an animalcule has entered the microscope, clear drop, cut off the connexion again.
Eels in paste are obtained by boiling a little flour and water into the confluence of bookbinders paste; then exposing it to the air in an open vessel, and beating it frequently together to keep the surface from growing mouldy or hard. In a few days it will be found peopled with myriads of little animals visible to the naked eye, which are the eels in question. They may be preserved for a whole year by keeping the paste moistened with water; and while this is done, the motion of the animals will keep the surface from growing mouldy. Mr Baker directs a drop or two of vinegar to be put into the paste now and then. When they are applied to the microscope, the paste must be diluted in a piece of water for them to swim in.
Numberless animalcules are observed by the microscope in infusions of pepper. To make an infusion for this purpose, bruise as much common black pepper as will cover the bottom of an open jar, and lay it therein about half an inch thick: pour as much soft water into the vessel as will rise about an inch above the pepper. Shake the whole well together: after which they must not be stirred, but be left exposed to the air for a few days; in which time a thin pellicle will be formed on the surface, in which innumerable animals are to be observed by the microscope.
The microscopic animals are so different from those of the larger kinds, that scarce any sort of analogy seems to exist between them; and one would almost be tempted to think that they lived in consequence of laws directly opposite to those which preserve ourselves and other visible animals in existence. They have been systematically arranged by O. F. Muller; though it is by no means probable that all the different classes have yet been discovered. Such as have been observed, however, are by this author divided in the following manner:
I. Such as have no external organs.
1. Monas: Punctiforme. A mere point. 2. Proteus: Mutabilis. Mutable. 3. Volvox: Sphaericum. Spherical. 4. Enchelis: Cylindracea. Cylindrical. 5. Vibrio: Elongatum. Long.
* Membranaceous.
6. Cyclidium: Ovalis. Oval. 7. Paramecium: Oblongum. Oblong. 8. Kolpoda: Sinuatum. Sinuous. 9. Gonium: Angulatum. With angles. 10. Bursaria. Hollow like a purse.
II. Those that have external organs.
* Naked, or not enclosed in a shell.
1. Cercaria: Caudatum. With a tail. 2. Trichoda: Crinitum. Hairy. 3. Kerona: Corniculatum. With horns. 4. Himantopus: Cirratus. Ciliated. 5. Leucophra: Ciliatum undique. Every part ciliated. 6. Vorticella: Ciliatum apice. The apex ciliated.
* Covered with a shell.
7. Brachionus: Ciliatum apice. The apex ciliated. In the treatise on Helminthology under the fifth order of the class vermes, viz. Infusoria, the genera here enumerated have already been noticed according to an arrangement somewhat different, and a few of the species have been described. For the sake of those who wish to prosecute microscopical inquiries we shall introduce descriptions of a few more, and particularly those whose habitats are known.
I. Monas.
This is by our author defined to be "an invisible (to the naked eye), pellucid, simple, punctiform worm;" but of which, small as it is, there are several species.
The monas terma or gelatinosa, is a small jelly-like point, which can be but imperfectly seen by the single microscope, and not at all by the compound one. In a full light they totally disappear, by reason of their transparency. Some infusions are so full of them that scarce the least empty space can be perceived; the water itself appearing composed of innumerable globular points, in which a motion may be perceived somewhat similar to that which is observed when the sun's rays shine on the water; the whole multitude of animals appearing in commotion like a hive of bees. This animal is very common in ditch-water, and in almost all infusions either of animal or vegetable substances.
Monas atomus or albidus; white monas with a variable point. This appears like a white point, which through a high magnifier appears somewhat egg-shaped. The smaller end is generally marked with a black point, the situation of which is variable; sometimes it appears on the large end, and sometimes there are two black spots in the middle. This species was found in sea water, which had been kept through the whole winter, but was not very fetid. No other kind of animalcule was found in it.
II. The Proteus.
An invisible, very simple, pellucid worm, of a variable form.
The tenax, running out into a fine point. This is a pellucid gelatinous body, stored with black molecules, and likewise changing its figure, but in a more regular order than the former. It first extends itself in a straight line, the lower part terminating in a bright acute point. It appears to have no intestines; and when the globules are all collected in the upper part, it next draws the pointed end up toward the middle of the body, which assumes a round form. It goes through a number of different shapes, part of which are described under the article Animalcule. It is found in some kinds of river water, and appears confined almost entirely to one place, only bending sideways.
III. Volvox.
An invisible, very simple, pellucid, spherical worm.
The punctum; of a black colour, with a lucid point. This is a small globule, with one hemisphere opaque and black, the other having a crystalline appearance; and a vehement motion is observed in the black part.
IV. Enchelis.
A simple, invisible, cylindric worm.
The viridis, or green enchelis, has an obtuse tail, the fore part terminating in an acute truncated angle; the intestines are obscure and indistinct. It continually varies its motion, turning from right to left.
The punctifera, having the fore part obtuse, the hinder part pointed. It is opaque, and of a green colour, with a small pellucid spot in the fore part, in which two black points may be seen; and a kind of double band crosses the middle of the body. The hinder part is pellucid and pointed, with an incision supposed to be the mouth, at the apex of the fore part. It is found in marshes.
The pupula, with the fore part papillary, is found in dunghill water in November and December: it has a rotatory motion on a longitudinal axis, and moves in an oblique direction through the water. Both ends are obtuse; and the hinder part is marked with a transparent circle, or circular aperture.
V. Vibrio.
A very simple, invisible, round, and rather long worm.
The lineola is found in most vegetable infusions in such numbers, that it seems to fill up almost the whole of their substance. It is so small, that with the best magnifiers we can discern little more than an obscure tremulous motion among them. It is more slender than the monas terma.
The serpens, with obtuse windings or flexures, is found in river water, but seldom. It is slender and gelatinous, resembling a serpentine line, with an intestine down the middle.
The spirillum is exceedingly minute, and twisted in a spiral form, which seems to be its natural shape as it never untwists itself, but moves forward in a straight line, vibrating the hind and fore parts. It was found in 1782 in an infusion of the fenches arvensis.
The vermicularis has a milky appearance, with an obtuse apex, and a languid undulatory motion, like that of the common worm. It is found in marshy water in November, Microscope. November, but seldom. It is thought to be the animal mentioned by Leeuwenhoek as found in the dung of the frog and spawn of the male libellula.
The sagitta, with a fetaeous tail, has a long and flexible body; broadest about the middle, and filled there also with gray molecules; the fore part being drawn out into a thin and transparent neck, and the upper end thick and black. It is found in salt water, and seems to move by contracting and extending its neck.
VI. Cyclidium.
A simple, invisible, flat, pellucid, orbicular or oval worm.
The bulla, or orbicular bright cyclidium. This is found occasionally in an infusion of hay. It is very pellucid and white, but the edges somewhat darker than the rest. It moves slowly, and in a semicircular direction.
The millium is very pellucid, and splendid like crystal; and of an elliptical figure, with a line through the whole length of it. The motion is swift, interrupted, and fluttering.
VII. Paramecium.
An invisible, membranaceous, flat, and pellucid worm.
The chrysalis is found in salt water, and differs very little from the former, only the ends are more obtuse, and the margins are filled with black globules.
The verfum is found in ditches, and has an oblong, green, and gelatinous body, filled with molecules; the lower part thicker than the other; and both ends obtuse. It propagates by division.
VIII. Kolpoda.
An invisible, pellucid, flat, and crooked worm.
The lamella is very seldom met with. It resembles a long, narrow, and pellucid membrane, with the hind part obtuse, narrower, and curved towards the top. It has a vacillatory and very singular motion; going upon the sharp edge, not on the flat side as is usual with microscopic animals.
The gallinula is found in fetid salt water; and has the apex somewhat bent, the belly oval, convex, and striated.
The rostrum is found, though seldom, in water where the lemma grows; and has a slow and horizontal motion. The fore part is bent into a kind of hook; the hind part obtuse, and quite filled with black molecules.
The triguetra was found in salt water, and appears to consist of two membranes; the upper side flattened, the lower convex, with the apex bent into a kind of shoulder.
The assimilis is found on the sea-coast, and has an elliptic mass in the middle, but is not folded like the former. The margin of the fore part is notched from the top to the middle; the lower part swells out, and contracts again into a small point.
The cucullulus is found in an infusion of the sonchus arvensis. It is very pellucid and crystalline, with several globules, and has an oblique incision a little below Microcope the apex.
The ren, or crassa, is found in an infusion of hay, commonly about 13 hours after the infusion is made, and has a quick and vacillatory motion. Its body is yellow, thick, and somewhat opaque; curved a little in the middle, so that it resembles a kidney; and full of molecules. When the water in which it swims is about to fail, it takes an oval form, is compressed, and at last bursts.
IX. Gonium.
An invisible, simple, smooth, and angular worm.
The pulvinatum is found in dunghills; and appears like a little quadrangular membrane, plain on both sides; but with a large magnifier it appears like a bolster formed of three or four cylindric pillows sunk here and there.
