ICROSCOPE, an optical instrument, consisting of lenses, or mirrors, by means of which small objects appear larger than they do to the naked eye. Single microscopes consist of a single lens or mirror; or if more lenses or mirrors be made use of, they only serve to throw light upon the object, but do not contribute to enlarge the image of it. Double or compound microscopes are those in which the image of an object is composed by means of more lenses or mirrors than one.
For the principles on which the construction of microscopes depends, see Optics. In the present article, it is intended to describe the finished instrument, with all its varied apparatus, according to the latest improvements; and to illustrate by proper details its uses and importance.
I. Of Single Microscopes.
The famous microscopes made use of by Mr Leeuwenhoek, were all, as Mr Baker assures us, of the single kind, and the construction of them was the most simple possible; each consisting only of a single lens set between two plates of silver, perforated with a small hole, with a moveable pin before it to place the object on and adjust it to the eye of the beholder. He informs us also, that lenses only, and not globules, were used in every one of these microscopes.
Plate 1. The single microscope now most generally known and used is that called Wilson's Pocket Microscope. The body is made of brass, ivory, or silver, and is represented by AA, BB. CC is a long fine threaded male screw that turns into the body of the microscope; D a convex glass at the end of the screw. Two concave round pieces of thin brass, with holes of different diameters in the middle of them, are placed to cover the above-mentioned glass, and thereby diminish the aperture when the greatest magnifiers are employed. EE, three thin plates of brass within the body of the microscope; one of which is bent semicircularly in the middle, so as to form an arched cavity for the reception of a tube of glass, the use of the other two being to receive and hold the sliders between them. F, a piece of wood or ivory, arched in the manner of the semicircular plate, and cemented to it. G, the other end of the body of the microscope, where a hollow female screw is adapted to receive the different magnifiers. H is a spiral spring of steel, between the end G and the plates of brass, intended to keep the plates in a right position and counteract the long screw CC. I is a small turned handle, for the better holding of the instrument, to screw on or off at pleasure.
To this microscope belong six or seven magnifying glasses: six of them are set in silver, brass, or ivory, as in the figure K; and marked 1, 2, 3, 4, 5, 6, the lowest numbers being the greatest magnifiers. L is the seventh magnifier, set in the manner of a little barrel, to be held in the hand for the viewing of any larger object. M is a flat slip of ivory, called a slider, with four round holes through it, wherein to place objects between two pieces of glass or Muscovy tale, as they appear at d d d d. Six such sliders, and one of brass, are usually sold with this microscope, some with objects placed in them, and others empty for viewing any thing that may offer: but whoever pleases to make a collection, may have as many as he desires. The brass slider is to confine any small object, that it may be viewed without crushing or destroying it. N is a tube of glass contrived to confine living objects, such as frogs, fishes, &c., in order to discover the circulation of the blood. All these are contained in a little neat box of fish-skin or mahogany, very convenient for carrying in the pocket.
When an object is to be viewed, thrust the ivory slider, in which the said object is placed, between the two flat brass plates EE: observing always to put that side of the slider where the brass rings are farthest from the eye. Then screw on the magnifying glass you intend to use, at the end of the instrument G; and looking through it against the light, turn the long screw CC, till your object be brought to suit your eye; which will be known by its appearing perfectly distinct and clear. It is most proper to look at it first through a magnifier that can show the whole at once, and afterwards to inspect the several parts more particularly with one of the greatest magnifiers; for thus you will gain a true idea of the whole, and of all its parts. And though the greatest magnifiers can show but a minute portion of any object at once, such as the claw of a flea, the horn of a louse, or the like; yet by gently moving the slider which contains the object, the eye may gradually examine it all over.
As objects must be brought very near the glasses when the greatest magnifiers are made use of, be careful not to scratch them by rubbing the slider against them. them as you move it in or out. A few turns of the screw CC will easily prevent this mischief, by giving them room enough. You may change the objects in your sliders for any others you think proper, by taking out the brass rings with the point of a penknife; the tales will then fall out, if you but turn the sliders; and after putting what you please between them, by replacing the brass rings you will fasten them as they were before. It is proper to have some sliders furnished with tales, but without any object between them, to be always in readiness for the examination of fluids, salts, sands, powders, the farina of flowers, or any other casual objects of such sort as need only be applied to the outside of the tale.
The circulation of the blood may be easiest seen in the tails or fins of fishes, in the fine membranes between a frog's toes, or best of all in the tail of a water-newt. If your object be a small fish, place it within the tube N, and spread its tail or fin along the side thereof: if a frog, choose such a one as can but just be got into your tube; and, with a pen, or small stick, expand the transparent membrane between the toes of the frog's hind foot as much as you can. When your object is so adjusted that no part of it can intercept the light from the place you intend to view, unscrew the long screw CC, and thrust your tube into the arched cavity, quite through the body of the microscope; then screw it to the true focal distance, and you will see the blood passing along its vessels with a rapid motion, and in a most surprising manner.
The third or fourth magnifiers may be used for frogs or fishes: but for the tails of water-newts, the fifth or sixth will do; because the globules of their blood are twice as large as those of frogs or fish. The first or second magnifier cannot well be employed for this purpose; because the thickness of the tube in which the object lies, will scarce admit its being brought so near as the focal distance of the magnifier.
An apparatus for the purpose of viewing opaque objects generally accompanies this microscope; and which consists of the following parts. A brass arm QR, which is screwed at Q, upon the body of the microscope at G. Into the round hole R, any of the magnifiers suitable to the object to be viewed are to be screwed; and under it, in the same ring, the concave polished silver speculum S. Through a small aperture in the body of the microscope under the brass plates EE, is to slide the long wire with the forceps T: This wire is pointed at one of its ends; and so, that either the points or forceps may be used for the objects as may be necessary. It is easy to conceive, therefore, that the arm at R, which turns by a twofold joint at a and b, may be brought with its magnifier over the object, the light reflected upon it by the application of the speculum, and the true focus obtained by turning of the male screw CC as before directed.—As objects are sometimes not well fixed for view, either by the forceps or point, the small piece shown at V is added, and in such cases answers better: it screws over the point of T; it contains a small round piece of ivory, blackened on one side, and left white upon the other as a contrast to coloured objects, and by a small piece of watch-spring fastens down the objects upon the ivory.
2. Single Microscope by reflection. In fig. 2. A is a Microscroll of brass fixed upright upon a round wooden base B, or a mahogany drawer or case, so as to stand perfectly firm and steady. C is a brass screw, that passes through a hole in the upper limb of the scroll into the side of the microscope D, and screws it fast to the said scroll. E is a concave speculum set in a box of brass, which hangs in the arch G by two small screws ff, that screw into the opposite sides thereof. At the bottom of this arch is a pin of the same metal, exactly fitted to a hole h in the wooden pedestal, made for the reception of the pin. As the arch turns on this pin, and the speculum turns on the end of the arch, it may, by this twofold motion, be easily adjusted in such a manner as to reflect the light of the sun, of the sky, or of a candle, directly upwards through the microscope that is fixed perpendicularly over it; and by so doing may be made to answer many purposes of the large double reflecting microscope. The body of the microscope may also be fixed horizontally, and objects viewed in that position by any light you choose; which is an advantage the common double reflecting microscope has not. It may also be rendered further useful by means of a slip of glass, one end of which being thrust through between the plates where the sliders go, and the other extending to some distance, such objects may be placed thereon as cannot be applied in the sliders: and then, having a limb of brass that may fasten to the body of the microscope, and extend over the projecting glass a hollow ring wherein to screw the magnifiers, all sorts of subjects may be examined with great convenience, if a hole be made in the pedestal, to place the speculum exactly underneath, and thereby throw up the rays of light. The pocket-microscope, thus mounted, says Mr Baker, "is as easy and pleasant in its use; as fit for the most curious examination of the animalcules and salts in fluids, of the farine in vegetables, and of the circulation in small animals; in short, is as likely to make considerable discoveries in objects, that have some degree of transparency, as any microscope I have ever seen or heard of."
The brass scroll A is now generally made to unscrew into three parts, and pack with the microscope and apparatus into the drawer of a mahogany pocket-case, upon the lid of which the scroll is made to fix when in use.
The opaque apparatus also, as above described, is applicable this way by reflection. It only consists in turning the arm R (fig. 1.), with the magnifier over the concave speculum below (fig. 2.), or to receive the light as reflected obliquely from it: the silver speculum screwed into R will then reflect the light, which it receives from the glass speculum, strongly upon the object that is applied upon the wire T underneath.
This microscope, however, is not upon the most convenient construction, in comparison with others now made: it has been esteemed for many years past from its popular name, and recommendation by its makers. Its portability is certainly a great advantage in its favour; but in most respects it is superseded by the microscope hereafter described.
3. Microscope for Opaque Objects, called the Single Opaque Microscope. This microscope remedies the inconvenience of having the dark side of an object next the eye, which formerly was an insurmountable objection to the making observations on opaque objects with any considerable degree of exactness or satisfaction; for, in all other contrivances commonly known, the nearness of the instrument to the object (when glasses that magnify much are used) unavoidably overshadows it so much, that its appearance is rendered obscure and indistinct. And notwithstanding ways have been tried to point light upon an object, from the sun or a candle, by a convex glass placed on the side thereof, the rays from either can be thrown upon it in such an acute angle only, that they serve to give a confused glare, but are insufficient to afford a clear and perfect view of the object. But in this microscope, by means of a concave speculum of silver highly polished, in whose centre a magnifying lens is placed, such a strong and direct light is reflected upon the object, that it may be examined with all imaginable ease and pleasure. The several parts of this instrument, made either of brass or silver, are as follow.