The corrugatum is found in various kinds of infusions; and is somewhat of a square shape, very small, and in some positions appears as streaked.
The truncatum is found chiefly in pure water, and then but seldom. It has a languid motion, and is much larger than the foregoing. The fore part is a straight line, with which the sides form obtuse angles, the end of the sides being united by a curved line. The internal molecules are of a dark green, and there are two little bright vehicles in the middle.
X. Bursaria.
A very simple, hollow, membranaceous worm.
The truncatella is visible to the naked eye; white, oval, and truncated at the top, where there is a large aperture descending towards the base. Most of them have four or five yellow eggs, at the bottom. They move from left to right, and from right to left; ascending to the surface in a straight line, and sometimes rolling about while they descend.
The bullina is pellucid and crystalline, having splendid globules of different sizes swimming about with it. The under side is convex, the upper hollow, with the fore part forming a kind of lip.
The hirundinella has two small projecting wings, which give it somewhat of the appearance of a bird; and it moves something like a swallow. It is invisible to the naked eye; but by the microscope appears a pellucid hollow membrane.
The dupletta was found among duckweed, and appears like a crystalline membrane folded, up, without any visible intestines except a small congeries of points, under one of the folds.
XI. Cercaria.
An invisible transparent worm with a tail.
The gyrinus greatly resembles the spermatic animalcules. It has a white gelatinous body; the fore part somewhat globular; the hind part round, long, and pointed. Sometimes it appears a little compressed on each side. When swimming it keeps its tail in continual vibration like a tadpole.
The gibba is found in the infusions of hay and other vegetables; The *inquieta* is found in salt water, and is remarkable for changing the shape of its body: sometimes it appears spherical, sometimes like a long cylinder, and sometimes oval. It is white and gelatinous, the tail filiform and flexible, the upper part vibrating violently. A pellucid globule may be observed at the base, and two very small black points near the top.
The *turbo*, with a tail like a brittle, is found among duckweed. It is of a talcy appearance, partly oval and partly spherical; and seems to be composed of two globular bodies, the lowermost of which is the smallest, and it has two little black points like eyes on the upper part. The tail is sometimes straight, sometimes turned back on the body.
The *poduria* is found in November and December, in marshy places covered with lemma. It is pellucid; and seems to consist of a head, trunk, and tail: the head resembles that of a herring; the trunk is ventricose and full of intestines, of a spiral form and black colour. The tail most commonly appears to be divided into two bristles. The intestines are in a continual motion when the body moves, and by reason of their various shades make it appear very rough. There are likewise some hairs to be perceived. It turns round as upon an axis when it moves.
The *viridis* is found in the spring in ditches of standing water; and in some of its states has a considerable resemblance to the last, but has a much greater power of changing its shape. It is naturally cylindrical, the lower end sharp, and divided into two parts; but sometimes contracts the head and tail so as to assume a spherical figure.
The *selviera* is found in salt water, but seldom. It is small, the body rather opaque, and of a round figure. The upper part is bright, and smaller than the rest: the trunk is more opaque; the tail sharp, and near it a little row of short hairs. It has a slow rotary motion.
The *hirta* was likewise found in salt water. It is opaque and cylindrical; and when in motion, the body appears to be surrounded with rows of small hairs separated from each other.
The *pleuronectes* is found in water which has been kept for several months. It is membranaceous, roundish, and white, with two blackish points in the fore part, the hinder part being furnished with a slender sharp tail. It has orbicular intestines of different sizes in the middle; the larger of them bright. The motion is vacillatory; and in swimming it keeps one edge of the lateral membrane upwards, the other folded down.
The *trinos* is flat, pellucid, triangular, having each angle of the base or fore part bent down into two linear arms, the apex of the triangle prolonged into a tail. It is found in salt water.
**XII. Leucophra.**
An invisible, pellucid, and ciliated worm.
The *mamilla* is of a dark colour, and filled with globular molecules; short hairs are curved inwards; and it occasionally projects and draws in a little white protuberance. It is pretty common in marshy water.
The *viridescens* is a large, pear-shaped, greenish-coloured animalcule, filled with opaque molecules, and covered with short hairs; generally moving in a straight line. It is found in salt water.
The *burfata* is found in salt water, and is similar in many respects to the former. It is of a long oval shape, bulging in the middle, and filled with green molecules, everywhere ciliated except at the apex, which is truncated and shaped somewhat like a purse; the hairs are sometimes collected into little fascicles.
The *peshuma* is globular, and covered as it were with a pellucid net; it is found in fetid salt water.
The *figurata* is common in salt water in the months of November and December. It is oblong and subdepressed, with a black margin filled with little molecules, but more particularly distinguished by a curved line in the middle somewhat in the shape of the letter S; one end of which is sometimes bent into the form of a small spiral.
**XIII. Trichoda.**
An invisible, pellucid, hairy worm.
The *gyrus* is one of the smallest of this genus, and is found in salt water. It is smooth and free from hairs, except at the fore part, where there are a few.
The *nigra* was found in salt water, and has an opaque body; but when at rest one side appears pellucid. When in violent motion, it seems entirely black.
The *pubes* is found in water where duckweed grows, chiefly in the month of December. It has a bunch above the hind part marked with black spots, depressed towards the top, a little folded, and somewhat convex on the under part. The apex is furnished with hairs, but they are seldom visible till the creature is in the agonies of death, when it extends and moves them vehemently, and attempting as it were to draw in the very last drop of water.
The *patens* is found in salt water; and is of a long cylindrical shape, filled with molecules, the fore part bright and clear, with a long opening near the top which tapers to a point, and is bejewelled with hairs.
The *florata* is found in the month of December in river water. It is a beautiful animalcule, of a fox colour. It is of an oblong shape, the lower end somewhat larger than the other. It has a set of streaks running from one end to the other, and at the abdomen a double row of little eggs lying in a transverse direction.
The *neula* is found in the infusion of hay and other vegetables. It is five times longer than broad, round, flexuous, of an equal size, the greater part filled with obscure molecules; the fore part rather empty, with an alimentary canal and lucid globules near the middle. The margin of the fore part is covered with short hairs.
The *linter* is found in an infusion of old grass. It is egg-shaped, oblong, with both extremities raised so that the bottom becomes convex, and the upper part depressed like a boat: it is of different shapes at different ages, and sometimes has a rotatory motion.
The *pavillus* is found in salt water; and is long, full of gray molecules; the fore part truncated and hairy, and rather smaller than the other.
The *vermicularis* is found in river water; and is pellucid. The melitea is found in salt water, but very rarely. It is oblong, ciliated, with a globular apex, a dilatable neck, and a kind of peristaltic motion perceivable within it.
The perillum is frequently found in marshes. It is cylindrical, pellucid, muscular, and capable of being folded up. It appears double; the interior part full of molecules, with an orbicular molecular appendage, which it can open and shut, and which forms the mouth. The external part is membranaceous, pellucid, dilated, and marked with transverse streaks; and it can protrude or draw in the orbicular membrane at pleasure. Some have four articulations in the tail, others five; and it has two pairs of brittle, one placed at the second joint, the other at the last.
The delphis is found in river water. It is smooth, pellucid, having the fore part dilated into a fencircle, gradually decreasing in breadth towards the tail. The front is hairy, the hairs standing as rays from the fencircular edge: one of the edges is sometimes contracted.
The delphitus is found in hay that has been infused for some months. It is pellucid, smooth, and egg-shaped; the hinder part terminating in a tail about half the length of the body, dilated at the upper end, truncated, and always bent upwards. It moves sometimes on its belly and sometimes on its side.
The rostrata is found in water where duckweed has been kept. It is depressed, capable of changing its shape, yellow, with long ciliated hairs; it has four feet tapering to a point, one of them longer than the rest. Both feet and hairs are within the margin. The shape of the body is generally triangular; the apex formed into an obtuse beak, which the creature sometimes draws in so that it appears quite round.
The charon was found in salt water. It is oval, and resembles a boat as well in its motion as shape; the upper part is hollowed, the under part furrowed and convex; the stern round, with several hairs proceeding from it.
XIV. Kerona.
An invisible worm with horns.
The rafellum is found in river water. It has three rows of horns on the back, which occupy almost the whole of it.
The cypris is found in water covered with lichen. It is somewhat of a pear shape, compressed, with a broad and blunt fore part; the front furnished with hairs, or little vibrating points inserted under the edge, shorter in the hind part, partly extended straight, and partly bent down, having a retrograde motion.
The calvium is found in the infusion of vegetables. The body is broad and flat, both sides obtuse, filled with black molecules, and there is a black spot near the hinder part, where there are likewise a few short brittle.
The pululata is found in salt water. It is oval, convex; one edge of the hinder part finned, both ends set with hairs, and some horns on the fore part.
XV. Himantopus.
A pellucid, invisible, and cirrated worm.