Through the first side A, passes a fine screw B, the other end of which is fastened to the moveable side C. D is a nut applied to this screw, by the turning of which the two sides A and C are gradually brought together. E is a spring of steel that separates the two sides when the nut is unscrewed. F is a piece of brass, turning round in a socket, whence proceeds a small spring tube moving upon a rivet; through which tube there runs a steel wire, one end whereof terminates in a sharp point G, and the other with a pair of pliers H fastened to it. The point and pliers are to thrust into, or take up and hold, any insect or object; and either of them may be turned upwards, as best suits the purpose. I is a ring of brass, with a female screw within it, mounted on an upright piece of the same metal; which turns round on a rivet, that it may be set at a due distance when the least magnifiers are employed. This ring receives the screws of all the magnifiers. K is a concave speculum of silver, polished as bright as possible; in the centre of which is placed a double convex lens, with a proper aperture to look through it. On the back of this speculum a male screw L is made to fit the brass ring I, to screw into it at pleasure. There are four of these concave specula of different depths, adapted to four glasses of different magnifying powers, to be used as the objects to be examined may require. The greatest magnifiers have the least apertures. M is a round object-plate, one side of which is white and the other black: The intention of this is to render objects the more visible, by placing them, if black, on the white side, or, if white, on the black side. A steel spring N turns down on each side to make any object fast; and issuing from the object-plate is a hollow pipe to screw it on the needle's point G. O is a small box of brass, with a glass on each side, contrived to confine any living object, in order to examine it: this also has a pipe to screw upon the end of the needle G. P is a turned handle of wood, to screw into the instrument when it is made use of. Q, a pair of brass pliers to take up any object, or manage it with convenience. R is a soft hair-brush for cleaning the glasses, &c. S is a small ivory box for tales, to be placed, when wanted, on the small brass box O.
When you would view any object with this micro-
scope, screw the speculum, with the magnifier you think proper to use, into the brass ring I. Place your object, either on the needle G in the pliers H, on the object-plate M, or in the hollow brass box O, as may be most convenient; then holding up your instrument by the handle P, look against the light through the magnifying lens; and by means of the nut D, together with the motion of the needle, by managing its lower end, the object may be turned about, raised, or depressed, brought nearer the glass, or removed farther from it, till you find the true focal distance, and the light be seen strongly reflected from the speculum upon the object, by which means it will be shown in a manner surprisingly distinct and clear; and for this purpose the light of the sky or of a candle will answer very well. Transparent objects may also be viewed by this microscope; only observing, that when such come under examination, it will not always be proper to throw on them the light reflected from the speculum; for the light transmitted through them, meeting the reflected light, may together produce too great a glare. A little practice, however, will show how to regulate both lights in a proper manner.
4. Ellis's single and Aquatic Microscope. Fig. 4. re-Fig. 4 presents a very convenient and useful microscope, contrived by Mr John Ellis, author of An Essay upon Corallines, &c. To practical botanists, observers of animals, &c., it possesses many advantages above those just described. It is portable, simple in its construction, expeditious, and commodious in use. K represents the box containing the whole apparatus: it is generally made of fish-skin; and on the top there is a female screw, for receiving the screw that is at the bottom of the pillar A: this is a pillar of brass, and is screwed on the top of the box. D is a brass pin which fits into the pillar; on the top of this pin is a hollow socket to receive the arm which carries the magnifiers; the pin is to be moved up and down, in order to adjust the lenses to their focal or proper distance from the object. [N.B. In the representations of this microscope, the pin D is delineated as passing through a socket at one side of the pillar A; whereas it is usual at present to make it pass down a hole bored through the middle of the pillar.] E, the bar which carries the magnifying lens; it fits into the socket X, which is at the top of the pin or pillar D. This arm may be moved backwards and forwards in the socket X, and sideways by the pin D; so that the magnifier, which is screwed into the ring at the end E of this bar, may be easily made to traverse over any part of the object that lies on the stage or plate B. FF is a polished silver speculum, with a magnifying lens placed at the centre thereof, which is perforated for this purpose. The silver speculum screws into the arm E, as at F. G, another speculum, with its lens, which is of a different magnifying power from the former. H, the semicircle which supports the mirror I; the pin R, affixed to the semicircle H, passes through the hole which is towards the bottom of the pillar A. B, the stage, or the plane, on which the objects are to be placed; it fits into the small dove-tailed arm which is at the upper end of the pillar DA. C, a plane glass, with a small piece of black silk stuck on it; this glass is to lay in a groove made in the stage C. M, a hollow glass to be laid occasionally on the stage in- stead of the plane glass C. L., a pair of nippers. These are fixed to the stage by the pin at bottom; the steel wire of these nippers slides backwards and forwards in the socket, and this socket is moveable upwards and downwards by means of the joint, so that the position of the object may be varied at pleasure. The object may be fixed in the nippers, stuck on the point, or affixed, by a little gum-water, &c. to the ivory cylinder N, which occasionally screws to the point of the nippers.
To use this microscope: Take all the parts of the apparatus out of the box; then begin by screwing the pillar A to the cover thereof; pass the pin R of the semicircle which carries the mirror through the hole that is near the bottom of the pillar A; push the stage into the dove-tail at B; slide the pin into the pillar (see the N. B. above); then pass the bar E through the socket which is at the top of the pin D, and screw one of the magnifying lenses into the ring at F. The microscope is now ready for use: and though the enumeration of the articles may lead the reader to imagine the instrument to be of a complex nature, we can safely affirm that he will find it otherwise. The instrument has this peculiar advantage, that it is difficult to put any of the pieces in a place which is appropriated to another. Let the object be now placed either on the stage or in the nippers L, and in such manner that it may be as nearly as possible over the centre of the stage; bring the speculum F over the part you mean to observe; then throw as much light on the speculum as you can, by means of the mirror I, and the double motion of which it is capable; the light received on the speculum is reflected by it on the object. The distance of the lens F from the object is regulated by moving the pin D up and down, until a distinct view of it is obtained. The best rule is, to place the lens beyond its focal distance from the object, and then gradually to slide it down till the object appears sharp and well defined. The adjustment of the lenses to their focus, and the distribution of the light on the object, are what require the most attention: on the first the distinctness of the vision depends; the pleasure arising from a clear view of the parts under observation is due to the modification of the light. No precise rule can be given for attaining accurately these points; it is from practice alone that ready habits of obtaining these necessary properties can be acquired, and with the assistance of this no difficulty will be found.
5. A very simple and convenient microscope for botanical and other purposes, though inferior in many respects to that of Mr Ellis, was contrived by the ingenious Mr Benjamin Martin, and is represented at fig. 5, where AB represents a small arm supporting two or more magnifiers, one fixed to the upper part as at B, the other to the lower part of the arm at C; these may be used separately or combined together. The arm AB is supported by the square pillar IK, the lower end of which fits into the socket E of the foot FG; the stage DL is made to slide up and down the square pillar; H, a concave mirror for reflecting light on the object.—To use this microscope, place the object on the stage, reflect the light on it from the concave mirror, and regulate it to the focus, by moving the stage nearer to or farther from the lens at B. The ivory sliders pass through the stage; other objects may be fixed in the nippers MN, and then brought under the eye-glasses; or they may be laid on one of the glasses which fit the stage. The apparatus to this instrument consists of three ivory sliders; a pair of nippers; a pair of forceps; a flat glass and a concave ditto, both fitted to the stage.
The two last microscopes are frequently fitted up with a toothed rack and pinion, for the more ready adjustment of the glasses to their proper focus.
6. Withering's portable Botanic Microscope. Fig. 6. Fig. 6 represents a small botanical microscope contrived by Dr Withering, and described by him in his Botanical Arrangements. It consists of three brass plates, ABC, which are parallel to each other; the wires D and E are riveted into the upper and lower plates, which are by this means united to each other; the middle plate or stage is moveable on the aforesaid wires by two little sockets which are fixed to it. The two upper plates each contain a magnifying lens, but of different powers; one of these confines and keeps in their places the fine point F, the forceps G, and the small knife H.—To use this instrument, unscrew the upper lens, and take out the point, the knife, and the forceps; then screw the lens on again, place the object on the stage, and then move it up or down till you have gained a distinct view of the object, as one lens is made of a shorter focus than the other; and spare lenses of a still deeper focus may be had if required. This little microscope is the most portable of any. Its principal merit is its simplicity.
7. Botanical Lenses or Magnifiers. The haste with which botanists, &c. have frequently occasion to view objects, renders an extempore pocket-glass indispensably necessary. The most convenient of any yet constructed, appears to be that contrived, in regard to the form of the mounting, by Mr Benjamin Martin; and is what he called a Hand Megaloscope, because it is well adapted for viewing all the larger sort of small objects universally, and by only three lenses it has seven different magnifying powers.
Fig. 7. represents the case with the three frames and lenses, which are usually of 1, 1½, and 2 inches focus; they all turn over each other, and shut into the case, and are turned out at pleasure.
The three lenses singly, afford three magnifying powers; and by combining two and two, we make three more: for d with e makes one, d with f another, and e with f a third; which, with the three singly, make six; and lastly, all three combined together make another; so that upon the whole, there are seven powers of magnifying with these glasses only.
When the three lenses are combined, it is better to turn them in, and look through them by the small apertures in the sides of the case. The eye in this case is excluded from extra light; the aberration of the superfluous rays through the glasses is cut off; and the eye coincides more exactly with the common axes of the lenses.
A very useful and easy kind of microscope (described by Joblet, and which has been long in use), adapted chiefly for viewing, and confining at the same time, any living insects, small animals, &c. is shown at fig. 8. Fig. 8, where A represents a glass tube, about 1½ inch diameter, and 2 inches high. B, a case of brass or wood, containing a sliding tube, with two or three magnify- ing glasses that may be used either separately or combined. In the inside, at the bottom, is a piece of ivory, black and white on opposite sides, that is occasionally removed, and admits a point to be screwed into the centre. The cap unscrews at D, to admit the placing of the object; the proper distance of the glasses from the object is regulated by pulling up or down the brass tube E at top containing the eye-glasses.
This microscope is particularly useful for exhibiting the well-known curious curculio imperialis, vulgarly called the diamond beetle, to the greatest advantage; for which, as well as for other objects, a glass bottom, and a polished reflector at the top, are often applied, to condense the light upon the object. In this case, the stand and brass-bottom F, as shown in the figure, are taken away by unscrewing.
9. Mr Lyonet's Single Anatomical Dissecting Microscope.—Fig. 9 represents a curious and extremely useful microscope, invented by that gentleman for the purpose of minute dissections, and microscopic preparations. This instrument must be truly useful to amateurs of the minutiae of insects, &c., being the best adapted of any for the purposes of dissection. With this instrument Mr Lyonet made his very curious microscopical dissection of the chenille de saule, as related in his Traité Anatomique de la chenille qui rouge le bois de saule, &c.
AB is the anatomical table, which is supported by a pillar NO; this is screwed on the foot CD. The table AB is prevented from turning round by means of two steady pins. In this table or board there is a hole G, which is exactly over the centre of the mirror EF, that is to reflect the light on the object; the hole G is designed to receive a flat or concave glass, on which the objects for examination are to be placed.
RXZ is an arm formed of several balls and sockets, by which means it may be moved in every possible situation; it is fixed to the board by means of the screw H. The last arm IZ has a female screw, into which a magnifier may be screwed as at Z. By means of the screw H, a small motion may be occasionally given to the arm IZ, for adjusting the lens with accuracy to its focal distance from the object.
Another chain of balls is sometimes used, carrying a lens to throw light upon the object; the mirror is likewise so mounted, as to be taken from its place at K, and fitted on a clamp, by which it may be fixed to any part of the table AB.
To use the Dissecting Table.—Let the operator sit with his left side near a light window; the instrument being placed on a firm table, the side DH towards the stomach, the observations should be made with the left eye. In dissecting, the two elbows are to be supported by the table on which the instrument rests, the hands resting against the board AB; and in order to give it greater stability (as a small shake, though imperceptible to the naked eye, is very visible in the microscope), the dissecting instruments are to be held one in each hand, between the thumb and two fore-fingers.
II. Of Double Microscopes, commonly called Compound Microscopes.
Double microscopes are so called, from being a combination of two or more lenses.
The particular and chief advantages which the compound microscopes have over the single, are, that the objects are represented under a larger field of view, and with a greater amplification of reflected light.
1. Culpeper's Microscope.—The compound microscope, originally contrived by Mr Culpeper, is represented at fig. 10. It consists of a large external brass body A, B, C, D, supported upon three scrolls, which are fixed to the stage EF; the stage is supported by three larger scrolls, that are screwed to the mahogany pedestal GH. There is a drawer in the pedestal, which holds the apparatus. The concave mirror I is fitted to a socket in the centre of the pedestal. The lower part LMCD of the body forms an exterior tube, into which the upper part of the body ABLM slides, and may be moved up or down, so as to bring the magnifiers, which are screwed on at N, nearer to or farther from the object.
To use this microscope: Screw one of the buttons, which contains a magnifying lens, to the end N of the body; place the slider, with the objects, between the plates of the slider-holder. Then, to attain distinct vision, and a pleasing view of the object, adjust the body to the focus of the lens you are using, by moving the upper part gently up and down, and regulate the light by the concave mirror.
For opaque objects, two additional pieces must be used. The first is a cylindrical tube of brass (represented at L, fig. 11.), which fits on the cylindrical part at N of the body. The second piece is the concave speculum h; this is to be screwed to the lower end of the aforesaid tube; the upper edge of this tube should be made to coincide with the line which has the same number affixed to it as to the magnifier you are using; e.g., if you are making use of the magnifier marked 5, slide the tube to the circular line on the tube N that is marked also with No 5. The slider-holder should be removed when you are going to view opaque objects, and a plane glass should be placed on the stage in its stead to receive the object; or it may be placed in the nippers, the pin of which fits into the hole in the stage.
The apparatus belonging to this microscope consists of the following particulars: viz. Five magnifiers, each fitted in a brass button; one of these is seen at N, fig. 10. Six ivory sliders, five of them with objects. A brass tube to hold the concave speculum. The concave speculum in a brass box. A fish pan. A set of glass tubes. A flat glass fitted to the stage. A concave glass fitted to the stage. A pair of forceps. A steel wire, with a pair of nippers at one end and a point at the other. A small ivory cylinder, to fit on the pointed end of the aforesaid nippers. A convex lens, moveable in a brass semicircle; this is affixed to a long brass pin, which fits into a hole on the stage.
The construction of the foregoing microscope is very simple, and it is easy in use; but the advantages of the stage and mirror are too much confined for an extensive application and management of all kinds of objects. Its greatest recommendation is its cheapness; and to those who are desirous of having a compound microscope at a low price, it may be acceptable.
2. Cuff's Microscope.—The improved microscope next in order is that of Mr Cuff. Besides remedying the disadvantages above mentioned, it contains the addition... addition of an adjusting screw, which is a considerable improvement, and highly necessary to the examination of objects under the best defined appearance from the glasses. It is represented at fig. ii. with the apparatus that usually accompanies it. A, B, C, shows the body of this microscope; which contains an eye-glass at A; a broad lens at B, and a magnifier which is screwed on at C. The body is supported by the arm DE, from which it may be removed at pleasure. The arm DE is fixed on the sliding bar F, and may be raised or depressed to any height within its limits. The main pillar a b is fixed in the box b c; and by means of the brass foot d is screwed to the mahogany pedestal XY, in which is a drawer containing all the apparatus. O is a milled-headed screw, to tighten the bar F when the adjusting screw e g is used. p q Is the stage, or plate, which carries the objects; it has a hole at the centre v. G, a concave mirror, that may be turned in any direction, to reflect the light of a candle, or the sky, upon the object.
To use this microscope: Screw the magnifier you intend to use to the end C of the body; place the slider-holder P in the hole n, and the slider with the object between the plates of the slider-holder; set the upper edge of the bar DE to coincide with the divisions which correspond to the magnifier you have in use, and pinch it by the milled nut; now reflect a proper quantity of light upon the object, by means of the concave mirror G, and regulate the body exactly to the eye and the focus of the glasses by the adjusting screw e g.
To view opaque objects, take away the slider-holder P, and place the object on a flat glass under the centre of the body, or on one end of the jointed nippers o p. Then screw the silver concave speculum h to the end of the cylinder L, and slide this cylinder on the lower part of the body, so that the upper edge thereof may coincide with the line which has the same mark with the magnifier that is then used: reflect the light from the concave mirror G to the silver speculum, from which it will again be reflected on the object. The glasses are to be adjusted to their focal distance as before directed.
The apparatus consists of a convex lens H, to collect the rays of light from the sun or a candle, and condense them on the object. L a cylindrical tube, open at each side, with a concave speculum screwed to the lower end h. P the slider-holder: this consists of a cylindrical tube, in which an inner tube is forced upwards by a spiral spring; it is used to receive an ivory slider K, which is to be slid between the plates h and i. The cylinder P fits the hole n in the stage; and the hollow part at k is designed to receive a glass tube. R is a brass cone, to be put under the bottom of the cylinder P, to intercept occasionally some of the rays of light. S, a box containing a concave and a flat glass, between which a small living insect may be confined: it is to be placed over the hole n. T, a flat glass, to lay any occasional object upon; there is also a concave one for fluids. O is a long steel wire, with a small pair of pliers at one end, and a point at the other, designed to stick or hold objects: it slips backwards and forwards in the short tube o; the pin p fits into the hole of the stage. W, a little round ivory box, to hold a supply of talc and rings for the Microscope. V, a small ivory cylinder, that fits on the pointed end of the steel wire: it is designed for opaque objects. Light-coloured ones are to be stuck upon the dark side, and vice versa. M, a fish-pan, whereon to fasten a small fish, to view the circulation of the blood: the tail is to be spread across the oblong hole k at the small end, and tied fast, by means of a ribbon fixed thereto; the knob l is to be shoved through the slit made in the stage, that the tail may be brought under the magnifier.
3. This microscope has received several material improvements from Mr Martin, Mr Adams, &c. By an alteration, or rather an enlargement, of the body of the tube which contains the eye-glasses, and also of the eye-glasses themselves, the field of view is made much larger; the mirror below for reflecting light is made to move upon the same bar with the stage; by which means the distance of it from the stage may be very easily and suitably varied. A condensing glass is applied under the stage in the slider-holder, in order to modify and increase the light that is reflected by the mirrors below from the light of a candle or lamp. It is furnished also with two mirrors in one frame, one concave and the other plane, of glass silvered; and by simply unscrewing the body, the instrument, when desired, may be converted into a single microscope. Fig. 12. is a representation of the instrument thus improved; and the following is the description of it, as given by Mr Adams in his Essays.
AB represents the body of the microscope, containing a double eye-glass and a body-glass; it is here shown as screwed to the arm CD, from whence it may be occasionally removed, either for the convenience of packing, or when the instrument is to be used as a single microscope.
The eye-glasses and the body-glasses are contained in a tube which fits into the exterior tube AB; by pulling out a little this tube when the microscope is in use, the magnifying power of each lens is increased.