The acarus is lively, conical, ventricose, full of black molecules, with a bright and transparent fore part. The lower part of the apex has rows of long hairs on the under part set like rays. Four locks of long crooked hair or feet proceed from the belly, and it is continually moving these and other hairs in various directions.
The ludio is a lively diverting animalcule, smooth, pellucid, full of small points, the fore part clubbed and a little bent, the hinder part narrow; the base obliquely truncated, and terminating in a tail stretched out tranversely. The top of the head and middle of the back are furnished with long and vibrating hairs; three moveable and flexible curls hang down from the side of the head at a distance from each other. When the creature is at rest, its tail is curled; but when in motion, it is drawn tight and extended upwards.
The fannio is found, though seldom, in water where the lemma grows. The cilia are longer than the hairs, and are continually vibrating: it has two moveable curls hanging on the side of the head.
The charon is found in sea water, but rarely. It is oval, pellucid, and membranous, with longitudinal furrows and several bent diverging rows of hair below the middle, but none on the hinder part.
XVI. Vorticella.
A naked worm with rotatory cilia, capable of contracting and extending itself.
The lunfera, is found in salt water; has the fore part obtuse, the base broad, and hollowed away like a crescent, with a short protuberance in the middle of the concave part: the fore part is ciliated.
The burfata is found in salt water, and is ventricose, crammed with molecules; the fore part truncated, and both sides of it pellucid: there is a prominent papilla in the middle, which when the animalcule is at rest appears notched, the edge of the aperture being ciliated; the hairs are capable of moving in various directions.
The futarium is found in October, with the lesser lemma, and is one of the most singular of the microscopic animalcules. When viewed sidewise, it is sometimes nearly cylindrical, only tapering a little towards the hinder part, and having a broad pellucid edge. Viewed from the top, it has sometimes a broad face or disk, furnished with radiating hairs, the under part contracted into a globular shape, of a dark green colour, and filled with small grains.
The multiformis is found in salt water, and very much resembles the former.
The nigra is found in August in meadows covered with water. It may be seen with the naked eye, appearing like a black point swimming on the surface. Through the microscope it appears as a small conical body, obtuse and ventricose at one end, and acute at the other. When the extremities are extended, two small white hooks become visible, by the assistance of which Microscope, which it moves in the water, and it probably has a rotatory organ: it moves continually in a vacillating manner on the top of the water.
The ocreata is met with in rivers, though very seldom, and in shape somewhat resembles the lower part of a boot. The apex of the upper part is truncated and ciliated, the heel pointed, and the foot round.
The valga is as broad as long, and the apex truncated and ciliated; both angles of the base projecting outwards, one somewhat like a wart, the other like a finger. It is found in marshy waters.
The papillaris is likewise found in marshes where the conserva nitida grows. It is ventricose; the fore part truncated, with a papillary tail, and a beautiful papillary excretion on the side.
The crategaria is found in the month of April, both in the mud and on the tail of the monoculus quadricornis. They are generally heaped together in a spherical form, and united to one common stalk. They are likewise often to be found without a pedicle, the body rather contracted, the aperture circular, and surrounded with a marked margin. It has two small arms; and with a powerful magnifier a violent rotatory motion may be observed. Sometimes an individual will separate from the community, and move in a kind of spiral line for a little time, and then go back to the rest.
The rotatorius is the wheel animal described by Mr Baker; and of which an account is given under the article Animalcule.
The furcata is commonly found in water, and has a cylindric body with a rotatory organ, consisting of a row of hairs at the apex: the tail is divided into two parts, turning a little inwards. When at rest it joins the segments of the tail, but opens them when in motion.
The citrina is found in stagnant water; the head full of molecules, round, everywhere of an equal size, and very transparent. Both sides of the orifice are ciliated, and each has a rotatory motion appearing sometimes without and sometimes within the edge of the mouth.
The convallaria is the same with the bell-animal mentioned by Mr Baker. See the article Animalcule.
The acina inhabits that whitish substance which often entirely covers plants, wood, shells, &c. When this substance is examined by a microscope, it appears to be wholly composed of living animals of the polype kind. See Polype.
The pyraria. The anaflatica. See the article Polype. The digitalis.
XVII. Brachionus.
A contractile worm, covered with a shell, and furnished with rotatory cilia.
The patella is found in marshy water in the winter-time. It is univalve, the shell oval, plain, crystal-line, with the anterior part terminating in two acute points on both sides, though the intervening space is commonly filled up with the head of the animal. By these points it fastens itself, and whirls about the body erect. The rotatory cilia are perceived with great difficulty.
To what has been already said on this subject, under Microscope, the article Animalcule, we shall here add the following observations from Mr Adams.—"How many kinds of these invisibles there may be (says he), is yet unknown; as they are discerned of all sizes, from those which are barely invisible to the naked eye, to such as resist the force of the microscope as the fixed stars do that of the telescope, and with the greatest powers hitherto invented appear only as so many moving points. The smallest living creatures our instruments can show, are those which inhabit the waters; for though animalcula equally minute may fly in the air, or creep upon the earth, it is scarcely possible to get a view of them; but as water is transparent, by confining the creatures within it we can easily observe them by applying a drop of it to the glasses.
"Animalcules in general are observed to move in all directions with equal ease and rapidity, sometimes obliquely, sometimes straight forward; sometimes moving in a circular direction, or rolling upon one another, running backwards and forwards through the whole extent of the drop, as if diverting themselves; at other times greedily attacking the little parcels of matter they meet with. Notwithstanding their extreme minuteness, they know how to avoid obstacles, or to prevent any interference with one another in their motions: sometimes they will suddenly change the direction in which they move, and take an opposite one; and, by inclining the glass on which the drop of water is, as it can be made to move in any direction, so the animalcules appear to move as easily against the stream as with it. When the water begins to evaporate, they flock towards the place where the fluid is, and show a great anxiety and uncommon agitation of the organs with which they draw in the water. These motions grow languid as the water fails, and at last cease altogether, without a possibility of renewal if they be left dry for a short time. They sustain a great degree of cold as well as insects, and will perish in much the same degree of heat that destroys insects. Some animalcules are produced in water at the freezing point, and some insects live in snow. —By mixing the least drop of urine with the water in which they swim, they instantly fall into convulsions and die.
"The same rule seems to hold good in those minute creatures, which is observable in the larger animals, viz. that the larger kinds are less numerous than such as are smaller, while the smallest of all are found in such multitudes, that there seem to be myriads for one of the others. They increase in size, like other animals, from their birth until they have attained their full growth; and when deprived of proper nourishment, they in like manner grow thin and perish."
The modes of propagation among these animalcules are various, and the observation of them is extremely curious. Some multiply by a transverse division, as is observed under the article Animalcule: and it is remarkable, that though in general they avoid one another, it is not uncommon, when one is nearly divided, to see another push itself upon the small neck which joins the two bodies in order to accelerate the separation.—Others, when about to multiply, fix themselves to the bottom of the water; then becoming first oblong, and afterwards round, turn rapidly as on a centre, but perpetually varying the direction of their rotatory motion. Microscope. motion. In a little time, two lines forming a cross are perceived; after which the spherule divides into four, which grow, and are again divided as before. A third kind multiply by a longitudinal division, which in some begins in the fore part, in others in the hind part; and from others a small fragment detaches itself, which in a short time assumes the shape of the parent animalcule. Lastly, others propagate in the same manner as the more perfect animals.
In our observations under the article Animalcula, we suggested some doubts whether all those minute bodies which go under the name of animalcules really do enjoy animal life; or whether they are not in many cases to be accounted only inanimate and exceedingly minute points of matter actuated by the internal motion of the fluid. This has also been the opinion of others: but to all hypotheses of this kind Mr Adams makes the following reply: "From what has been said, it clearly appears, that their motions are not purely mechanical, but are produced by an internal spontaneous principle; and that they must therefore be placed among the class of living animals, for they possess the strongest marks and the most decided characters of animation; and, consequently, that there is no foundation for the supposition of a chaotic and neutral kingdom, which can only have derived its origin from a very transient and superficial view of these animalcules.—It may also be further observed, that as we see that the motions of the limbs, &c., of the larger animals, are produced by the mechanical construction of the body, and the action of the soul thereon, and are forced by the ocular demonstration which arises from anatomical dissection to acknowledge this mechanism which is adapted to produce the various motions necessary to the animal; and as, when we have recourse to the microscope, we find those pieces which had appeared to the naked eye as the primary mechanical causes of particular motions, to consist themselves of lesser parts, which are the causes of motion, extension, &c., in the larger; when the structure therefore can be traced no farther by the eye, or by the glasses, we have no right to conclude that the parts which are invisible are not equally the subject of mechanism: for this would be only to affirm, in other words, that a thing may exist because we see and feel it, and have no existence when it is not the object of our senses.—The same train of reasoning may be applied to microscopic insects and animalcules: we see them move; but because the muscles and members which occasion these motions are invisible, shall we infer that they have not muscles, with organs appropriated to the motion of the whole and its parts? To say that they exist not because we cannot perceive them, would not be a rational conclusion. Our senses are indeed given us that we may comprehend some effects; but then we have also a mind, with reason, bestowed upon us, that, from the things which we do perceive with our senses, we may deduce the nature of those causes and effects which are imperceptible to the corporeal eye."
Leaving these speculations, however, we shall now proceed to give a particular
Explanation of the figures of the various animals, with their parts, ova, &c., represented in the plates.
Plate CCXLII. Fig. 32, 33, represent the eggs of the phalæna fig. 32, 33, neulria, as they are taken from the tree to which they adhere, and magnified by the microscope. The microscope, strong ground-work visible in many places shows the gum by which they are fastened together; and this connexion is strengthened by a very tenacious substance interposed between the eggs, and filling up the vacant spaces. Fig. 34, shows a vertical section of the eggs, exhibiting their oval shape.—Fig. 35, is an horizontal section through the middle Fig. 35 of the egg. These eggs make a beautiful appearance through the microscope. The small figures a, b, c, represent the objects in their natural state, without being magnified.
Fig. 36, shows the larva of the musca chameleon, an aquatic insect. When viewed by the naked eye, it appears (as here represented) to be composed of twelve annular divisions, separating it into an head, thorax, and abdomen; but it is not easy to distinguish the two last parts from each other, as the intestines lie equally both in the thorax and abdomen. The tail is furnished with a fine crown or circle of hair b, disposed in the form of a ring, and by this means it is supported on the surface of the water, the head and body hanging down towards the bottom, in which posture it will sometimes remain for a considerable time without any motion.—When it has a mind to sink to the bottom, it closes the hairs of the ring, as in fig. 37. Thus an hole Fig. 37 low space is formed, including a small bubble of air; by enlarging or diminishing which, it can rise or sink in the water at pleasure. When the bubble escapes, the insect can replace it from the pulmonary tubes, and sometimes considerable quantities of air may be seen to escape from the tail of the worm into the common atmosphere; which operation may easily be observed when the worm is placed in a glass of water, and affords an entertaining spectacle. The front of this insect is divided into three parts, of which that in the middle is immovable; the other two, which grow from the sides of the middle one, are moveable, and vibrate like the tongues of lizards or serpents. In these lateral parts lies most of the creature's strength; for it walks upon them when out of the water, appearing to walk on its mouth, and to use it as the parrot does its beak to assist it in climbing.
The larva is shown, fig. 38, as it appears through a microscope. It grows narrower towards the head, is largest about that part, which we may call the thorax, converges all along the abdomen, and terminates at length in a sharp tail surrounded with hairs, as has already been mentioned. The twelve annular divisions are now extremely visible, and are marked by numbers in the plate. The skin appears somewhat hard, and resembling flaggeen, being thick set with grains pretty equally distributed. It has nine holes, or spiracula, probably for the purpose of breathing, on each side; but it has none of these on the tail division a, nor any easily visible on the third from the head. In the latter, indeed, it has some very small holes concealed under the skin, near the place where the embryonic wings of the future fly are hid. "It is remarkable (says Mr Adams) that caterpillars, in general, have two rings without these spiracula, perhaps because they change into flies with four wings, whereas this worm produces a fly with only two." The skin of the larva is adorned with oblong black furrows, spots of a light colour, and orbicular rings, from which there generally springs Microscope. a hair; but only those hairs which grow on the insect's sides are represented in the figure. There are also some larger hairs here and there, as at c c. The difference of colour, however, in this worm arises only from the quantity of grains in the same space; for where they are in very great numbers, the furrows are darker, and paler where they are less plentiful.
The head a is divided into three parts, and covered with a skin which has hardly any discernible grains.—The eyes are rather protuberant, and lie near the snout; on which last are two small horns at i i. It is crooked and ends in a sharp point as at f. The legs are placed near the snout between the sinuses in which the eyes are fixed. Each of these legs consists of three joints, the outermost of which is covered with stiff hairs like bristles g g. From the next joint there springs a horny bone h h, used by the insect as a kind of thumb: the joint is also composed of a black substance of an intermediate hardness between bone and horn; and the third joint is of the same nature. In order to distinguish these parts, those that form the upper sides of the mouth and eyes must be separated by means of a small knife; after which, by the assistance of the microscope, we may perceive that the leg is articulated by some particular ligaments, with the portion of the insect's mouth which answers to the lower jaw in the human frame. We may then also discern the muscles which serve to move the legs, and draw them up into a cavity that lies between the snout and those parts of the mouth which are near the horns i i. The insect walks upon these legs, not only in the water, but on the land also. It likewise makes use of them in swimming, keeping its tail on the surface contiguous to the air, and hanging downward with the rest of the body in the water. In this situation, the only perceptible motion it has is in its legs, which it moves in a most elegant manner, from whence it is reasonable to conclude, that the most of this creature's strength lies in its legs, as we have already observed.
The snout of this larva is black and hard; the back part quite solid, and somewhat of a globular form; the front f sharp and hollow. Three membranaceous divisions may be perceived on the back part; by means of which, and the muscles contained in the snout, the creature can contract or expand it at pleasure.
The extremity of the tail is surrounded with thirty hairs, and the sides adorned with others that are smaller; and here and there the large hairs branch out into smaller ones, which may be reckoned single hairs. All these have their roots in the outer skin, which in this place is covered with rough grains, as may be observed by cutting it off and holding it against the light upon a slip of glass. Thus also we find, that at the extremities of the hairs there are grains like those on the skin; and in the middle of the tail there is a small opening, within which are minute holes, by which the insect takes in and lets out the air it breathes. These hairs, however, are seldom disposed in such a regular order as is represented in fig. 38, unless when the insect floats with the body in the water, and the tail with its hairs a little lower than the surface, in which case they are disposed exactly in the order delineated in the plate. The least motion of the tail downward produces a concavity in the water; and it then assumes the figure of a wine glass, wide at the top and narrow at the bottom. The tail answers the double purpose of swimming and breathing, and through it the insect receives what is the principle of life and motion to all animals. By means of these hairs also it can stop its motion when swimming, and remain suspended quietly without motion for any length of time. Its motions in swimming are very beautiful, especially when it advances with its whole body floating on the surface of the water, after filling itself with air by the tail.—To set out, it first bends the body to the right or left, and then contracts it in the form of the letter S, and again stretches it out in a straight line: by thus contracting and then extending the body alternately, it moves on the surface of the water. It is very quiet, and is not disturbed by handling.
These creatures are commonly found in shallow standing waters in the beginning of June: but some years much more plentifully than others. They crawl on the grass and other plants which grow in such waters, and are often met with in ditches floating on the surface of the water by means of their tails, the head and thorax at the same time hanging down; and in this posture they turn over the clay and dirt with their snout and feet in search of food, which is commonly a viscous matter met with in small ponds and ditches. It is very harmless, though its appearance would seem to indicate the contrary. It is most easily killed for dissection by spirit of turpentine.
Fig. 39 shows in its natural size a beautiful insect described by Linnaeus under the name of Leucoptera doris, and which appears to be a kind of intermediate genus between a fly and a wasp. The antennae are black and cylindrical, increasing in thickness towards the extremity; the joint nearest the head is yellow; the head and thorax are black, encompassed with a yellow line, and furnished with a cross line of the same colour near the head. The scutellum is yellow, the abdomen black, with two yellow bands, and a deep spot of the same colour on each side between the bands. A deep polished groove extends down the back from the thorax to the anus, into which the sting turns and is deposited, leaving the anus very circular; a yellow line runs on each side of the sting.—The anus and whole body, when viewed with a small magnifier, appear punctuated; but when these points are seen through a large magnifier, they appear hexagonal. Fig. 40 shows the insect very much magnified. Fig. 41 gives a side view of it magnified in a smaller degree.
Fig. 42 shows an insect discovered by Mr John Adams of Edmonton, as he happened to be at an inn. It was first seen by some labouring people who were there at the time, by whom it was conjectured to be a louse with unusually long horns, a mite, &c. Mr Adams hearing the debate, procured the insect; and having viewed it through a microscope, it presented the appearance exhibited in fig. 42. The insect seems to be quite distinct from the phalangium caucrodes of Linnæus. The latter has been described by several authors, but none of their descriptions agree with this. The abdomen of this insect is more extended, the claws larger, and much more obtuse; the body of the other being nearly orbicular, the claws slender, and almost terminating in a point, more transparent, and of a paler colour. Mr Mariham has one Microscope in his possession not to be distinguished from that represented in fig. 42, excepting only that it wants the break or dent in the claws, which is so conspicuous in this. He found that insect firmly fixed by its claws to the thigh of a large fly, which he caught on a flower in Edex in the first week of August, and from which he could not disengage it without great difficulty, and tearing off the leg of the fly. This was done upon a piece of writing paper; and he was surprised to see the little creature spring forward a quarter of an inch, and again seize the thigh with its claws, so that he had great difficulty in disengaging it. The natural size of this creature, which Mr Adams calls the lobster-insect, is exhibited at a.