The body AB of the microscope is supported by the arm CD; this arm is fixed to the main pillar CF, which is screwed firmly to the mahogany pedestal GH; there is a drawer to this pedestal, which holds the apparatus.
(fig. 24.) represents the body of the solar microscope; one part thereof, ABCD, is conical; the other, CDEF, is cylindrical. The cylindrical part receives the tube G of the opaque box, or the tube Y of the single microscope. At the large end AB of the conical part, there is a lens to receive the rays from the mirror, and refract them towards the box HIKL. NOP is a brass frame; which is fixed to the moveable circular plate a b c; in this frame there is a plane mirror, to reflect the solar rays on the aforementioned lens. This mirror may be moved into the most convenient position for reflecting the light, by means of the nuts Q and R. By the nut Q it may be moved from east to west; and it may be elevated or depressed by the nut R. d e Two screws to fasten the microscope to a window shutter. The box for opaque objects is represented at HIKL; it contains a plane mirror M, for reflecting the light which it receives from the large lens to the object, and thereby illuminating it; S is a screw to adjust this mirror, or place it at a proper angle for reflecting the light. VX, two tubes of brass, one sliding within the other, the exterior one in the box HIKL; these carry the magnifying lenses: the interior tube is sometimes taken out, and the exterior one is then used by itself. Part of this tube may be seen in the plate within the box HIKL. At H there is a brass plate, the back part of which is fixed to the hollow tube A, in which there is a spiral wire, which keeps the plate always bearing against the side H of the brass box HIKL. The sliders, with the opaque objects, pass between this plate and the side of the box; to put them there, the plate is to be drawn back by means of the nut g; i k is a door to one side of the opaque box. The foregoing pieces constitute the several parts necessary for viewing opaque objects. We shall now proceed to describe the single microscope, which is used for transparent objects: but in order to examine these, the box HIKL must be first removed, and in its place we must insert the tube Y of the single microscope that we are now going to describe.
Fig. 25. represents a large tooth and pinion microscope: at m, within the body of this microscope, are two thin plates, that are to be separated in order to let the ivory sliders pass between them; they are pressed together by a spiral spring, which bears up the under plate, and forces it against the upper one.
The slider B (under fig. 4.), which contains the magnifiers, fits into the hole n; and any of the magnifiers may be placed before the object, by moving the aforesaid slider; when the magnifier is at the centre of the hole P, a small spring falls into one of the notches which is on the side of the slider.
Under the plate m are placed two lenses, for enlarging the field of view on the screen: the smaller of the two is fixed in a piece of brass, and is nearest the plate m; this is to be taken out when the magnifiers, No 4, 5, or 6, are used, or when the megascopic lens T (fig. 24.) is used; but is to be replaced for No 1, 2, 3.
This microscope is adjusted to the focus by turning the milled nut O.
To use the solar microscope:—Make a round hole in the window shutter, a little larger than the circle a b c; pass the mirror ONP through this hole, and apply the square plate to the shutter; then mark with a pencil the places which correspond to the two holes through which the screw is to pass; take away the microscope, and bore two holes at the marked places, sufficiently large to let the milled screws d e pass through them.
The screws are to pass from the outside of the shutter, to go through it: and being then screwed into their respective holes in the square plate, they will, when screwed home, hold it fast against the inside of the shutter, and thus support the microscope.
Screw the conical tube ABCD to the circle a b c, and then slide the tube G of the opaque box into the cylindrical part CDEF of the body, if opaque objects are to be examined; but if they be transparent objects you mean to show, then place the tube Y within the tube CDEF.
The room is to be darkened as much as possible, that no light may enter but what passes through the body of the microscope; for, on this circumstance, together with the brightness of the sunshine, the perfection and distinctness of the image in a great measure depend.
When the microscope is to be used for opaque objects, 1. Adjust the mirror NOP, so as to receive the solar rays, by means of the two finger screws or nuts, QR; the first, Q, turns the mirror to the right or left; the second, R, raises or depresses it: this you are to do till you have reflected the sun’s light through the lens at AB strongly upon a screen of white paper placed at some distance from the window, and formed thereon a round spot of light. An unexperienced observer will find it more convenient to obtain the light by forming this spot before he puts on either the opaque box or the tooth and pinion microscope.
Now put in the opaque box, and place the object between the plates at H; open the door i k, and adjust the mirror M till you have illuminated the object strongly. If you cannot effect this by the screw S, you must move the screws Q, R, in order to get the light reflected strongly from the mirror NOP, or the mirror mirror M, without which the latter cannot illuminate the object.
The object being strongly illuminated, shut the door i k; and a distinct view of the object will soon be obtained on your screen, by adjusting the tubes VX, which is effected by moving them backwards or forwards.
A round spot of light cannot always be procured in northern latitudes, the altitude of the sun being often too low; neither can it be obtained when the sun is directly perpendicular to the front of the room.
he sun is continually changing its place, it will be necessary, in order to keep his rays full upon the object, to keep them continually directed through the axis of the instrument, by the two screws Q and R.
To view transparent objects, remove the opaque box, and insert the tube Y, fig. 25, in its place; put the slider S into its place at m, and the slider with the objects between the plates at m; then adjust the mirror NOP, as before directed by the screws Q, R, so that the light may pass through the object; regulate the focus of the magnifier by the screw O. The most pleasing magnifiers in use are the fourth and fifth.
The size of the object may be increased or diminished, by altering the distance of the screen from the microscope; five or six feet is a convenient distance.
To examine transparent objects of a larger size, or to render the instrument what is usually called a megulascope, take out the slider S from its place at n, and screw the button T (fig. 24.) into the hole at P, fig. 25, and remove the glass which is under the plate at m, and regulate the light and focus agreeable to the foregoing directions.
N.B. At the end of the tube G there is a lens for increasing the density of the rays, for the purpose of burning or melting any combustible or fusible substance; this lens must be removed in most cases, lest the objects should be burnt. The intensity of the light is also varied by moving this tube backwards or forwards.
f the Opaque Solar Microscope.—The large square plate and mirror; the body of the microscope; the opaque box and its tube; the tooth and pinion microscope; the slider with the magnifiers; the megulascope magnifier; the two screws d and e; some ivory sliders; some sliders with opaque objects; a brass frame, with a bottom of soft deal to stick any object on; a brass cylinder K (fig. 31.), for confining opaque objects.
IV. The Camera Obscura, or Lucernal Microscope.
The great facility with which objects can be represented on paper or a rough glass in the camera obscura, and copies drawn from them by any person though unskilled in drawing, evidently suggested the application of the microscope to this instrument. The greatest number of experiments that appear to have been made with this view, were by Mr Martin and Mr Adams; the former of whom frequently applied the microscope to the portable camera, and with much effect and entertainment. But these instruments being found to answer only with the assistance of the sun, Mr Adams directed his experiments to the construction of an instrument of more extended utility, which could be equally employed in the day-time and by night. He accordingly succeeded so far as to produce, by candle-light, the images of objects refracted from a single magnifier upon one or two large convex lenses (of about five inches or upwards in diameter), at the end of a pyramidal shaped box, in a very pleasing and magnified appearance, so as to give opaque objects as well as transparent ones the utmost distinctness of representation; but still the light of a candle or lamp was found generally insufficient to throw the requisite degree of illumination upon the objects. The invention of what is called Argand's lamp, within these few years, offered a complete remedy for this defect, by the intensity and steadiness of its light. This did not escape Mr Adams (son of the former), who immediately applied it; and who had likewise so altered and improved his father's instrument, both in construction and form, as to render it altogether a different one, and far more perfect and useful.
The advantages and properties of this excellently conceived instrument are numerous and important. As the far greater part of the objects which surround us are opaque (says our author), and very few are sufficiently transparent to be examined by the common microscopes, an instrument that could be readily applied to the examination of opaque objects has always been a desideratum. Even in the examination of transparent objects, many of the fine and more curious portions are lost, and drowned, as it were, in the light which must be transmitted through them; while different parts of the same object appear only as dark lines or spots, because they are so opaque as not to permit any light to pass through them. These difficulties, as well as many more, are obviated in the lucernal microscope; by which opaque objects of various sizes may be seen with ease and distinctness: the beautiful colours with which most of them are adorned, are rendered more brilliant, without changing in the least the real tint of the colour; and the concave and convex parts retain also their proper form.—The facility with which all opaque objects are applied to this instrument, is another considerable advantage, and almost peculiar to itself; as the texture and configuration of the more tender parts are often hurt by previous preparation, every object may be examined by this instrument, first as opaque, and afterwards (if the texture will admit of it) as transparent.—The lucernal microscope does not in the least fatigue the eye; the object appears like nature itself, giving ease to the sight and pleasure to the mind: there is also, in the use of this instrument, no occasion to shut the eye which is not directed to the object. A further advantage peculiar to this microscope is, that by it the outlines of every object may be taken, even by those who are not accustomed to draw; while those who can draw well will receive great assistance, and execute their work with more accuracy and in less time than they would otherwise have been able to have performed it. Transparent objects as well as opaque may be copied in the same manner. The instrument may be used at any time of the day, but the best effect is by night; in which respect it has a superiority over the solar microscope, as that instrument can only be used when the sun shines.
Transparent objects may be examined with the lucernal microscope in three or four different modes, from a blaze of light almost too great for the eye to bear, to that which is perfectly easy to it: And by the addition of a tin lanthorn to the apparatus, may be thrown on a screen, and exhibited at one view to a large company, as by the solar microscope.
We shall now proceed to the description of the instrument and apparatus as given by Mr Adams.