Fig. 43 shows the insect named by M. de Geer Physapul, on account of the bladders at its feet, (Thrips physapul, Lin.). This insect is to be found in glee plenty upon the flowers of dandelion, &c., in the spring and summer. It has four wings, two upper and two under ones (represented fig. 44,) but the two undermost are not to be perceived without great difficulty. They are very long; and fixed to the upper part of the breast, lying horizontally. Both of them are rather pointed towards the edges, and have a strong nerve running round them, which is set with a hair fringe tufted at the extremity. The colour of these wings is whitish: the body of the insect is black; the head small, with two large reticulated eyes. The antennae are of an equal size throughout, and divided into six oval pieces, which are articulated together.—The extremities of the feet are furnished with a membranaceous and flexible bladder, which it can throw out or draw in at pleasure. It presses this bladder against the substances on which it walks, and thus seems to fix itself to them; the bladder sometimes appears concave towards the bottom, the concavity diminishing as it is less pressed. The insect is represented of its natural size at b.
Fig. 45 represents the cimex striatus of Linnaeus, remarkable for very bright and elegantly disposed colours, though few in number. The head, proboecis, and thorax, are black; the thorax ornamented with yellow spots; the middle one large, and occupying almost one-third of the posterior part; the other two are on each side, and triangular. The scutellum has two yellow oblong spots, pointed at each end. The ground of the elytra is a bright yellow; spotted and striped with black. The nerves are yellow; and there is a brilliant triangular spot of orange, which unites the crustaceous and membranaceous parts; the latter are brown, and clouded. It is found in the elm tree in June. It is represented of its natural size at c.
Fig. 46 shows the chrysonela asparagi of Linnaeus, so called from the larva of the insect feeding upon that plant. It is a common insect, and very beautiful. It is of an oblong figure, with black antennae, composed of many joints, nearly oval. The head is a deep and bright blue; the thorax red and cylindrical; the elytra are blue, with a yellow margin, and having three spots of the same colour on each; one at the base, of an oblong form, and two united with the margin: the legs are black; but the under side of the belly is of the same blue colour with the elytra and head. This little animal, when viewed by the naked eye, scarcely appears to deserve any notice; but when examined by Microscope, the microscope, is one of the most pleasing opaque objects we have. It is found in June on the asparagus after it has run to seed; and it is shown of its natural size at d. De Geer says that it is very scarce in Sweden.
Fig. 47 shows an insect of a shape so remarkable, Fig. 47, that naturalists have been at a loss to determine the genus to which it belongs. In the Fauna Suecica, Linnaeus makes it an attelabus; but in the last edition of the Systema Naturae, it is ranged as a meloe, under the title of the Meloe monoceros; though of this also there seems to be some doubt. The true figure of it can only be discovered by a very good microscope. The head is black, and appears to be hid or buried under the thorax, which projects forward like a horn: the antennae are composed of many joints, and are of a dirty yellow colour, as well as the feet: the hinder part of the thorax is reddish, the fore part black.—The elytra are yellow, with a black longitudinal line down the centre; there is a band of the same colour near the apex, and also a black point near the tale, the whole animal being curiously covered with hair. The natural size of it is shown at e. It was found in May. Geoffroy says that it lives upon umbelliferous plants.
Fig. 48—54 exhibit the anatomy of the cossus caterpillar, which lives on the willow. The egg from CCCXLIII, which it proceeds is attached to the trunk of the tree by a kind of viscid juice, which soon becomes so hard that the rain cannot dissolve it. The egg itself is very small and spheroidal, and, when examined by the microscope, appears to have broad waving furrows running through the whole length of it, which are again crossed by close streaks, giving it the appearance of a wicker basket. It is not exactly known what time they are hatched; but as the small caterpillars appear in September, it is probable that the eggs are hatched some time in August. When small, they are generally met with under the bark of the tree to which the eggs were affixed; and an aqueous moisture, oozing from the hole through which they got under the bark, is frequently, though not always, a direction for finding them. These caterpillars change their colour but very little, being nearly the same when young as when old. Like many others, they are capable of spinning as soon as they come from the egg. They also change their skin several times; but as it is almost impossible to rear them under a glass, so it is very difficult to know exactly how often this moulting takes place.—Mr Adams conjectures that it is more frequently than the generality of caterpillars do, some having been observed to change more than nine times.
The cossus generally fasts for some days previous to the moulting; during which time the fleety and other interior parts of the head are detached from the old skull, and retire as it were within the neck. The new coverings soon grow on, but are at first very soft.—When the new skin and the other parts are formed, the old skin is to be opened, and all the members withdrawn from it; an operation naturally difficult, but which must be rendered more so from the soft and weak state of the creature at that time. It is always much larger after each change.
From Mr Lyonet's experiments, it appears, that the Microscope. colus generally passes at least two winters, if not three, before it assumes the pupa state. At the approach of winter, it forms a little case, the inside of which is lined with silk, and the outside covered with wood ground like very fine saw-dust. During the whole season it neither moves nor eats.
This caterpillar, at its first appearance, is not above one-twelfth of an inch long; but at last attains the length of two, and sometimes of three inches. In the month of May it prepares for the pupa state; the first care being to find a hole in the tree sufficient to allow the moth to illude forth; and if this cannot be found, it makes one equal in size to the future pupa. It then begins to form of wood a case or cone; uniting the bits, which are very thin, together by silk, into the form of an ellipsoid, the outside being formed of small bits of wood joined together in all directions; taking care, however, that the pointed end of the case may always be opposite to the mouth of the hole: having finished the outside of the case, it lines the inside with a filken tapestry of a clothe texture in all its parts, except the pointed end, where the texture is looser, in order to facilitate its escape at the proper time. The caterpillar then places itself in such a posture, that the head may always lie towards the opening of the hole in the tree or pointed end of its case. Thus it remains at rest for some time: the colour of the skin first becomes pale, and afterwards brown; the interior parts of the head are detached from the skull; the legs withdraw themselves from the exterior case; the body shortens; the posterior part grows small, while the anterior part swells so much, that the skin at last bursts; and, by a variety of motions, is pushed down to the tail; and thus the pupa is exhibited, in which the parts of the future moth may be easily traced.—The covering of the pupa, though at first soft, humid, and white, soon dries and hardens, and becomes of a dark purple colour; the posterior part is moveable; but not the fore part, which contains the rudiments of the head, legs, and wings. The fore-part of the pupa is furnished with two horns, one above and the other under the eyes. It has also several rows of points on its back. It remains for some weeks in the case; after which the moth begins to agitate itself, and the points are then of essential service, by acting as a fulcrum, upon which it may rest in its endeavours to proceed forward, and not slip back by its efforts for that purpose.
The moth generally continues its endeavours to open the case for a quarter of an hour; after which, by redoubled efforts, it enlarges the hole, and presses forward until it arrives at the edge, where it makes a full stop, left by advancing further it should fall to the ground. After having in this manner repose itself for some time, it begins to disengage itself entirely; and having rested for some hours with its head upwards, it becomes fit for action. Mr Marsham says, that it generally pushes one-third of the case out of the hole before it halts.
The body of the caterpillar is divided into twelve rings, marked 1, 2, 3, &c., as represented in figs. 48, 49, 50, 51, each of which is distinguished from that which precedes, and that which follows, by a kind of neck or hollow; and, by forming boundaries to the rings, we make twelve other divisions, likewise expressed in the figures; but to the first of these the word Microscope ring is affixed, and to the second, division. To facilitate the description of this animal, M. Lyonet supposed a line to pass down through the middle of the back, which he called the superior line, because it marked the most elevated part of the back of the caterpillar; and another, passing from the head down the belly to the tail, he called the inferior line.
All caterpillars have a small organ, resembling an elliptic spot, on the right and left of each ring, excepting the second, third, and last; and by these we are furnished with a further subdivision of this caterpillar, viz. by lines passing through the spiracula, the one on the right side, the other on the left of the caterpillar. These four lines, which divide the caterpillar longitudinally into four equal parts, mark each the place under the skin which is occupied by a considerable viscous. Under the superior line lies the heart, or rather thread of hearts; over the inferior line, the spinal marrow; and the two tracheal arteries follow the course of the lateral lines. At equal distances from the superior and two lateral lines, we may suppose four intermediate lines. The two between the superior and lateral lines are called the intermediate superior; the two others opposite to them, and between the lateral and inferior lines, are called the intermediate inferior.
Fig. 48, 49, show the muscles of the caterpillar, arranged with the most wonderful symmetry and order, especially when taken off by equal strata on both sides, which exhibits an astonishing and exact form and correspondence in them. The figures show the muscles of two different caterpillars opened at the belly, and supposed to be joined together at the superior lines. The muscles of the back are marked by capitals; the gastric muscles by Roman letters; the lateral ones by Greek characters. Those marked $ are called, by M. Lyonet, dividing muscles, on account of their situation.
The caterpillar was prepared for dissection by being emptied, and the muscles, nerves, &c. freed from the fat in the manner formerly directed; after which the following observations were made.
The muscle A in the first ring is double; the anterior one being thick at top, and being apparently divided into different muscles on the upper side, but without any appearance of this kind on the under side. One insertion is at the skin of the neck towards the head; the other is a little above; and that of the second muscle A is a little below the first spiraculum, near which they are fixed to the skin.
The muscle marked a is long and slender, fixed by its anterior extremity under the gastric muscles a and b of the first ring, to the circumflex scale of the base of the lower lip. It communicates with the muscle c of the second ring, after having passed under some of the arteries, and introduced itself below the muscle d.
The muscle b is so tender, that it is scarcely possible to open the belly of the caterpillar without breaking it. It is sometimes double, and sometimes triple.—Anteriorly it is fixed to the posterior edge of the side of the parietal scale, the lower fixture being at the middle of the ring near the inferior line.
There are three muscles marked $; the first affixed Microscope at one extremity near the lower edge of the upper part of the parietal scale; the other end divides itself into three or four tails, fixed to the skin of the caterpillar under the muscle $b$. The anterior part of the second is fixed near the first; the anterior part of the third a little under the first and second, at the skin of the neck under the muscle $A$. These two last palping over the cavity of the first pair of limbs, are fixed by several tails to the edge opposite to this cavity. In this subject there are two muscles marked $a$, but sometimes there is only one anteriorly; they are fixed to the lower edge of the parietal scale, the other ends being inserted in the first fold of the skin of the neck on the belly-side. Fig. 50. best represents the muscles $b$ and $c$; as in that figure they do not appear injured by any unnatural connexion.
In the second and four following rings we discern two large dorsal muscles $A$ and $B$. In the 7th, 9th, and 10th rings are three, $A$, $B$, and $C$; in the 11th are four, $A$, $B$, $C$, and $D$; and in the anterior part of the 12th ring are five, $A$, $B$, $C$, $D$, and $E$. All these ranges of muscles, however, as well as the gastric muscles $a$, $b$, $c$, $d$, appear at first sight only as a single muscle, running nearly the whole length of the caterpillar; but when this is detached from the animal, it is found to consist of so many distinct muscles, each consisting only of the length of one of the rings; their extremities being fixed to the division of each ring, excepting the middle muscle $a$, which, at the 6th, 7th, 8th, and 9th rings, has its insertions rather beyond the division. Each row of muscles appears as one, because they are closely connected at top by some of the fibres which pass from one ring to the other.
The muscles $A$, which are 12 in number, gradually diminish in breadth to the lower part of the last ring; at the 8th and three following divisions they communicate with the muscles $B$, and at the 11th with $D$. In the lower part of the last ring, $A$ is much broader than it was in the preceding ring; one extremity of it is contracted, and communicates with $B$; the lower insertion being at the membrane $I$, which is the exterior skin of the fecal bag. The muscles $A$ and $B$, on the lower part of the last ring, cannot be seen until a large muscle is removed, which on one side is fixed to the subdivision of the ring, and on the other to the fecal bag.
The right muscles $B$, which are also 12 in number, begin at the second ring, and grow larger from thence to the seventh. They are usually narrower from thence to the 12th; the deficiency in width being supplied by the fix muscles $C$, which accompany it from the 7th to the subdivision of the 12th ring. The muscles $B$ and $C$ communicate laterally with the 8th, 11th, and 12th divisions. $C$ is wanting at the subdivision of the 12th; its place being here supplied by $B$, which becomes broader at this part.
The first of the three floating muscles $V$ originates at the first ring, from whence it introduces itself under $N$, where it is fixed, and then subdivides, and hides itself under other parts. The second begins at the second division, being fixed to the anterior extremity $B$ of the second ring; from thence directing itself towards the stomach; and after communicating with the case of the corpus cossum, it divides, and spreads into eight muscles which run along the belly. The third begins at the third division, originating partly at the skin, and partly at the junction of the muscles $B$ of the second and third ring. It directs itself obliquely towards the belly, meeting it near the third spiraculum; and branching from thence, it forms the oblique muscles of some of the viscera.
The thin long muscle $e$, which is at the subdivision of the last ring, and covers the anterior insertion of the muscle ($a$) where the ring terminates, is single. It begins at one extremity of the muscle ($c$); at the fore part of the ring runs along the subdivision round the belly of the caterpillar, and finishes, on the other side, at the extremity of a similar muscle $C$.
Fig. 49. shows the dorsal muscles of the coxos. To view which in an advantageous manner, we must use the following mode of preparation.
1. All the dorsal muscles, 35 in number, must be taken out, as well as the seven lateral ones already described.
2. All the straight muscles of the belly must be taken away, as well as the muscular roots ($c$), and the ends of the gastric muscles ($c$), which are at the third and fourth divisions.
3. At the second division the muscle $e$ must be removed; only the extremities being left to show where it was inserted.
The parts being thus prepared, we begin at the third ring; where there are found four dorsal muscles $C$, $D$, $E$, and $F$. The first one $C$, is inserted at the third division, under the muscles $b$ and $a$, where it communicates by means of some fibres with the muscle $f$ of the second ring; proceeding from thence obliquely towards the intermediate superior line, and is fixed at the fourth division. As soon as $C$ is retrenched, the muscle $D$ is seen; which grows wider from the anterior extremity; it lies in a contrary direction to the muscle $C$, and is inserted into the third and fourth divisions. The muscle $E$ lies in the same direction as the middle $C$, but not so obliquely; the lower insertion is at the fourth division; the other at the third, immediately under $C$. The muscle $F$ is nearly parallel to $D$ which joins it; the first insertion is visible, but the other is hid under the muscles $E$ and $G$ at the fourth division.
In the eight following rings, there are only two dorsal muscles; and of these $D$ is the only one that is completely seen. It is very large, and diminishes gradually in breadth from one ring to the other, till it comes to the last, sending off branches in some places.—$E$ is one of the strait muscles of the back; and is inserted under the dividing muscles $e$, at the divisions of its own ring.
On the anterior part of the 12th ring there are three dorsal muscles, $D$, $E$, and $F$. $D$ is similar to that of the preceding ring, marked also $D$, only that it is no more than half the length; terminating at the subdivision of its own ring. $E$ is of the same length, and differs from the muscle $E$ of the preceding ring only in its direction. $F$ is parallel to $E$, and shorter than it; its anterior end does not reach the twelfth division.
On the posterior part there is only one dorsal muscle, fastened by some short ones to the subdivision of the last ring, traversing the muscles $a$; and being fixed there as if designed to strengthen them, and MIC
Microscope, to vary their direction.—z Is a single muscle, of which the anterior insertion is visible, the other end being fixed to the bottom of the foot of the last leg; its use is to move the foot. The anterior part of the muscle β branches into three or four heads, which cross the superior line obliquely, and are fixed to the skin a little above it. The other end is fastened to the membrane T.
Fig. 50. Fig. 51. show the muscles of the caterpillar when it is opened at the back. The preparation for this view is to disengage the fat and other extraneous matter, as before directed.
The first ring has only two gastric muscles (c) and (d): the former is broad, and has three or four little tails: the first fixture is at the base of the lower lip, from whence it extends obliquely, and is fixed between the inferior and lateral line. The small muscle (d) is fastened on one side to the first spiraculum; on the other, a little lower, to the intermediate inferior and lateral line; and seems to be an antagonist to the muscle P, which opens the spiracula. The posterior fixture of δ is under the muscle C, near the skin of the neck; β is fixed a little on the other side of C, at the middle of the ring.
In the second ring there are three gastric muscles, g, h, and i: g and h are fixed at the folds which terminate the ring; but only the anterior part of i is fixed there. The muscle h is triple, and in one of the divisions separated into two parts; that marked i comes nearer the inferior line, and is fixed a little beyond the middle of the ring, where the corresponding muscle of the opposite side is forked to receive it.
In the third ring, the muscle h, which was triple in the foregoing ring, is only double here, that part which is nearest the inferior line being broadest: it has three tails, of which only two are visible in the figure. It is exactly similar to that of the preceding ring; and is crossed in the same manner by the muscle from the opposite side of the ring.
Throughout the eight following rings, the muscle f which runs through them all is very broad and strong. The anterior part of it is fixed at the intermediate inferior line, on the fold of the first division of the ring; the other part is fixed beyond the lower division; with this difference, that at the 10th and 11th rings it is fixed at the last fold of its ring; whereas, in the others it passes over that ring, and is inserted into the skin of the following one. In all these, the first extremity of the muscle g is fastened to the fold which separates the ring from the preceding one, and is parallel to f, and placed at the side of it. The first five muscles marked g, are forked; that of the fourth ring being more so than the rest, nor does it unite till near its anterior insertion. The longest tail lays hold of the following, and is inserted near the inferior line; the other inserts itself near the same line, at about the middle of its own ring; the two last do not branch out; but terminate at the divisions, without reaching the following ring. The muscle h, placed at the side of f, has nearly the same direction, and finishes at the folds of the ring.