Fig. 26 represents the improved Lucernal Microscope, mounted to view opaque objects. ABCD is a large mahogany pyramidal box, which forms the body of the microscope; it is supported firmly on the brass pillar FG, by means of the socket H and the curved piece IK.
LMN is a guide for the eye, in order to direct it in the axis of the lenses; it consists of two brass tubes, one sliding within the other, and a vertical flat piece, at the top of which is the hole for the eye. The outer tube is seen at MN, the vertical piece is represented at LM. The inner tube may be pulled out, or pushed in, to adjust it to the focus of the glasses. The vertical piece may be raised or depressed; that the hole, through which the object is to be viewed, may coincide with the centre in the field of view; it is fixed by a milled screw from M, which could not be shown in this figure.
At N is a dove-tailed piece of brass, made to receive the dove-tail at the end of the tubes MN, by which it is affixed to the wooden box ABCDE. The tubes MN may be removed from this box occasionally, for the convenience of packing it up in a less compass.
OP, a small tube which carries the magnifiers.
O, one of the magnifiers; it is screwed into the end of a tube, which slides within the tube P; the tube P may be unscrewed occasionally from the wooden body.
QRSTVX, a long square bar, which passes through the sockets YZ, and carries the stage or frame that holds the objects; this bar may be moved backward or forward, in order to adjust it to the focus by means of the pinion which is at a.
b, A handle furnished with an universal joint, for more conveniently turning the pinion. When the handle is removed, the nut (fig. 27) may be used in its stead.
d e, A brass bar, to support the curved piece KI, and keep the body AB firm and steady.
f g h i, The stage for opaque objects: it fits upon the bar QRST by means of the socket hi, and is brought nearer to or removed farther from the magnifying lens by turning the pinion a: the objects are placed in the front side of the stage (which cannot be seen in this figure) between four small brass plates; the edges of two of these are seen at k l. The two upper pieces of brass are moveable; they are fixed to a plate, which is acted on by a spiral spring, that presses them down, and confines the slider with the objects: this plate, and the two upper pieces of brass, are lifted up by the small nut m.
At the lower part of the stage, there is a semicircular lump of glass n, which is designed to receive the light from the lamp, fig. 29, and to collect and throw it on the concave mirror o, whence it is to be reflected on the object.
The upper part f g h s (fig. 26) of the opaque stage takes out, that the stage for transparent objects may be inserted in its place.
Fig. 28 represents the stage for transparent objects; the two legs 5 and 6 fit into the top of the under part r s h i of the stage for opaque objects; 7 is the part which confines or holds the sliders, and through which they are to be moved; 9 and 10, a brass tube, which contains the lenses for condensing the light, and throwing it upon the object; there is a second tube within that, marked 9 and 10, which may be placed at different distances from the object by the pin 11.
When this stage is used as a single microscope, without any reference to the lucernal, the magnifiers or object lenses, are to be screwed into the hole 12, and to be adjusted to a proper focus by the nut 13.
N.B. At the end AB (fig. 26.) of the wooden body there is a slider, which is represented as partly drawn out at A: when quite taken out, three grooves will be perceived; one of which contains a board that forms the end of the box; the next contains a frame with a grayed glass; and the third, or that farthest from the end AB, two large convex lenses.
Fig. 29 represents one of Argand's lamps, which Fig. 29. are most suitable for microscopic purposes, on account of the clearness, the intensity, and the steadiness of the light. The following account of the method of managing them, with other observations, is copied from an account given by Mr Parker with those he sells.
The principle on which the lamp acts, consists in disposing the wick in thin parts, so that the air may come in contact with all the burning fuel; by which means, together with an increase of the current of air occasioned by rarefaction in the glass tube, the whole of the fuel is converted into flame.
The wicks are circular; and, the more readily to regulate the quantity of light, are fixed on a brass collar, with a wire handle, by means of which they are raised or depressed at pleasure.
To fix the wick on, a wooden mandril is contrived, which is tapered at one end, and has a groove turned at the other.
The wick has a selvage at one end, which is to be put foremost on the mandril, and moved up to the groove; then putting the groove into the collar of the wick-holder, the wick is easily pushed forward upon it.
The wick-holder and wick being put quite down in their place, the spare part of the wick should, while dry, be set a-light, and suffered to burn to the edge of the tubes; this will leave it more even than by cutting, and, being black by burning, will be much easier lighted: for this reason, the black should never be quite cut off.
The lamp should be filled an hour or two before it is wanted, that the cotton may imbibe the oil and draw the better.
The lamps which have a reservoir and valve, need no other direction for filling than to do it with a proper trimming pot, carefully observing when they are full; then pulling up the valve by the point, the reservoir, being turned with the other hand, may be replaced without spilling a drop.
Those lamps which fill in the front like a bird-fountain, must be reclined on the back to fill; and this should Micro- should be done gently, that the oil in the burner may return into the body when so placed and filled; if, by being too full, and oil appears above the guard, only move the lamp a little, and the oil will disappear; the lamp may then be placed erect, and the oil will flow to its proper level.
The oil must be of the spermaceti kind, commonly called chamber oil, which may generally be distinguished by its paleness, transparency, and inoffensive scent: all those oils which are of a red and brown colour, and of an offensive scent, should be carefully avoided, as their glutinous parts clog the lamp, and the impurities in such oil, not being inflammable, will accumulate and remain in the form of a crust on the wick. Seal oil is nearly as pale and sweet as chamber oil; but being of a heavy sluggish quality, is not proper for lamps with fine wicks.
Whenever bad oil has been used, on changing it, the wick must also be changed; because, after having inhibited the coarse particles in its capillary tubes, it will not draw up the fine oil.
To obtain the greatest degree of light, the wick should be trimmed exactly even, the flame will then be completely equal.
There will be a great advantage in keeping the lamp clean, especially the burner and air tubes; the neglect of cleanliness in lamps is too common: a candlestick is generally cleaned every time it is used, so should a lamp; and if a candlestick is not to be objected to because it does not give light after the candle is exhausted, so a lamp should not be thought ill of, if it does not give light when it wants oil or cotton: but this last has often happened, because the deficiency is less visible.
The glass tubes are best cleaned with a piece of wash leather.
fountain lamp is left partly filled with oil, it may be liable to overflow: this happens by the contraction of the air when cold, and its expansion by the warmth of a room, the rays of the sun, or the heat of the lamp when re-lighted: this accident may be effectually prevented by keeping the reservoir filled, the oil not being subject to expansion like air. On this account, those with a common reservoir are best adapted for microscopic purposes.
To examine Opaque Objects, with the Lucernai Microscope. To render the use of this instrument easy, it is usually packed with as many of the parts together as possible: it occupies on this account rather more room, but is much less embarrassing to the observer, who has only three parts to put on after it is taken out of its box, namely, the guide for the eye, the stage, and the tube with its magnifier.
But to be more particular: Take out the wooden slider A (fig. 26.), then lift out the cover and the gray glass, from their respective grooves under the slider A.
Put the end N of the guide for the eye LMN into its place, so that it may stand in the position which is represented in this figure.
Place the socket which is at the bottom of the opaque stage, on the bar QXT, so that the concave mirror o may be next the end DE of the wooden body.
Screw the tubes PO into the end DE. The magnifier you intend to use is to be screwed on the end O of these tubes.
The handle G b, or the milled nut, fig. 27, must be placed on the square end of the pinion a.
Place the lamp lighted before the glass lamp n, and the object you intend to examine between the spring plates of the stage; and the instrument is ready for use.
In all microscopes there are two circumstances which must be particularly attended to: first, the modification of the light, or the proper quantity to illuminate the object; secondly, the adjustment of the instrument to the focus of the glass and eye of the observer. In the use of the lucernai microscope there is a third circumstance, which is, the regulation of the guide for the eye.
1. To throw the light upon the object. The flame of the lamp is to be placed rather below the centre of the glass lump n, and as near it as possible; the concave mirror c must be so inclined and turned as to receive the light from the glass lump, and reflect it thence upon the object; the best situation of the concave mirror and the flame of the lamp depends on a combination of circumstances, which a little practice will discover.
2. To regulate the guide for the eye, or to place the centre of the eye-piece L so that it may coincide with the focal point of the lenses and the axis of vision: Lengthen and shorten the tubes MN, by drawing out or pushing in the inner tube, and raising or depressing the eye-piece MI, till you find the large lens (which is placed at the end AB of the wooden body) filled by an uniform field of light, without any prismatic colours round the edge; for till this piece is properly fixed, the circle of light will be very small, and only occupy a part of the lens; the eye must be kept at the centre of the eye-piece L during the whole of the operation; which may be rendered somewhat easier to the observer, on the first use of the instrument, if he hold a piece of white paper parallel to the large lens, removing it from or bringing it nearer to them till he find the place where a lucid circle, which he will perceive on the paper, is brightest and most distinct; then he is to fix the centre of the eye-piece to coincide with that spot; after which a very small adjustment will set it perfectly right.
3. To adjust the lenses to their focal distance. This is effected by turning the pinion a, the eye being at the same time at the eye-piece L. The gray glass is often placed before the large lenses, while regulating the guide for the eye, and adjusting for the focal distance.
If the observer, in the process of his examination of an object, advance rapidly from a shallow to a deep magnifier, he will save himself some labour by pulling out the internal tube at O.
The upper part f g r s of the stage is to be raised or lowered occasionally, in order to make the centre of the object coincide with the centre of the lens at O.
To delineate objects, the gray glass must be placed before the large lenses; the picture of the object will be formed on this glass, and the outline may be accurately taken by going over the picture with a pencil. The opaque part may be used in the daytime without a lamp, provided the large lenses at A:B are screened from the light.
To use the Lucernal Microscope in the examination of Transparent Objects.—The instrument is to remain as before: the upper part figs of the opaque stage must be removed, and the stage for transparent objects, represented at fig. 28, put in its place; the end g 10 to be next the lamp.
Place the grayed glass in its groove at the end AB, and the objects in the slider-holder at the front of the stage; then transmit as strong a light as you are able on the object, which you will easily do by raising or lowering the lamp.
The object will be beautifully depicted on the gray glass; it must be regulated to the focus of the magnifier, by turning the pinion a.
The object may be viewed either with or without the guide for the eye. A single observer will see an object to the greatest advantage by using this guide, which is to be adjusted as we have described above. If two or three wish to examine the object at the same time, the guide for the eye must be laid aside.
Take the large lens out of the groove, and receive the image on the gray glass; in this case, the guide for the eye is of no use: if the gray glass be taken away, the image of the object may be received on a paper screen.
Take out the gray glass, replace the large lenses, and use the guide for the eye; attend to the foregoing directions, and adjust the object to its proper focus. You will then see the object in a blaze of light almost too great for the eye, a circumstance that will be found very useful in the examination of particular objects. The edges of the object in this mode will be somewhat coloured: but as it is only used in this full light for occasional purposes, it has been thought better to leave this small imperfection, than, by remedying it, to sacrifice greater advantages; the more so, as this fault is easily corrected, a new and interesting view of the object is obtained, by turning the instrument out of the direct rays of light, and permitting them to pass through only in an oblique direction, by which the upper surface is in some degree illuminated, and the object is seen partly as opaque, partly as transparent. It has been already observed, that the transparent objects might be placed between the slider-holders of the stage for opaque objects, and then be examined as if opaque.
Some transparent objects appear to the greatest advantage when the lens at g 10 is taken away; as, by giving too great a quantity of light, it renders the edges less sharp.
The variety of views which may be taken of every object by means of the improved Lucernal microscope, will be found to be of great use to an accurate observer: it will give him an opportunity of correcting or confirming his discoveries, and investigating those parts in one mode which are invisible in another.
To throw the image of transparent objects on a screen as in the solar microscope.—It has been long a microscopical desideratum, to have an instrument by which the image of transparent objects might be thrown on a screen, as in the common solar microscope: and this not only because the sun is so uncertain in this climate, and the use of the solar microscope requires confinement in the finest part of the day, when time seldom hangs heavy on the mind; but as it also affords an increase of pleasure, by displaying its wonders to several persons at the same instant, without the least fatigue to the eye.
This purpose is now effectually answered, by affixing the transparent stage of the lucernal to a lanthorn, with one of Argand's lamps.—The lamp is placed within the lanthorn, and the end g 10 of the transparent stage is screwed into a female screw, which is riveted in the sliding part of the front of the lanthorn; the magnifying lenses are to be screwed into the hole represented at 12, and they are adjusted by turning the milled nut. The quantity of light is to be regulated by raising and lowering the sliding-plate or the lamp.
Apparatus which usually accompanies the improved Lucernal Microscope.—The stage for opaque objects, with its semicircular lump of glass, and concave mirror. The stage for transparent objects, which fits on the upper part of the foregoing stage. The sliding tube, to which the magnifiers are to be affixed: one end of these is to be screwed on the end D of the wooden body; the magnifier in use is to be screwed to the other end of the inner tube. Eight magnifying lenses: these are so constructed that they may be combined together, and thus produce a very great variety of magnifying powers. A fish-pan, such as is represented at I. A steel wire L, with a pair of nippers at one end, and a small cylinder of ivory at the other. A slider of brass N, containing a flat glass slider, and a brass slider into which are fitted some small concave glasses. A pair of forceps. Six large and six small ivory sliders, with transparent objects. Fourteen wooden sliders, with four opaque objects in each slider; and two spare sliders. Some capillary tubes for viewing small animalcula.
en seldom content themselves with an instrument under one form; hence such a variety of microscopes, hence many alterations in the Lucernal Microscope. Mr Adams himself, we understand, has fitted up this last in a great many different ways; and it is reasonable to think that no person was more likely to give it every improvement of which it is susceptible. Of the alterations by other hands we shall only particularize one, made by Mr Jones of Holborn (B), whose description is as follows:
A (fig. 30.) represents a portion of the top of the mahogany Bogany box in which it packs, to preserve it steady; it slides in a dove-tail groove withinside, a similar groove to which is cut in the top of the box A; so that when the instrument is to be used, it is slid out of the box withinside, and then slid into the groove at top ready for use, almost instantly, as shown in the figure. The adjustment of the objects is at the stage E; for the right focal distance is readily and conveniently made by turning the long screw-rod BB, which goes through the two pillars supporting the box, and works in the base of the brass stage E; which base is also dovetailed, so as to have a regular and steady motion in another brass basis that supports it. In this instrument, therefore, the pyramidal box does not move; but the stage part only, which, from its small weight, moves in the most agreeable and steady manner. While observing the image of the object upon the glass through the sight-hole at G, the object may be moved or changed by only turning the rack-work and pinion applied to the stage by means of the handle D, for that purpose. By this contrivance you have no occasion to change your position during the view of the objects upon one of the sliders. This motion changes the objects horizontally only; and as they are generally placed exactly in one line, it answers all the purposes for which this motion is intended very well. But it may sometimes happen that the observer would wish to alter the vertical position of the object; to perform which there is another plain rod at F, that acts simply as a lever for this purpose, and moves the sliding part of the stage E vertically either upwards or downwards.
Thus, without altering his position, the observer may investigate all parts of the objects in the most satisfactory manner. Rack-work and pinion might be applied to the stage for the vertical motion also; but as it would materially enhance the expense, it is seldom applied. The brass work at the handle of D contains a Hooke's universal joint.
The brilliancy of the images of the objects shown upon the large lenses at the end of the box, being very frequently so great as to dazzle the eyes, Mr Jones applies a slight tinge of blue, green, and other coloured glass, to the sight-hole at G, which softens this glare, and casts an agreeable hue upon the objects.
f those Parts of a Microscopical Apparatus, common to most Instruments, which are delineated at fig. 31.
A and B represent the brass cells which contain the magnifiers belonging to the different kinds of compound microscopes. The magnifiers are sometimes contained in a slider like that which is delineated at S (fig. 24.). The lenses of A and B are confined by a small cap; on unscrewing this, the small lens may be taken out and cleaned. The magnifiers A of the lucernal microscope are so contrived, that any two of them may be screwed together, by which means a considerable variety of magnifying powers is obtained.
To get at the lenses in the slider S (fig. 24.) take out the two screws which hold on the cover.
C represents the general form of the slider-holder. It consists of a cylindrical tube, in which an inner tube is forced up by a spring. It is used to receive the ivory or any other slider, in which the transparent objects are placed; these are to be slid between the two upper plates: the hollow part in one of the plates is designed for the glass tubes.
D, the condensing lens and its tube, which fits into the slider-holder C, and may be moved up and down in it. When this piece is pushed up as far as it will go, it condenses the light of a candle, which is reflected on it by the plain mirror of the compound microscope, and spreads it uniformly over the object; in this case it is best adapted to the shallowest magnifiers. If the deeper lenses are used, it should be drawn down, or rather removed further from the object, that it may concentrate the light in a small compass, and thus render it more dense. The condensing lens is sometimes fitted up differently; but the principle being the same, it will be easy to apply it to use notwithstanding some variations in the mechanism.
E, a brass cone. It fixes under the slider-holder, and is used to lessen occasionally the quantity of light which comes from the mirror to any object.
F, a box with two flat glasses, which may be placed at different distances from each other in order to confine a small living insect.
G, a small brass box to hold the silver speculum H.
H, a small silver concave speculum, designed to reflect the light from the mirror on opaque objects; it should only be used with the shallow magnifiers. It is applied in different ways to the compound microscope; sometimes to a tube similar to that represented at X, which slides on the lower part of the body; sometimes it is screwed into the ring of the piece Q; the pin of this generally fits into one of the holes in the stage. When this speculum is used, the slider-holder should be removed.
I, a fish-pan, whereon a small fish may be fastened, in order to view the circulation of the blood: its tail is to be spread across the oblong hole at the smallest end, and tied fast by means of the ribbon fixed thereto, by shoving the knob which is on the back of it through the slit made in the stage; the tail of the fish may be brought under the lens which is in use.
K, a cylindrical piece, intended for the solar opaque microscope; by pulling back the spiral spring, smaller or larger objects may be confined in it.
L, a pair of triangular nippers for taking hold of and confining a large object.
M, a long steel wire, with a small pair of pliers at one end and a steel point at the other: the wire slips backwards or forwards in a spring tube, which is affixed to a joint, at the bottom of which is a pin to fit one one of the holes in the stage; this piece is used to confine small objects.
4. A small ivory cylinder that fits on the pointed end of the steel wire L; it is designed to receive opaque objects. Light-colored ones are to be stuck on the dark side, and vice versa.
M, a convex lens, which fits to the stage by means of the long pin adhering to it. This piece is designed to collect the light from the sun or a candle, and to throw them on any object placed on the stage; but it is very little used at present.
N, a brass slider, into which is fitted a flat piece of glass, and a brass slider containing four small glasses, one or two of them concave, the others flat; it is designed to confine small living objects, and when used is to be placed between the two upper plates of the slider-holder.
O, a glass tube to receive a small fish, &c.
P, represents one of the ivory sliders, wherein objects are placed between two pieces of tale, and confined by a brass ring.
Q, a piece to hold the speculum H: this piece is generally fitted to the microscope represented at fig. 12.