The anterior part of the 12th ring has only one gastric muscle, marked e: it is placed on the intermediate inferior line; and is inserted at the folds of the upper division, and at the subdivision of this ring. The lower part has a larger muscle marked c, with several divisions; one placed under b, with one extremity fixed near the lateral line, at the subdivision of its ring; the other to the fecal bag, a little lower than the muscle b.
In fig. 51. all the gastric muscles described in fig. 50. disappear, as well as those lateral and dorsal ones of which the letters are not to be found in this figure.
In the first ring are the gastric muscles, e, f, g, which are best seen here: the first is narrow and long, passing under and crossing f: one of its insertions is at the lower line, the other at the lateral, between the spiraculum and neck; f is short, broad, and nearly straight, placed along the intermediate line; but between it and the lateral it passes under e, and is fixed to the fold of the skin which goes from the one bag to the other; the lower insertion is near the second division. There are sometimes three muscles of those marked g, and sometimes four: the lower parts of them are fixed about the middle of the ring, and the anterior parts at the fold of the skin near the neck. The muscles i and h are fixed to the same fold; the other end of h being fixed under the muscle π, near the spiraculum. Above the upper end of f, a muscular body, g, may be seen. It is formed by the separation of two floating muscles.
The second ring has six gastric muscles, k, l, m, n, o, p. The first is a large oblique muscle, with three or four divisions placed at the anterior part of the ring: the head is fixed between the inferior line and its intermediate one, at the fold of the second division; from whence it crosses the inferior line and its corresponding muscle, terminating to the right and left of the line. I is a narrow muscle, whose head is fixed to the fold of the second division; the tail of it lying under n, and fastened to the edge of the skin that forms the cavity for the leg. The two muscles marked m have the same obliquity, and are placed the one on the other; the head is inserted in the skin under the muscle β, and communicates by a number of fibres with the tail of the muscle γ; the other end is fixed to the intermediate inferior line at the fold of the third division. The large and broad muscle n, covers the lower edge of the cavity of the limb, and the extremity of the tail of l. It is fixed first at the skin, near the intermediate line, from whence it goes in a perpendicular direction towards m, and introduces itself under o and m, where it is fixed. The muscle o is narrow and bent, and covers the edge of the cavity of the leg for a little way; one end terminating there, and the other finishing at the third division near m. That marked p is also bent: it runs near the anterior edge of the cavity of the leg; one end meets the head of o, the other end terminates at a raised fold near the inferior line. There is a triangular muscle on the side of the lateral muscle o, similar to that marked g in the following ring; in this figure it is entirely concealed by the muscle m.
The third ring has no muscle similar to m; that marked k differs only from that of the second ring in being crossed by the opposite muscle. Those marked l, n, o, p, are similar to those of the preceding one. The muscle q is triangular; the base is fastened to the last fold of the ring; on the lower side it is fixed to the muscle o, the top to the skin at the edge of the cavity for the leg. The eight following rings have the gastric muscles, \(i\), \(k\), \(l\), and \(m\). The muscle \(i\) is quite straight, and placed at some distance from the inferior line; it is broad at the fourth ring, but diminishes gradually in breadth to the fifth. In the fourth it is united; but divides into two heads, which diverge in the following rings. In the five next rings these heads are fixed nearly at the same place with \(a\) and \(f\); and in the other two it terminates at the fold of the ring. The anterior insertion of the first and last is at the fold where the ring begins; that of the five others is somewhat lower under the place where the muscle \(i\) terminates. The lower part of the oblique muscle \(k\) is inserted in the skin near \(i\); the upper part at the intermediate inferior muscle upon the fold which separates the following ring, but is wanting in the fifth. The muscle \(l\) is large, and co-operates with \(M\): in the opening and shutting the spiraculum, one of its fixtures is near the intermediate inferior line, at about the same height as \(i\). The tail terminates a little below the spiraculum.
The twelfth ring has only the single gastric muscle \(d\), which is a bundle of six, seven, or eight muscles; the first fixture of these is at the subdivision of the ring near the inferior line: one or two cross this, and at the same time the similar muscles of the opposite side. Their fixture is at the bottom of the foot; and their office is to assist the muscle \(a\) in bringing back the foot, and to loosen the claw from what it lays hold of. One of the insertions of this muscle \(a\) is observed in this figure near \(d\), the other near the subdivision of the ring.
Fig. 52, 53. Fig. 52, and 53, show the organization of the head of the coccus, though in a very imperfect manner, as M. Lyonet found it necessary to employ twenty figures to explain it fully. The head is represented as it appears when separated from the fat, and disengaged from the neck. HH are the two palpi. The truncated muscles D belong to the lower lip, and assist moving it. K shows the two ganglia of the neck united. II are the two vessels which assist in spinning the silk. L, the esophagus. M, the two dissolving vessels. The Hebrew characters נננ show the continuation of the four cephalic arteries. In fig. 52, the ten abductor muscles of the jaw are represented by SS, TT, VV, and Z. Four occipital muscles are seen in fig. 53, under e and f. At \(a\) is represented a nerve of the first pair belonging to the ganglion of the neck; \(b\) is a branch of this nerve.
Fig. 54. Exhibits the nerves as seen from the under part; but excepting in two or three nerves, which may be easily distinguished, only one of each pair is drawn, in order to avoid confusion. The nerves of the first ganglion of the neck are marked by capital letters, those of the ganglion (a) of the head by Roman letters; the nerves of the small ganglion by Greek characters. Those of the frontal ganglion, except one, by numbers.
The muscles of the coccus have neither the colour nor form of those of larger animals. In their natural state they are soft, and of the consistence of a jelly. Their colour is a grayish blue, which, with the silver-colored appearance of the pulmonary vessels, form a glorious spectacle. After the caterpillar has been soaked for some time in spirit of wine, they lose their elasticity and transparency, becoming firm, opaque, and white, and the air-vesicles totally disappear. The number of muscles in a caterpillar is very great. The greatest part of the head is composed of them, and there is a vast number about the esophagus, intestines, &c.; the skin is, as it were, lined by different beds of them, placed the one under the other, and ranged with great symmetry. M. Lyonet has been able to distinguish 228 in the head, 1647 in the body, and 2066 in the intestinal tube, making in all 3941.
At first sight the muscles might be taken for tendons, as being of the same colour, and having nearly the same lustre. They are generally flat, and of an equal size throughout; the middle seldom differing either in colour or size from either of the extremities. If they are separated, however, by means of very fine needles, in a drop of some fluid, we find them composed not only of fibres, membranes, and air-vesicles, but likewise of nerves; and, from the drops of oil that may be seen floating on the fluid, they appear also to be furnished with many unctuous particles. Their ends are fixed to the skin, but the rest of the muscle is generally free and floating. Several of them branch out considerably; and the branches sometimes extend so far, that it is not easy to discover whether they are distinct and separate muscles or parts of another. They are moderately strong; and those which have been soaked in spirit of wine, when examined by the microscope, are found to be covered with a membrane which may be separated from them; and they appear then to consist of several parallel bands lying longitudinally along the muscle, which, when divided by means of fine needles, appear to be composed of still smaller bundles of fibres lying in the same direction; which, when examined by a powerful magnifier, and in a favourable light, appear twisted like a small cord. The muscular fibres of the spider, which are much larger than those of the caterpillar, consist of two different substances, one soft and the other hard; the latter being twisted round the former spirally, and thus giving it the twisted appearance just mentioned.
There is nothing in the caterpillar similar to the brain in man. We find indeed in the head of this insect a part from which all the nerves seem to proceed; but this part is entirely unprotected, and so small, that it does not occupy one-fifth part of the head; the surface is smooth, and has neither lobes nor any anfractuosity like the human brain. But if we call this a brain in the caterpillar, we must say that it has thirteen: for there are twelve other such parts following each other in a straight line, all of them of the same substance with that in the head, and nearly of the same size; and from them, as well as from that in the head, the nerves are distributed through the body.
The spinal marrow in the coccus goes along the belly; is very small, forking out at intervals, nearly of the same thickness throughout, except at the ganglia, and is not enclosed in any case. It is by no means so tender as in man; but has a great degree of tenacity, and does not break without a considerable degree of tension. The substance of the ganglia differs from that of the spinal marrow, as no vesicles can be discovered in the latter; but the former The cossus has two large tracheal arteries, creeping under the skin close to the spiracula; one at the right and the other at the left side of the insect, each of them communicating with the air by means of nine spiracula. They are nearly as long as the whole caterpillar; beginning at the first spiraculum, and extending somewhat farther than the last; some branches also extending quite to the extremity of the body. Round each spiraculum the trachea pushes forth a great number of branches, which are again divided into smaller ones, and these further subdivided and spread through the whole body of the caterpillar. The tracheal artery, with all its numerous ramifications, are open elastic vessels, which may be pressed close together, or drawn out considerably, but return immediately to their usual size when the tension ceases. They are naturally of a silver colour, and make a beautiful appearance. This vessel, with its principal branches, is composed of three coats, which may be separated from one another. The outside is a thick membrane furnished with a great variety of fibres, which describe a vast number of circles round it, communicating with each other by numerous shoots. The second is very thin and transparent, without any particular vessel being distinguishable in it. The third is composed of fleshy threads, generally of a spiral form; and so near each other as scarcely to leave any interval. They are curiously united with the membrane which occupies the intervals; and form a tube which is always open, notwithstanding the flexure of the vessel. There are also many other peculiarities in its structure. The principal tracheal vessels divide into 1326 different branches.