R, a pair of forceps, to take up any occasional object.
S, a camel's hair pencil to brush the dust off the glasses; the upper part of the quill is scooped out, to take up a drop of any fluid, and place it on either of the glasses for examination.
T, an instrument for cutting thin transverse sections of wood. It consists of a wooden base, which supports four brass pillars; on the top of the pillars is placed a flat piece of brass, near the middle of which there is a triangular hole.
A sharp knife, which moves in a diagonal direction, is fixed on the upper side of the afore-mentioned plate, and in such a manner that the edge always coincides with the surface thereof.
The knife is moved backwards and forwards by means of the handle a. The piece of wood is placed in the triangular trough which is under the brass plate, and is to be kept steady therein by a milled screw which is fitted to the trough; the wood is to be pressed forward for cutting by the micrometer screw b.
The pieces of wood should be applied to this instrument immediately on being taken out of the ground, or else they should be soaked for some time in water, to soften them so that they may not hurt the edge of the knife.
When the edge of the knife is brought in contact with the piece of wood, a small quantity of spirits of wine should be poured on the surface of the wood, to prevent its curling up; it will also make it adhere to the knife, from which it may be removed by pressing a piece of blotting paper on it.
9, An appendage to the cutting engine, which is to be used instead of the micrometer screw, being preferred to it by some. It is placed over the triangular hole, and kept flat down upon the surface of the brass plate, while the piece of wood is pressed against a circular piece of brass which is on the under side of it. This circular piece of brass is fixed to the screw, by which its distance from the flat plate on which the knife moves may be regulated.
An ivory box, containing at one end spare tale for the ivory slider, and at the other spare rings for pressing the tales together and confining them to the slider.
icroscopes have been also proposed; the first, it would appear, was suggested by Mr Grey. This was formed of water, and an account of it will be found in No. 221, 223, Phil. Trans. An improved microscope, on a similar principle, has been invented by Mr Brewster, of which the following is a description, taken from a note by the translator of Haüy's Natural Philosophy.
"A vertical bar (says Mr Gregory), is fixed upon a horizontal pedestal; and from the top of this bar proceeds a horizontal arm, which supports a circular case containing the lenses; below this another horizontal arm slides up and down, capable of adjustment by means of a screw, and carrying the usual sliders to hold the object which it is proposed to examine; and upon the pedestal is fixed the frame of a mirror, which has both an inclined and a horizontal motion, in order to illuminate any object upon the slider. The upper circular case is hollow, and contains four or more plano-convex lenses, which are constituted each of a drop of very pure and viscous turpentine varnish, taken up by the point of a piece of wood, and dropped upon a piece of very thin and well polished glass. The lenses thus formed may be made of any focal length by taking up a greater or a less quantity of fluid. The lower surface of the glass having been first smoked with a candle, the black pigment immediately below the lenses is then to be removed, so that no light may pass but through the lenses. The piece of glass is then perforated at its centre, and surrounded by a toothed wheel, which, when the wheel is put in the upper circular screw, may be turned by a common endless screw, so that the fluid lenses shall be brought severally under an eye-aperture properly disposed, and any object be successfully examined with a variety of magnifying powers." Note, p. 365. See also Ferguson's Lectures by Brewster, vol. ii.
After what has been related of microscopes, they cannot be said to be complete without the valuable addition of a micrometer; for the use and advantages of which, see the article MICROMETER.
Having presented our readers with descriptions of the various microscopes generally used, we think it our duty to point out to them those which we conceive to be best calculated to answer the purposes of science. The first which presents itself to our mind is that of Ellis: It is better adapted than any other portable microscope, to the purpose of general observation; simple in its construction, and general in its application. To those who prefer a double microscope, we should recommend that figured in Plate CCCXXXVIII. fig. 12. If opaque objects, as insects, &c., be subjects of investigation, the Lucernal Microscope claims the preference; but if amusement alone guides the choice, the Solar Microscope must be fixed upon.
We shall now proceed to explain some necessary particulars respecting the method of using microscopes; after which, we shall subjoin an enumeration of the prin- principal objects discovered or elucidated by their means.
On this subject Mr Adams, in his Essay on the Microscope, has been very copious; with a view, as he informs us, to remove the common complaint made by Mr Baker, "that many of those who purchase microscopes are so little acquainted with their general and extensive usefulness, and so much at a loss for objects to examine by them, that after diverting their friends some few times with what they find in the sliders which generally accompany the instrument, or perhaps with two or three common objects, the microscope is laid aside as of little further value; whereas no instrument has yet appeared in the world capable of affording so constant, various, and satisfactory an entertainment to the mind."
1. In using the microscope, there are three things necessary to be considered. (1.) The preparation and adjustment of the instrument itself. (2.) The proper quantity of light, and the best method of directing it to the object. (3.) The method of preparing the objects, so that their texture may be properly understood.
1. With regard to the microscope itself, the first thing necessary to be examined is, whether the glasses be clean or not: if they are not so, they must be wiped with a piece of soft leather, taking care not to soil them afterwards with the fingers; and, in replacing them, care must be taken not to place them in an oblique situation. We must likewise be careful not to let the breath fall upon the glasses, nor to hold that part of the body of the instrument where the glasses are placed with a warm hand; because thus the moisture expelled by the heat from the metal will condense upon the glass, and prevent the object from being distinctly seen. The object should be brought as near the centre of the field of view as possible; for there only it will be exhibited in the greatest perfection. The eye should be moved up and down from the eye-glass, of a compound microscope, till the situation is found where the largest field and most distinct view of the object are to be had; but every person ought to adjust the microscope to his own eye, and not to depend upon the situation it was placed in by another. A small magnifying power should always be begun with; by which means the observer will best obtain an exact idea of the situation and connexion of the whole; and will of consequence be less liable to form any erroneous opinion when the parts are viewed separately by a lens of greater power. Objects should also be examined first in their most natural position: for if this be not attended to, we shall be apt to form very inadequate ideas of the structure of the whole, as well as of the connexion and use of the parts. A living animal ought to be as little hurt or discomposed as possible.
From viewing an object properly, we may acquire a knowledge of its nature; but this cannot be done without an extensive knowledge of the subject, much patience, and many experiments; as in a great number of cases the images will resemble each other, though derived from very different substances. Mr Baker therefore advises us not to form an opinion too suddenly after viewing a microscopical object; nor to draw our inferences till after repeated experiments and examinations of the object in many different lights and positions; to pass no judgment upon things extended by force, or contracted by dryness, or in any manner out of a natural state, without making suitable allowances. The true colour of objects cannot be properly determined by very great magnifiers; for as the pores and interstices of an object are enlarged according to the magnifying power of the glasses made use of, the component particles of its substance will appear separated many thousand times farther asunder than they do to the naked eye: hence the reflection of the light from these particles will be very different, and exhibit different colours. It is likewise somewhat difficult to observe opaque objects; and as the apertures of the larger magnifiers are but small, they are not proper for the purpose. If an object be so very opaque, that no light will pass through it, as much as possible must be thrown upon the upper surface of it. Some consideration is likewise necessary in forming a judgment of the motion of living creatures, or even of fluids, when seen through the microscope; for as the moving body, and the space wherein it moves, are magnified, the motion will also be increased.
2. On the management of the light depends in a great measure the distinctness of the vision: and as, in order to have this in the greatest perfection, we must adapt the quantity of light to the nature of the object and the focus of the magnifier, it is therefore necessary to view it in various degrees of light. In some objects, it is difficult to distinguish between a prominence and a depression, a shadow or a black stain; or between a reflection of light and whiteness, which is particularly observable in the eye of the libellula and other flies: all of these appearing very different in one position from what they do in another. The brightness of an object likewise depends on the quantity of light, the distinctness of vision, and on regulating the quantity to the object; for some will be in a manner lost in a quantity of light scarcely sufficient to render another visible.
There are various ways in which a strong light may be thrown upon objects; as by means of the sun and a convex lens. For this purpose, the microscope is to be placed about three feet from a southern window; then take a deep convex lens, mounted on a semicircle and stand, so that its position may easily be varied: place this lens between the object and the window, so that it may collect a considerable number of solar rays, and refract them on the object or the mirror of the microscope. If the light thus collected from the sun be too powerful, it may be lessened by placing a piece of oiled paper, or a piece of glass lightly grayed, between the object and lens. Thus a proper degree of light may be obtained, and diffused equally all over the surface of an object: a circumstance which ought to be particularly attended to; for if the light be thrown irregularly upon it, no distinct view can be obtained. If we mean to make use of the solar light, it will be found convenient to darken the room, and to reflect the rays of the sun on the above-mentioned lens by means of the mirror of a solar microscope fixed to the window-shutter: for thus the observer will be enabled to preserve the light on his subject, notwithstanding the motion of the sun. But by reason of this motion, and the variable state of the atmosphere, solar observations are rendered both tedious and inconvenient: whence it will be proper for the observer to be furnished with a large tin lanthorn, formed something like the common magic lanthorn, capable of containing one of Argand's lamps. This, however, ought not to be of the fountain kind, lest the rarefaction of the air in the lanthorn should force the oil over. There ought to be an aperture in the front of the lanthorn, which may be moved up and down, and be capable of holding a lens; by which means a pleasant and uniform as well as strong light may easily be procured. The lamp should likewise move on a rod, so that it may be easily raised or depressed. This lanthorn may likewise be used for many other purposes; as viewing of pictures, exhibiting microscopic objects on a screen, &c. A weak light, however, is best for viewing many transparent objects; among which we may reckon the prepared eyes of flies, as well as the animalcules in fluids. The quantity of light from a lamp or candle may be lessened by removing the microscope to a greater distance from them, or by diminishing the strength of the light which falls upon the objects. This may very conveniently be done by pieces of black paper with circular apertures of different sizes, and placing a larger or smaller one upon the reflecting mirror, as occasion may require. There is an oblique situation of the mirrors, which makes likewise an oblique reflection of the light easily discovered by practice, (but for which no general rule can be given in theory); and which will exhibit an object more distinctly than any other position, showing the surface, as well as those parts through which the light is transmitted. The light of a lamp or candle is generally better for viewing microscopic objects than day light; it being more easy to modify the former than the latter, and to throw it upon the objects with different degrees of density.
3. Swammerdam has excelled in the preparation of objects almost all other investigators. Neither difficulty nor disappointment could make him abandon the pursuit of any object until he had obtained a satisfactory idea of it. But unhappily the methods he used in preparing his objects for the microscope are now entirely unknown. Boerhaave examined with the strictest attention all the letters and manuscripts of Swammerdam which he could find; but his researches were far from being successful. The following are all the particulars, which have thus come to the knowledge of the public.
For dissecting small insects, Swammerdam had a brass table made by S. Muschenbroeck, to which were affixed two brass arms moveable at pleasure to any part of it. The upper part of these vertical arms was constructed in such a manner as to have a slow vertical motion; by which means the operator could readily alter their height as he saw convenient. One of these arms was to hold the minute objects, and the other to apply the microscope.
The lenses of Swammerdam's microscopes were of various sizes as well as foci: but all of them the best that could be procured, both for the transparency of the glass and the fineness of the workmanship. His observations were always begun with the smallest magnifiers, from which he proceeded to the greatest; but in the use of them, he was so exceedingly dexterous, that he made every observation subservient to that which succeeded it, and all of them to the confirmation of each other, and to the completing of the description. His chief art seems to have been in constructing scissors of an exquisite fineness, and making them very sharp. Thus he was enabled to cut very minute objects to much more advantage than could be done by knives and lancets; for these, though ever so sharp and fine, are apt to disorder delicate substances by displacing some of the filaments, and drawing them after them as they pass through the bodies; but the scissors cut them all equally. The knives, lancets, and styles he made use of in his dissections, were so fine that he could not see to sharpen them without the assistance of a magnifying glass; but with these he could dissect the intestines of bees with the same accuracy that the best anatomists can do those of large animals. He made use also of very small glass tubes no thicker than a bristle, and drawn to a very fine point at one end, but thicker at the other. These were for the purpose of blowing up, and thus rendering visible, the smallest vessels which could be discovered by the microscope; to trace their courses and communications, or sometimes to inject them with coloured liquors.
Swammerdam sometimes made use of spirit of wine, water, or oil of turpentine, for suffocating the insects he wished to examine; and would preserve them for a time in these liquids. Thus he kept the parts from putrefying, and gave them besides such additional strength and firmness, as rendered the dissections much more easy than they would otherwise have been. Having then divided the body transversely with the scissors, and made what observations he could without farther dissection, he proceeded to extract the intestines carefully with very fine instruments, to wash away the fat in the like careful manner; and thus to put the parts into such a state as would best expose them to view; but these operations are best performed while the insects are in their nymphal state.
Sometimes the delicate viscera of the insects, after having been suffocated as above mentioned, were put into water; after which, having shaken them gently, he procured an opportunity of examining them, especially the air vessels, which last he could thus separate entire from all the other parts, to the admiration of all who beheld them: as these vessels cannot be distinctly seen in any other manner, or indeed in any way whatever, without injuring them. Frequently also he injected water with a syringe to cleanse the parts thoroughly, after which he blew them up with air and dried them; thus rendering them durable, and fit for examination at a proper opportunity. Sometimes he made very important discoveries, by examining insects which he had preserved for several years in balsam. Other insects he punctured with a very fine needle; and after squeezing out all their moisture through the holes made in this manner, he filled them with air, by means of very slender glass tubes; then dried them in the shade; and lastly, anointed them with oil of spike in which a little rosin had been dissolved; and by which means they for a long time retained their proper forms. He was likewise in possession of a singular secret, by which he could preserve the limbs of insects as limber and perspicuous as ever they had been. He used to make a small puncture or incision in the tails of worms; worms; and after having with great caution squeezed out all the humours, as well as great part of the viscera, he injected them with wax in such a manner as to give them the appearance of living creatures in perfect health. He found that the fat of all insects was entirely dissolvable in oil of turpentine; by which means he was enabled plainly to discern the viscera; though, after this dissolution, it was necessary to cleanse and wash them frequently in clean water. In this manner he would frequently have spent whole days in the preparation of a single caterpillar, and cleansing it from its fat, in order to discover the true situation of the insect's heart. He had a singular dexterity in stripping off the skins of caterpillars that were on the point of spinning their cones. This was done by letting them drop by their threads into scalding water, and then suddenly withdrawing them. Thus the epidermis peeled off very easily; and, when this was done, he put them into distilled vinegar and spirit of wine mixed together in equal proportions; which, by giving a due degree of firmness to the parts, gave him an opportunity of separating them with very little trouble from the exuviae, without any danger to the internal parts. Thus the nympha could be shown to be wrapped up in the caterpillar and the butterfly in the nympha; and there is little doubt that those who look into the works of Swammerdam, will be amply recompensed, whether they consider the unexampled labour or the piety of the author.
M. Lyonet, an eminent naturalist, usually drowned the insects he designed to examine; by which means he was enabled to preserve both the softness and transparency of the parts. According to him, the insect, if very small, viz. one-tenth of an inch, or little more, in length, should be dissected on a glass somewhat concave. If it should be suspected that the insect will putrefy by keeping for a few days, spirit of wine diluted with water must be substituted instead of pure water. The insect must be suffered to dry; after which it may be fastened by a piece of soft wax, and again covered with water.—Larger objects should be placed in a trough of thin wood; and for this purpose the bottom of a common chip box will answer very well; only surrounding the edge of it with soft wax, to keep in the water or other fluid employed in preserving the insect. The body is then to be opened; and if the parts are soft like those of a caterpillar, they should be turned back, and fixed to the trough by small pins, which ought to be set by a small pair of nippers. At the same time, the skin being stretched by another pair of finer forceps, the insect must be put into water, and dissected therein, occasionally covering it with spirit of wine. Thus the subject will be preserved in perfection, so that its parts may be gradually unfolded, no other change being perceived than that the soft elastic parts become stiff and opaque, while some others lose their colour.
The following instruments were made use of by M. Lyonet in his dissection of the Chenille de Saule. A pair of scissors as small as could be made, with long and fine arms: A pair of forceps, with their ends so nicely adjusted, that they could easily lay hold of a spider's thread, or a grain of sand: Two fine steel needles fixed in wooden handles, about two inches and three quarters in length; which were the most generally useful instruments he employed.
Dr Hooke, who likewise made many microscopic observations, takes notice, that the common ant or pismire is much more troublesome to draw than other insects, as it is extremely difficult to get the body in a quiet natural posture. If its feet be fettered with wax or glue, while the animal remains alive, it so twists its body, that there is no possibility of gaining a proper view of it; and if it be killed before any observation is made, the shape is often spoiled before it can be examined. The bodies of many minute insects, when their life is destroyed, instantly shrivel up; and this is observable even in plants as well as insects, the surface of these small bodies being affected by the least change of air; which is particularly the case with the ant. If this creature, however, be dropped into rectified spirit of wine, it will instantly be killed; and when it is taken out, the spirit of wine evaporates, leaving the animal dry, and in its natural posture, or at least in such a state, that it may easily be placed in whatever posture we please.
Parts of Insects. The wings, in many insects, are so transparent, that they require no previous preparation; but some of those that are folded up under elytra or cases, require a considerable share of dexterity to unfold them; for these wings are naturally endowed with such a spring, that they immediately fold themselves again, unless care be taken to prevent them. The wing of the earwig, when expanded, is of a tolerable size, yet is folded up under a case not one-eighth part of its bulk; and the texture of this wing renders it difficult to be unfolded. This is done with the least trouble immediately after the insect is killed. Holding then the creature by the thorax, between the finger and the thumb, with a blunt-pointed pin endeavour gently to open it, by spreading it over the forefinger, and at the same time gradually sliding the thumb over it. When the wing is sufficiently expanded, separate it from the insect by a sharp knife or a pair of scissors. The wing should be pressed for some time between the thumb and finger before it be removed; it should then be placed between two pieces of paper, and again pressed for at least an hour, after which time, as there will be no danger of its folding up any more, it may be put between the tacks, and applied to the microscope. Similar care is requisite in displaying the wings of the notonecta and other water insects, as well as most kinds of grilli.
The minute scales or feathers, which cover the wings of moths or butterflies, afford very beautiful objects for the microscope. Those from one part of the wing frequently differ in shape from such as are taken from other parts; and near the thorax, shoulder, and on the fringes of the wings, we generally meet with hair instead of scales. The whole may be brushed off the wing, upon a piece of paper, by means of a camel's hair pencil; after which the hairs can be separated with the assistance of a common magnifying glass.
It is likewise a matter of considerable difficulty to dissect properly the proboscis of insects, such as the gnat, tabanus, &c. and the experiment must be repeated a great number of times before the structure and situation tuation of the parts can be thoroughly investigated, as the observer will frequently discover in one what he could not in another. The collector of the bee, which forms a very curious object, ought to be first carefully washed in spirit of turpentine; by which means it will be freed from the unctuous matter adhering to it: when dry, it is again to be washed with a camel's hair pencil to disengage and bring forward the small hairs which form part of this microscopic beauty. The best