The heart of the cossus is very different from that of larger animals, being almost as long as the animal itself. It lies immediately under the skin at the top of the back, entering the head, and terminating near the mouth. Towards the last rings of the body it is large and capacious, diminishing very much as it approaches the head, from the fourth to the twelfth division. On both sides, at each division, it has an appendage, which partly covers the muscles of the back, but which, growing narrower as it approaches the lateral line, it forms a number of irregular lozenge-shaped bodies.—This tube, however, seems to perform none of the functions of the heart in larger animals, as we find no vessel opening into it which answers either to the aorta or vena cava. It is called the heart, because it is generally filled with a kind of lymph, which naturalists have supposed to be the blood of the caterpillar; and because in all caterpillars which have a transparent skin, we may perceive alternate regular contractions and dilatations along the superior line, beginning at the eleventh ring, and proceeding from ring to ring, from the fourth; whence this vessel is thought to be a string or row of hearts. There are two white oblong bodies which join the heart near the eighth division; and these have been called reniform bodies, from their having somewhat of the shape of a kidney.
The most considerable part of the whole caterpillar with regard to bulk is the corpus cossum. It is the first and only substance that is seen on opening it. It forms a kind of sheath which envelopes and covers all Microscope, the entrails, and, introducing itself into the head, enters all the muscles of the body, filling the greatest part of the empty spaces in the caterpillar. It very much resembles the configuration of the human brain, and is of a milk-white colour.
The oesophagus descends from the bottom of the mouth to about the fourth division. The fore part, which is in the head, is flabby, narrow, and fixed by different muscles to the cranium parts of it; the lower part, which passes into the body, is wider, and forms a kind of membranaceous bag, covered with very small muscles; near the stomach it is narrower, and, as it were, confined by a strong nerve fixed to it at distant intervals. The ventricle begins a little above the fourth division, where the oesophagus ends, and finishes at the tenth. It is about seven times as long as broad; and the anterior part, which is broadest, is generally folded. These folds diminish with the bulk as it approaches the intestines; the surface is covered with a great number of aerial vessels, and opens into a tube, which M. Lyonet calls the large intestine.—There are three of these large tubes, each of which differs so much from the rest, as to require a particular name to distinguish it from them.
The two vessels from which the cossus spins its silk are often above three inches long, and are distinguished into three parts; the anterior, intermediate, and posterior. It has likewise two other vessels, which are supposed to prepare and contain the liquor for dissolving the wood on which it feeds.
Fig. 55 shows the wing of an earwig magnified; a represents it of the natural size. The wings of this insect are so artificially folded up under short cases, that few people imagine they have any. Indeed, they very rarely make use of their wings. The cases under which they are concealed are not more than a sixth part of the size of one wing, though a small part of the wing may be discovered, on a careful inspection, projecting from under them. The upper part of the wing is crustaceous and opaque, but the under part is beautifully transparent. In putting up their wings, they first fold back the parts A.B., and then shut up the ribs like a fan; the strong muscles used for this purpose being seen at the upper part of the figure. Some of the ribs are extended from the centre to the outer edge; others only from the edge about half way; but they are all united by a kind of band, at a small but equal distance from the edge; the whole evidently contrived to strengthen the wing, and facilitate its various motions. The insect itself differs very little in appearance in its three different states. De Geer affirms, that the female hatches eggs like a hen, and broods over her young ones as a hen does.
Fig. 56 represents a wing of the hemerobius perla magnified. It is an insect which seldom lives more than two or three days.—The wings are nearly of a length, and exactly similar to one another. They are composed of fine delicate nerves, regularly and elegantly disposed as in the figure, beautifully adorned with hairs, and lightly tinged with green. The body is of a fine green colour; and its eyes appear like two burnished beads of gold, whence it has obtained the name of golden eye. This insect lays its eggs on the leaves of the plum or the rose tree; the eggs are of a white colour, Microscope colour, and each of them fixed to a little pedicle or foot-stalk, by which means they stand off a little from the leaf, appearing like the fructification of some of the moths. The larva proceeding from these eggs resembles that of the coccinella or lady-cow, but is much more handsome. Like that, it feeds upon aphides or pucerons, sucking their blood, and forming itself a case with their dried bodies; in which it changes into the pupa state, from whence they afterwards emerge in the form of a fly.
Fig. E, F, I, represent the dust of a moth's wing magnified. This is of different figures in different moths. The natural size of these small plumes is represented at H.
Fig. 57 shows a part of the cornea of the libellula magnified. In some positions of the light, the sides of the hexagons appear of a fine gold colour, and divided by three parallel lines. The natural size of the part magnified is shown at b.
Fig. 58 shows the part e of a lobster's cornea magnified.
Fig. 59 shows one of the arms or horns of the lepas anatifa, or barnacle, magnified; its natural size being represented at d. Each horn consists of several joints, and each joint is furnished on the concave side of the arm with long hairs. When viewed in the microscope, the arms appear rather opaque; but they may be rendered transparent, and become a most beautiful object, by extracting out of the interior cavity a bundle of longitudinal fibres, which runs the whole length of the arm. Mr Needham thinks that the motion and use of these arms may illustrate the nature of the rotatory motion in the wheel-animal. In the midst of the arms is an hollow trunk, consisting of a jointed hairy-tube, which encloses a long round tongue that can be pushed occasionally out of the tube or sheath, and retracted occasionally. The mouth of the animal consists of six laminae, which go off with a bend, indented like a saw on the convex edge, and by their circular disposition are so ranged, that the teeth, in the alternate elevation and depression of each plate, act against whatever comes between them. The plates are placed together in such a manner, that to the naked eye they form an aperture not much unlike the mouth of a contracted purse.
Fig. 60 shows the apparatus of the tabanus or gadfly, by which it pierces the skin of horses and oxen, in order to suck the blood. The whole is contained in a fleety case, not expressed in the figure. The feelers aa are of a spongy texture and gray coloured, covered with short hairs. They are united to the head by a small joint of the same substance. They defend the other parts of the apparatus, being laid upon it side by side whenever the animal flings, and thus preserve it from external injury. The wound is made by the two lancets bb and B, which are of a delicate structure, but very sharp, formed like the dissecting knife of an anatomist, growing gradually thicker to the back.—The two instruments cc and C, appear as if intended to enlarge the wound, by irritating the parts round it; for which they are jagged or toothed. They may also serve, from their hard and horny texture, to defend the tube EE, which is of a softer nature, and tubular to admit the blood, and convey it to the stomach. This part is totally enclosed in a line DD, which entirely covers it. These parts are drawn separately at B, C, D, E, Microscopes De Geer observes, that only the females suck the blood of animals; and Reaumur informs us, that having made one, that had sucked its fill, digorger itself, the blood it threw up appeared to him to be more than the whole body of the insect could have contained. The natural size of this apparatus is shown at f.
Fig. 61 shows a bit of the skin of a lump-fish (Cyprinus) magnified. When a good specimen of this can be procured, it forms a most beautiful object. The tubercles exhibited in the figure probably secrete an unctuous juice.
Fig. 62 shows the scale of a sea perch found on the Fig. 63. English coast; the natural size is exhibited at h.
Fig. 63. the scale of an haddock magnified; its natural size as within the circle.
Fig. 64. the scale of a parrot fish from the West Indies magnified; / the natural size of it.
Fig. 65. the scale of a kind of perch in the West Indies magnified; k the natural size of the scale.
Fig. 66. part of the skin of a sole fish, as viewed through an opaque microscope; the magnified part, in its real size, shown at l.
The scales of fishes afford a great variety of beautiful objects for the microscope. Some are long; others round, square, &c., varying considerably not only in different fishes, but even in different parts of the same fish. Leeuwenhoek supposed them to consist of an infinite number of small scales or strata, of which those next to the body of the fish are the largest. When viewed by the microscope, we find some of them ornamented with a prodigious number of concentric flutings, too near each other, and too fine, to be easily enumerated. These flutings are frequently traversed by others diverging from the centre of the scale, and generally proceeding from thence in a straight line to the circumference.
For more full information concerning these and other microscopical objects, the reader may consult Mr Adams's Essays on the Microscope, who has made the most valuable collection that has yet appeared on the subject. See also the articles Animalcule, Crystallization, Polype, Plants, and Wood, in the present work.